Phytochemical, antibacterial and antifungal activity of rhizome from Anaphyllum wightii.schott against clinical isolates and plant pathogens

Authors

  • Manesh Kunjumon DEPT. OF BIOTECHNOLOGY UNIVERSITY OF KERALA KARIAVATTOM CAMPUS TRIVANDRUM India.
  • ShirlyK Thomas Dept. of Biotechnology, University of Kerala,Kariavattom, Thiruvananthapuram 695581, Kerala, India.
  • Roshin Elizabeth George Dept. of Biotechnology, University of Kerala,Kariavattom, Thiruvananthapuram 695581, Kerala, India.
  • Vaidyanatha Iyer Thankamani Dept. of Biotechnology, University of Kerala,Kariavattom, Thiruvananthapuram 695581, Kerala, India

Keywords:

Anaphyllumwightii, antibacterial, antifungal, Plant pathogen, Rhizome, Plant pathogenMIC, MBC

Abstract

Anaphyllumwightii. Schott is a very rare and endemic threatened plant belonging to the family Areacea. It has been used in the tribal medicine to treat eczema, scabies and as antidote against snakebite.Different solvent extracts of Anaphyllumwightii.Schott were prepared and analysed for phytochemical screening by standard protocols. Antimicrobialactivity was measured using well diffusion method and micro dilution broth method against clinical isolates. The n-butanol and methanol extracts of A. wightii showedsignificant activity against all of the selected microorganisms. Water extract did not show any activity against any of the organisms except Klebsiella sp.The MIC value for leaf n- butanol and methanol were 1.5 mg/ml to 2.5mg/ml and the MBC values were 1.25 to 5 5mg/ml respectively.The antimicrobial activity of rhizome observed in the present study may provide the suggestion that the plant could be a potential source of new and effective antibacterial & antifungal agents. Hence further studies areproposed for the isolation of the antibacterial & antifungal agents from the rhizome extracts of A. wightii.

References

. Ahmad I, Mehmood Z, Mohammad F.

Screening of some Indian medicinal

plants for their antimicrobial properties.

J Ethnopharmacol.1998; 62,183-193.

. Murray M. The Healing power of herbs.

Prima Publishing, CA, 1995; 162-171

. Nayar, MP. Key works to the taxonomy

of flowering plants of India. Botanical

Survey of India (1984)

. Dominic VJ. Biosystematic studies in

the genus Anaphyllum Schott

(Araceae) of Western ghats. Int. J.

LifeSc. Bt& Pharm. Res. 2012; 3, 161-

. ArunVijayan, Liju VB, Reena Johan JV,

Parthipan B and Reenuka C.

Traditional remedies of Kani Tribes of

Kottoor reserve forest,

Agasthyavanam, Kerala. Indian Journal

of Traditional Knowledge. 2007; 6

(4),589-594.

. Udayan PS, Satheesh George KV

Tushar and Indira

Balachandran.Ethanomedicine of

Malapandaram tribes of Achankovil

forest of Kollam district, Kerala. Journal

of Traditional Knowledge. 2007; 6(4),

-573.

. Ramachandran VS. Wild edible plants

of the Anamalais, Coimbatore district,

Western Ghats, Tamil Nadu. In Indian

J. Traditional Knowledge. 2007; 6(1),

-176.

. Jose Mathew and George KV.

Medicinal plants used by

malampadaramtribals in achankovil

forests of kerala to cure snake bite,

wound and bone fracture. Life

Sciences Leaflets. 2013; 8, 50-54.

. Rajagopala PL, RobinJoseaK,

Premalethab KD, Kironc SS, DR.K.R,

Sreejithd , Aneeshiae S.Anthelmintic

studies on the rhizomes of

Anaphyllumwightiischott. Journal of

international academic research for

multidisciplinary. 2013; 1(11), 645-649.

. RajagopalPL, SreejithKR, KironSSand

NarayanaSwami VB.Anti-inflammatory

activity of the rhizomes of

Anaphyllumwightii Schott. American

Journal of Pharmacy & Health

Research. 2013; 1(6),76-80.

. Dharsana JN. Molly Mathew.

Premkumar N. Diljit S Kuttoor.

Preliminary screening of

anaphyllumwightiischott tubers for antiinflammatory and antioxidant

activity.Am. J. PharmTech Res. 2014;

(4), 88-97.

. Rajagopal PL, Sreejith KR, Kiron SS

and Narayana Swami VB PL.

Antidiabetic activity (invitro) of the

rhizomes of anaphyllumwightii.schott.

International journal of research in

pharmacy and chemistry. 2013, 3(4).

-792.

. Molly Mathew, dharsana JN, Soumya K

Vijayan, Premkumar N. Anti-diabetic

activity of anaphyllumwightiischott in

alloxan induced diabetic rats. Asian

Journal of Pharmaceutical and Clinical

Research. 2013; 6(1), 68-69.

. HarborneJB. Phytochemical methods.

nd ed. LondonăNew York: Chapman

and Hall, 1984.p.(5th ed. Bangalore,

India: Prism Books (Pvt.) Ltd.; 1994; p.

ă6.

