Identification of an antibacterial Withanolide (Dinoxin B) from leaf of Datura inoxia Mill.


  • Chandni Tandon Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow- 226028
  • Priti Mathur Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow- 226028.
  • Manodeep Sen Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow- 226010 India.
  • Sanjeev Kanojiya Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow-226031


Datura inoxia, antibacterial compound, column chromatography, LC-ESI-MS, Withanolide


Natural medicines are gaining popularity due to the alarming incidence of bacterial resistance even in regions with improved healthcare systems. Hence, there is a distinct and constant need for isolating medicinally active compounds from different natural sources. Taking this into consideration, experiment was done in order to isolate and identify an antibacterial compound from Datura inoxia through bioassay and Mass spectroscopy technique. The ethanol, hexane and aqueous leaf extracts of Datura inoxia were screened for


. Akinyemi KO, Oladapo O, Okwara CE,

Ibe C, Fasure KA. Screening of crude

extracts of six medicinal plants used in

South-West Nigeria unorthodox

medicine for antimethicilin resistantStaphylococcus aureus activity. BMC

Compl Alt Med. 2005; 5: 5-8.

. Mahady GB. Global harmonization of

herbal health claims. J Nutr. 2001; 131:


. Austan DJ, Kristinsson KG, Anderson

RM. The relationship between the

volume of antimicrobial consumption in

human communities and the frequency

of resistance. Tro J Phar R. 1999; 3(2):


. Bachi BB. Resistance mechanisms of

Gram positive bacteria. J Med Microb.

; 292: 27-35.

. Nagi AAH, Mashan NI, Shamsudin MN,

Mohamad H, Vairappan CS.

Antibacterial activity of marine source

extracts against multidrug resistance

organisms. Am J Pharm Toxicol. 2010;

: 95-102.

. Paul C, Arnold JV, Dirk VB, Louis M.

Anti-infective potential of natural

products: How to develop a stronger in

vitro ‘proof-of-concept’. J

Ethnopharmacol. 2006; 106: 290-302.

. Abutbul S, Golan-Goldhirsh A, Barazani

O, Ofir R, Zilberg D. Screening of desert

plants for use against bacterial

pathogens in fish. Isr J Aquacult

Bamidgeh. 2005; 57: 71-80.

. Finch R, Hunter PA. Antibiotic

resistance‐action to promote new

technologies. J Antimicrob Chemother.

, 58(suppl 1): 3‐22.

. Ariyo AO. Antimicrobial activity of the

indigenous liverwort, Riccia nigerica

Jones, from Southwestern Nigeria.

Evansia. 2011; 28(2): 43‐48.

. Gerald B. Biologically active

compounds from marine organisms.

Phytother Res. 2001; 15: 89-94.

. Houghton PJ. Old yet newpharmaceuticals from plants. J Chem

Educ. 2001; 78: 175.

. Duke JA, Ayensu ES. Medicinal plants

of China. Houghton Mifflin China. 1985:

– 91.

. Ali M, Shuab M. Characterization of the

chemical constituents of Datura metel

Linn. Ind J Pharm Sci. 1996; 5(6): 243 -

. Dabur R, Ali M, Sigh H, Gupta J,

Sharma G. A novel antifungal pyrole

derivative from Datura metel.

Pharmazie CODEN Pharlet. 2004; 59:

- 570.

. Chopra RN, Nayar SL, Chopra LC.

Glossary in Indian medicinal plants.

Council of Scientific and Industrial

Research: New Delhi; 1968. p. 121-

. Chopra RN, Nayar SL, Chopra LC.

Glossary of Indian medicinal plants.

Council of Scientific and Industrial

Research: New Dehli; 1986. p. 238 -

. Preissel U, Preissel HG. Brugmansia

and Datura: Angel's Trumpets and

Thorn Apples. Buffalo, NY: Firefly

Books; 2002. p. 106-129.

. Mathur P, Singh A, Srivastava V, Singh

D, Mishra Y. Antimicrobial activity of

indigenous wildly growing plants:

Potential source of green antibiotics. Afr

J Microbiol Res. 2013; 7(29): 3807-

. Jamdhade M, Survase S, Kare M,

Bhuktar A. Antibacterial activity of genus

Datura L. in Marathwada. Maharashtra

J phytol. 2010; 2(12): 42-45.

. Bharathi B, Sharmila devi R, Daniel G.

Studies on antibacterial activity and

phytochemical analysis of Datura metel

L against bacterial pathogens

associated with HIV. J Advanced

Biotech. 2010; 10(3): 21-25.

. Sakthi S, Saranraj P, Geetha M.

Antibacterial evaluation and

phytochemical screening of Datura

metel leaf extracts against bacterial

pathogens. Int J Pharm Biol. 2011; 2(4):


. Tandon C, Mathur P, Sen M.

Antibacterial compounds in different

species of Datura: A review. 2014;

(21): 2090-2098.

. Okwu D, Igara E. Isolation,

characterization and antibacterial

activity of alkaloid from Datura metel

Linn leaves. Afr J Pharm Pharmacol.

; 3(5): 277-281.

. Okwu D, Igara E. Isolation,

characterization and antibacterial

activity screening of a new ß-carboline

alkaloid from Datura metel Linn. Der

Chemica Sinica. 2011; 2(2): 261-267.

. Sule A, Ahmed QU, Samah OA, Omar

MN, Hassan N, Kamal L, Yarmo M.

Bioassay guided isolation of

antibacterial compounds from

Andrographis paniculata. Am J Applied

Sci. 2011; 8(6): 525-534.

. Sen M. Authored chapter in the Manual

entitled “Vancomycin Resistant

Enterococci” of CME/Workshop on

update in Clinical Microbiology theme-“

Emerging antimicrobial resistance: Are

we doing enough?” conducted by

Department of Microbiology, Sanjay

Gandhi Post Graduate Institute of

Medical Sciences, Lucknow in

November 2006.

