Hepatoprotective and antioxidant effects of “Couroupita guianensis” on carbon tetra chloride - induced liver damage


  • Raja Sundararajan GITAM Institute of Pharmacy, GITAM University, Visakhapatnam- Andhra Pradesh, India-Pincode-530 045 India
  • Ravindranadh Koduru GITAM Institute of Pharmacy, GITAM University, Visakhapatnam- Andhra Pradesh, India-Pincode-530 045 India.


Couroupita guianensis, serum transaminases, carbon tetrachloride, hepatoprotective, histopathology


To investigate the hepatoprotective and antioxidant activities of methanol leaves extract of Couroupita guianensis against carbon tetrachloride+ Olive oil -induced hepatotoxicity in rats. The hepatoprotective activity of methanol extract of leaves of Couroupita guianensis was evaluated against carbon tetrachloride + Olive oil -induced hepatic damage in rats. In-vivo antioxidant activity of methanol extract of leaves of Couroupita guianensis was evaluated by various assays including lipid peroxidation, super oxide dismutase, reduced glutathione, catalase, glutathione peroxidase, and glutathione reductase. The methanol extract of leaves of Couroupita guianensis at dose of 250 and 500 mg/kg were administered orally once daily for seven days. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphatase (SALP), bilirubin and Lactate dehyrogenase were estimated along with estimation of superoxide dismutase (SOD), reduced glutathione, catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GRD) and malondialdehyde (MDA) levels in liver tissues. Further histopathological examination of the liver sections was carried out to support the induction of hepatotoxicity and hepatoprotective efficacy. The substantially elevated serum enzymatic levels of SGOT, SGPT, ALP total bilirubin and total protein were found to be restored towards normalization significantly by the methanol extract of leaves of Couroupita guianensis in a dose dependent manner with maximum hepatoprotection at 500mg/kg dose level. The extract showed potent activities on reduced glutathione, SOD, CAT, GRD, GPx and MDA levels. The histopathological observations supported the biochemical evidences of hepatoprotection. The results of the present study strongly reveal that methanol extract of leaves of Couroupita guianensis has hepatoprotective and antioxidant activities against carbon tetrachloride -induced hepatic damage in experimental animals.


. Aebi H. Catalase. In Methods in

enzymatic analysis. Vol. 2, ed. H.V.

Bergmeyer. New York,

Cheime,Weinheim, FRG: Academic

press. 1974. 674ă684.

. Ashok Shenoy K, Somayaji SN, Bairy

KL. Hepatoprotective effects of Ginkgo

biloba against carbon tetrachloride

induced hepatic injury in rats. Indian J

Pharmacol 2001; 33: 260-266.

. Baskar K and Ignacimuthu S. („Ovicidal

activity of Couroupita guianensis

against cotton bollworm Helicoverpa

armigera (Hubner) (Lepidoptera:

Noctuidae)‰, Arc Phytopathol Plant

Prot, 2013), 46(13), 1571ă1579.

. Bergman J, Lindstrom J, Tilstam U.

„The structure and properties of some

indolic constituents in Couroupita

guianensis aubl‰, Tetrahedron, (1985),

, 2879-2881.

. Chandan BK, Saxena AK, Shukla S,

Sharma N, Gupta DK, Suri KA, Suri J,

Bhadauria M, and Singh B.

Hepatoprotective potential of Aloe

barbadensis mill against carbon

tetrachloride induced hepatotoxicity.

Journal of Ethnopharmacology 2007.

: 560ă6.

. Ellman GL. Tissue sulfhydryl groups.

Archives of Biochemistry and

Biophysics 1959.82, 70ă77.

. Elumalai A, Eswaraiah MC, Koppula

Naresh K, Kumar R, Meruva A,

Vidhyulatha C. („Antidiarrhoeal activity

of Couroupita guianensis leaves on

castor oil induced diarrhoea in albino

rats‰, Int J Pharmacol-Research Gate,

,3(2), 42-44.

. Elumalai A, Naresh V, Eswaraiah MC,

Narendar P, and Kumar R. „Evaluation

of antiulcer activity of Couroupita

guianensis leaves‰, Asian J Pharm

Tech, (2012), 2(2), 64ă66.