. John V. Bennett, Jean L Brodie,

Ernest J Benner, and William MM

Kirby. Simplified, accurate method for

antibiotic assay of clinical specimens.

Applied Microbiology, 1966; 14 (2).171

-17

. National Committee for Clinical

Laboratory Standards, Methods for

Dilution Antimicrobial Susceptibility

Test for Bacteria that Grow Aerobically.

Document M07-A8, 8th ed. Wayne,

Philadelphia: National Committee for

Clinical LaboratoryStandards; 2012.

. National Committee for Clinical

Laboratory Standards. Methods for

dilution antimicrobial susceptibility tests

for bacteria that grow aerobically.

Approved standard M7-A5, 5th ed.

Wayne, Philadelphia: National

Committee for Clinical

LaboratoryStandards; 2000.

. Pandey BP. Fungi,

S.Chand&Company, New Delhi,1994;

p.416

. Dominic VJ and ManjuMadhavan.

Pharmacognostic and preliminary

phytochemical Screening of the

rhizome of anaphyllumwightiischott, an

endemic and threatened genus of the

Western Ghats. Journal of Herbal

Medicine and Toxicology. 2012; 6 (2),

ă 60.

. ThankamaniV,JamesJ,Dev L.

Phytochemical screening and

antibacterial activity of

Alstoniascholaries flower. In.J.Pharm

development. 2011; 3(4), 172-178.

. Shirly K Thomas, Roshin Elizabeth

George, ManeshKunjumon,

ThankamaniV.In vitro antibacterial

profile of Alstoniavenenata r. Br- a

comparative study. Int J Curr Pharm

Res. 2014; 6(4), 58-60

. Cowan. MM, „Plant products as

antimicrobial agents,‰ Clinical

microbiology reviews. 1999; 12, p.

‐82.

. Bonjar GHS, Nik AK and Aghighi S:

Antibacterial and Antifungal survey in

plants used in indigenous herbalmedicine of south east regions of Iran.

J Biol Sci. 2004; 4: 405-412.

. Nain P, SainiV,Sharma S In-vitro

antibacterial and antioxidant activity of

Emblicaofficinalis leaves extract. Int J

Pharm PharmSci, 2012; 4(1), 385-389.

. Marjorie C: Plant products as

Antimicrobial agents. Clinical

Microbiology Reviews 1999; 12: 564-

. Hoult J.R.S and Paya M:

Pharmacological and biochemical

actions of simple coumarins: 1native

products with therapeutic potential.

Gen Pharmacol 1996; 27: 713-722.

. Doughari JH, Elmahmood AM,

Manzara S. Studies on the antibacterial

activity of root extracts of Carica

papaya L. African Journal of

Microbiology Research 2007; 1(3), 37-

. Roy Saswati, DuttaChoudhuryM,and

Paul SB. Antimicrobial activity of

Araceae: an overview. IJRAP.

;4(1), 15-17.

. Rasooli.SA, Mirmostafa.

Antimicrobialactivityof thymus

pubescens and T. Seyphyllum

essential oils. Fitoterapia, 2002; 73(3),

ă250.

. Saleem M, Nazir M, Ali MS, Hussain H,

Lee Y S, Riaz N. Antimicrobial natural

products: An update on future antibiotic

drug candidates. Natural Product

Reports, et al. (2010) 238, 254

. Tsuchiya H, Sato M, Miyazaki T,

Fujiwara S, Tanigaki S, Ohyama M,

Tanaka T.andLinuma M. Comparative

study on the antibacterial activity of

phytochemical flavanones against

methicillin-resistant Staphylococcus

aureus. J. Ethnopharmacol. 1996.50,

-34.

. Kim SJ, Cho A, Han J. Antioxidant and

antimicrobial activity of leafygreen

vegetable extracts and their

applications to meat product

preservation.Food Control. 2013; 29,

ă120.

. Purkayastha S, Dahiya P.

Phytochemical analysis and

antibacterial efficacy of babchi oil

(Psoraleacorylifolia) against multi-drug

resistant clinical isolates. International

Conference on Bioscience,

Biochemistry and

Bioinformatics.IPCBEE 2012; 3(1): 64-

. Chanda S, Kaneria M. Indian

nutraceutical plant leaves as a potential

source of natural antimicrobial agents,

in: A. Mendez Vilas (Ed.), Science

against Microbial Pathogens:

Communicating Current Research and

Technological Advances, Fomatex

Research CenterPublication,

Spain,2011;1251ă1259.

. Chanda S, Dudhatra S, Kaneria M.

Antioxidative and antibacterial effects

of seeds and fruit rind of nutraceutical

plants belonging to the Fabaceae

family, Food. Funct. (2010);1 (3), 308ă

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Published

31-12-2015

How to Cite

1.
Manesh Kunjumon, ShirlyK Thomas, Roshin Elizabeth George, Vaidyanatha Iyer Thankamani. Phytochemical, antibacterial and antifungal activity of rhizome from Anaphyllum wightii.schott against clinical isolates and plant pathogens. ijp [Internet]. 2015 Dec. 31 [cited 2024 May 21];7(4):459-67. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/438

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