Chicory abrogates oxidative stress, inflammation and caspase-dependent apoptosis in acute hepatic injury model induced by acetaminophen in rats

Authors

  • Mohamed Elsayed Nady Pharmacology and Toxicology Department, Faculty of Pharmacy, Al-Azhar University.
  • Ahmed M. Mansour Pharmacology and Toxicology Department, Faculty of Pharmacy, Al-Azhar University, Nasr-City, Cairo, Egypt.
  • Elsayed E. Hafez Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, New Borg El-Arab City 21934, Alexandria, Egypt.
  • Gamal Omran Biochemistry Department, Faculty of Pharmacy, Damanhour University, Egypt
  • Gamal M. Hamad Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, New Borg El-Arab City 21934, Alexandria, Egypt.
  • Sahar E. Harraz National Research Centre (NRC), Giza, Egypt
  • Shady N. Allam Pharmacology and Toxicology Department, Faculty of Pharmacy, Al-Azhar University, Nasr-City, Cairo, Egypt.
  • Ali A. Ahamad Pharmacology Department, Faculty of Medicine, Al-Azhar University, Asut branch, Egypt.

Keywords:

Acetaminophen, Antioxidant, Apoptosis, Cichorium intybus, Hepatotoxicity, Silymarin

Abstract

In this study the protective effect of chicory leaves hydroalcoholic extract (CIE) against acute liver injury induced by a single dose of acetaminophen (700 mg/kg, i.p.) was investigated in rats. The CIE and silymarin treatment (standard reference) were given in a dose of (100 mg/kg, p.o.) for 3 days before and at 1 and 12 h following acetaminophen administration. Treatment with CIE significantly reduced the levels of serum ALT, AST, alkaline phosphatase, bilirubin, total cholesterol, triglycerides, urea, creatinine, TNF-α and hepatic contents of malondialdehyde (MDA), nitric oxide, caspase-3 and hydroxyproline, with significant increases in serum total protein, albumin, HDL- cholesterol and hepatic activities of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) as compared with the acetaminophen group. The histopathological alterations mediated by acetaminophen were ameliorated by CIE. It was concluded that CIE protects rat liver against acetaminophen hepatotoxicity, most probably through abrogation of oxidative stress, inflammation and caspase-3 dependent apoptosis.

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Published

31-03-2016

How to Cite

1.
Mohamed Elsayed Nady, Ahmed M. Mansour, Elsayed E. Hafez, Gamal Omran, Gamal M. Hamad, Sahar E. Harraz, Shady N. Allam, Ali A. Ahamad. Chicory abrogates oxidative stress, inflammation and caspase-dependent apoptosis in acute hepatic injury model induced by acetaminophen in rats. ijp [Internet]. 2016 Mar. 31 [cited 2024 Nov. 2];8(1):13-21. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/441

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