. Geetha M, Saluja AK, Shankar MB and

Mehta RS. „Analgesic and antiinflammatory activity of Couroupita

guianensis‰, J Nat Remed, (2004), 4,


. Geetha M, Shankar MB, Mehta RS, &

Saluja AK. „Anti-fertility activity of

Artabotrys odoratissimus Roxb. and

Couroupita guianensis‰, J Nat Rem,

(2005), 5, 121ă125.

. Hewawasam RP, Jayatilaka KAPW,

Pathirana C, Mudduwa LKB. Protective

effect of Asteracantha longifolia

extracts mouse liver injury induced by

carbon tetrachloride and paracetamol.

J Pharm Pharmacol 2003; 55: 1413-

. Jaishree V, Badami S. Antioxidant and

hepatoprotective effect of swertiamarin

from Enicostemma axillare against Dgalactosamine induced acute liver

damage in rats. Journal of

Ethnopharmacology 2010.130, 103ă

. Kind PR and King EJ. Estimation of

plasma phosphatase by determination

of hydrolysed phenol with antipyrine.

Journal of Clinical Pathology 1954.7,


. Kokate CK. Practical Pharmacognosy,

New Delli: Vallabh prakashan;


. Kornberg A. Lactic dehydrgenase of

muscle. In: Colowick, S.P.,

Kaplan,N.O., (Methods in Enzymology,

vol-1, Academic press, New York,


. Lee CP, Shih PH, Hsu CL, Yen GC.

Hepatoprotection of tea seed oil

(Camellia oleifera Abel.) against CCl4 -

induced oxidative damage in rats. Food

and Chemical Toxicology 2007. 45,


. Li CC, Hsiang CY, Wu SL, Ho TY.

Identification of novel mechanisms of

silymarin on the carbon tetrachlorideinduced liver fibrosis in mice by

nuclear factor-kappaB bioluminescent

imaging-guided transcriptomic analysis.

Food and Chemical Toxicology 2012.

, 1568ă1575.

. Lingathurai S, Vendan SE, Paulraj MG

and Ignacimuthu S. „Antifeedant and

larvicidal activities of Acalypha

fruticosa Forssk (Euphorbiaceae)

against Plutella xylostella L.

(Lepidoptera: Yponomeutidae) larvae‰,

J King Saud University Sci. (2011),

(1), 11ă16.

. Liu JR. Edamatsu H, Kabuto and Mori

A. Antioxidant action of Guilingji in the

brain of rats with FeCl3 induced

epilepsy. Free Radical Biology and

Medicine 1990. 9: 451ă54.

. Lowry OH, Rosebrough NJ, Farr AL,

Randal RJ. Protein measurement with

the folin phenol reagent. Journal of

Biological Chemistry, 1951.193: 265ă

. Malloy HT, Evelyn KA. The

determination of bilirubin with the

photometric colorimeter. Journal of

Biological Chemistry. 1937. 119, 481-

. Mihailovic V, Mihailovic M, Uskokovic

A, Arambasic J, Misic D, Stankovic

V,Katanic J, ladenovic M, Solujic S,

Matic S. Hepatoprotective effects of

Gentiana asclepiadea L. extracts

against carbon tetrachloride induced

liver injury in rats. Food and Chemical

Toxicology 2012. 52C, 83ă90.

. Mohandas J, Marshall JJ, Duggin GG,

Horvath JS, Tiller D. Differential

distribution of glutathione and

glutathione related enzymes in rabbit

kidney: possible interactions in

analgesic neuropathy. Cancer

Research, 1984.44: 5086ă5091.

. Mourelle M, Muriel P, Favari L, Franco

T. Prevention of CCl4-induced liver

cirrhosis by silymarin. Fundamental &

Clinical Pharmacology 1989. 3, 183ă

. Pinheiro MM, Bessa SO, Fingolo CE,

Kuster RM, Matheus ME, Menezes FS,

Fernandez PD. „Antinociceptive activity

of fractions from Couroupita guianensis

Aubl. LeavesÊ, J Ethnopharmacology,

(2010), 127 (2), 407-413.

. Pradhan D, Panda PK, and Tripathy G.

