Plasmatic antioxidant capacity as a possible marker of phytodrugs efficacy


  • Andrea Muller PhD candidate in Pharmacology, University of Chile.
  • I. Saavedra Saavedra Center of Pharmacological and Toxicological Research, Program of Molecular and Clinical Pharmacology, Faculty of Medicine, University of Chile, Carlos Schatebeck 299, ParqueQuinta Normal , Santiago, Chile.
  • M. E. Letelier Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Sergio Livingstone Pohlhammer (ex–Olivos) 1007, Independencia, Santiago, Chile.


Buddleja globosa Hope, Plantago major L., Lipid oxidation, Thiol oxidation, Ferric reducing ability of plasma


A correlation between drug plasma concentration and its therapeutic effect exist. Then we postulate that determination of plasmatic antioxidant capacity could prove phytodrugs efficacy. Therefore, we determined the plasmatic ability to reduce Fe3+ and malondialdehyde concentration after orally administration of herbal extracts to rats. For this, we use hydro-alcoholic extracts of Buddleja globosa Hope and Plantago major L. previously characterized according to their polyphenol and thiol compounds contents, and their capacities to inhibit the oxidation of microsomal lipid and thiol compounds. The amounts of polyphenol and thiol compounds were three times higher in B. globosa than in P. major extracts. Moreover, EC50 of B. globosa extract in preventing oxidation of microsomal lipid and thiol compounds induced by Cu2+/ascorbate were 3 times lower. These extracts were also able to inhibit microsomal GSH-transferase activity and chelate Cu2+. The oral administration of these extracts to rats provoked an increase in the ability to reduce Fe3+ and a decrease in malondialdehyde concentration. Since the antioxidant activity of these extracts was reproduced in vivo, we believe that the efficacy of phytodrugs used in complementary therapies may be evaluated by measuring the plasma antioxidant capacity.


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How to Cite

Andrea Muller, I. Saavedra Saavedra, M. E. Letelier. Plasmatic antioxidant capacity as a possible marker of phytodrugs efficacy. ijp [Internet]. 2015 Dec. 31 [cited 2024 May 19];7(4):379-87. Available from:



Original Research Articles