. Perez C, Paul M, Bazerque P. Antibiotic

assay by well diffusion method. Acta

Biol Med Expt. 1990; 15: 113-115.

. Carson CF, Hammer KA, Riley TV.

Broth micro-dilution method for

determination of susceptibility of

Escherichia coli and Staphylococcus

aureus to the essential oil of Malaleuca

alterifolia (Tea tree oil). Microbios. 1995;

: 181–185.

. Kataky A, Handique PJ. Antimicrobial

activity and phytochemical estimation of

micropropagated Andrographis

paniculata (Burm.f) NEES. Asian

Journal of Science and Technology.

; 5: 91-94.

. Mims and Playfair. Medical

microbiology. Mosby Europe. 1993; 35:

. Irkin R, Korukluoglu M. Control of

Aspergillus niger with garlic, onion and

leek extracts. Afr J Biotechnol. 2007; 6:


. Gatsing D, Nkeugoauapi CFN, Nkah

BFN, Kuiate JR, Tchouanguep FM.

Antibacterial activity, bioavailability and

acute toxicity evaluation of the leaf

extract of Alchornea cordifolia

(Euphorbiaceae). Int. J. Pharmacol.

; 6: 173–182.

. Hosamani PA, Lakshman HC, Sandeep

KK, Hosamani RC. Antimicrobial activity

of leaf extract of Andrographis

paniculata wall. Science Research

Reporter. 2011; 1(2): 92-95

. Eloff JN. Which extractant should be

used for the screening and isolation of

antimicrobial components from plants?

J. Ethnopharmacol. 1998; 60: 1–8.

. Paz EA, Cerdeiras MP, Fernandez J,

Ferreira F, Moyna P. Screening of

Uruguayan medicinal plants for

antimicrobial activity. J

Ethnopharmacol. 1995; 45(1): 67-70.

. Kudi AC, Umoh JU, Eduvie LO, Gefu J.

Screening of some Nigerian medicinal

plants for antibacterial activity. J

Ethnopharmacol. 1999; 67(2): 225-228.

. Palombo EA, Semple SJ. Antibacterial

activity of traditional Australian

medicinal plants. J. Ethnopharmacol.

; 77(2-3): 151-157.

. Karaman I, Sahin F, Gulluce M, Ogutcu

H, Sngul M, Adiguzel A. Antimicrobial

activity of aqueous and methanol

extracts of Juniperus oxycedrus L. J

Ethnopharmacol. 2003; 85(2-3): 231–

. Nikaido H. Molecular basis of bacterial

outer membrane permeability revisited.

Microb Mol Bio Rev. 2003; 67: 593-656.

. Tortora GJ, Funke BR, Case CL.

Microbiology: An Introduction. 7th ed.

Benjamin Cummings Publishing: San

Francisco, USA; 2001. p. 88.

. Gurinder JK, Daljit SA. Antibacterial and

phytochemical screening of Anethum

graveolens, Foeniculum vulgare and

Trachyspermum ammi. BMC

Complement Altern Med. 2009; 9: 30.

. Stavri M, Piddock LVJ, Gibbons S.

Bacterial efflux pump inhibitors from

natural sources. J Antimicrobial Chemo.

; 59(6): 1247-1260.

. Doughari JH, Manzara S. In vitro

antibacterial activity of crude leaf

extracts of Mangifera indica Linn. Afr J

Microbiol Res. 2008; 2: 67-72.

. Vermillion K, Holguin OF, Berhow MA,

Richins RD, Redhouse T, O’Connell

MA, Posakony J, Mahajan SS, Kelly

SM, Simon JA. Dinoxin B, a Withanolide

from Datura inoxia leaves with specific

cytotoxic activities. J Nat Prod. 2011;

(2): 267–271.

. Abraham A, Kirson I, Lavie D, Glotter E.

The Withanolides of Withania somnifera

chemotypes I and II.

Phytochemistry.1975; 14: 189–194.

. Manickam M, Srivastava A, Ray A.

Withanolides from the flowers of Datura

fastuosa. Phytochemistry .1998; 47:


. Evans WC. In Solanaceae Biology and

Systematics: D’Arcy WG, editor.

Columbia University Press: New York;

p. 179-186.

. Veleiro AS, Cirigliano AM, Oberti JC,

Burton G. 7-Hydroxywithanolides from

Datura ferox. J Nat Prod. 1999;


. Siddiqui BS, Afreen S, Begum S. Two

New Withanolides from the aerial parts

of Datura inoxia. Aust J Chem.1999; 52:


. Siddiqui BS, Afreen S, Begum S, Sattar

F. Daturacin, a new withanolide from

Datura inoxia. Nat Prod Res.2005; 19:


. Gupta M, Bagchi A, Ray AB. Additional

Withanolides of Datura metel. J Nat

Prod. 1991; 54: 599–602.

. Pan Y, Wang X, Hu X. Cytotoxic

Withanolides from the flowers of Datura

metel. J Nat Prod. 2007; 70: 1127–

. Kagale S, Marimuthu T, Thayumanavan

B, Nandakumar R, Samiyappan R.

Induction of systemic resistance in rice

by leaf extracts of Zizyphus jujuba and

Ipomoea carnea against Rhizoctonia

solani. Plant Signal Behav. 2011; 6(7):





How to Cite

Chandni Tandon, Priti Mathur, Manodeep Sen, Sanjeev Kanojiya. Identification of an antibacterial Withanolide (Dinoxin B) from leaf of Datura inoxia Mill. ijp [Internet]. 2016 Mar. 31 [cited 2023 Nov. 29];8(1):1-12. Available from:



Original Research Articles