„Evaluation of Immunomodulatory

activity of the methanolic extract of

Couroupita guianensis flowers in rats‰,

Nat Prod Rad, (2009), 8(1), 37-42.

. Premanathan M, Radhakrishnan S,

and Kulangiappar K. „Antioxidant &

anticancer activities of isatin (1Hindole-2, 3-Dione), isolated from the

flowers of Couroupita guianensis

(November)‰, Indian J Med Res,

(2012), 136, 822ă826.

. Rai S, Wahile A, Mukherjee K, Saha

BP, Mukherjee PK. Antioxidant activity

of Nelumbo nucifera (sacred lotus)

seeds. Journal of ethnopharmacology.


. Raja S, Nazeer Ahamed KFH, Kumar

V, Kakali M, Bandyopadhyay A., Pulok

K. Mukherjee. Antioxidant effect of

Cytisus scoparius against carbon

tetrachloride treated liver injury in rats.

Journal of Ethnopharmacology 2007.

, 41ă47.

. Ramalakshmi C, Ranjitsingh AJA,

Kalirajan K, Kalirajan A, Athinarayanan

G and Mariselvam R „A preliminary

screening of the medicinal plant

Couroupita guianensis for its

antimicrobial potential against clinical

and fish-borne pathogens‰, Elixir Appl

Biol, (2013). 57, 14055ă14057.

. Regina V, and Rajan KMU,

„Phytochemical analysis, antioxidant

and antimicrobial studies of fruit rind of

Couroupita guianensis‰. Int J Curr Sci,

(2012), 221, 262ă267.

. Reitman S, Frankel SA. Colourimetric

method for the determination of serum

oxaloacetatic and glutamic pyruvic

transaminases. American Journal of

Clinical Pathology 1957. 28, 56ă63.

. Satyavati GV, Raina MK, Sharma M,

„Medicinal plants of India‰; Indian

Council of Medical Research,

Cambridge printing Works: New Delhi.

(1976),1, 286.

. Shah GN, Shete SA, Patil VS, Patil KD

and Killedar SG. „Standardization and

antibacterial activity of Couroupita

guianensis fruit pulp extract‰. Int J

Pharmacog Photochem Res,

(2012),4(4), 1ă5.

. Umachigi SP, Jayaveera KN, Ashok

CK, Kumar GS. „Antimicrobial, wound

healing and antioxidant potential of

Couroupita guianensis in rats‰.

Pharmacology online, (2007), 281(10),


. Usman MRM, Somani RP, Mohammed

A, Mohammed U. „Evaluation of

antipyretic activity of Anthocephalus

cadamba roxb. leaves extractsÊ. Res J

Pharm Biol Chem Sci, (2012).3(1),


. Vinod HG, Mahendra AG, Shaijesh

SW, Vishal SD, Archana RJ.

„Neuropharmacological evaluation of

the methanolic extract of Couroupita

guianensis flower in mice‰. Int J Pharm

Phytopharmacol Res. (2012), 1(5),


. Vinod HG, Shaijesh SW, Vishal SD,

and Archana RJ. „Anxiolytic effect of

Couroupita guianensis flower extracts

in mice‰, Int J Pharmacy Biol Sci,

(2013), 4(2), 420ă426.

. Wang L, Ran Q, Li DH, Yao HQ, Zhang

YH, Yuan ST, et al. Synthesis and anti

tumor activity of 14-O-derivatives of

natural oridonin. Chin J Nat Med 2011;

(3): 194-198.

. Wankhede SS, Gambhire M, Juvekar

A, and Marg NP. „Couroupita

guianensis. Evaluation of its

antidepressant activity in mice‰,

Pharmacologyonline, (2009), 2, 999-

. Wendel A. Glutathione peroxidase.

Methods in Enzymology 1981.77: 325ă

. Zeashan H, Amresh G, Singh S, Rao

ChV. Hepatoprotective activity of

Amaranthus spinosus in experimental

animals. Food Chem Toxicol 2008; 46:





How to Cite

Raja Sundararajan, Ravindranadh Koduru. Hepatoprotective and antioxidant effects of “Couroupita guianensis” on carbon tetra chloride - induced liver damage. ijp [Internet]. 2015 Sep. 30 [cited 2024 Jun. 23];7(3):281-9. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/416



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