Determination, identification of bioactive compounds extracts from yellow banana peels and used in vitro as antimicrobial
Keywords:
Banana peels, Total phenolic, Flavonoids, GC-MS Chromatography, antimicrobialAbstract
In this study, bioactive compounds of six solvent different extracts, from the dry yellow banana peels were present. Methanol was the best solvent extraction .The yield extract , total phenolic content and flavonoids concentration were 1.96 g, 81.89 mg G.A./g and 55.44mg RU/g from extract . The FT-IR spectra were recorded pronounced peaks belonging to the vibration of 3361 cm-1, 1662 cm-1and 1231 cm-1 in the spectra corresponds to the characterized peaks of flavonoids. GC-Ms chromatography clearly show the presence 17 component This analysis revealed that methanolic extract contain Pyrogallol (22.24%), Cis-9-Hexadecenal (21.20%) , Pentadecanoic acid (18.81%), Benzoic acid (16.04%) , Octadecanoic acid (6.18%) and Cis-9-Hexadecenoic acid (4.40%) . 300 mg / ml concentration from methanolic extract had broad inhibitory spectrum against gram positive and negative bacteria , yeast and molds .
References
. Jin D & Mumper R J, Plant phenolics
Extraction, analysis and their
antioxidant and anticancer properties,
Molecules,15 (2010) 7313.
. Soto-Vaca A, Losso J N, Xu Z &
Finley J W, Evolution of phenolic
compounds from color and flavor
problems to health benefits, J. Agric.
Food Chem,60 (2012) 6658.
. Ferreira O & Pinho S P, Solubility of
flavonoids in pure solvents, Ind. Eng.
Chem. Res, 51(2012) 6586.
. Larrauri J A, Ruperez P & Sauracalixto F, New approaches in the
preparation of high dietary fiber from
fruit by-products, Trends in Food
Science & Technology, 29 (1999) 729.
. Rodriguez de Sotillo D, Hadley M &
Holm E T, Potato peel waste stability
and antioxidant activity of freeze dried
extract, J. Food Sci, 59 (1994) 1031.
. Wolfe K, Xianzhong W U & Liu R H,
Antioxidant activity of apple peels, J.
Agric. Food Chem, 51(2003) 609.
. Emaga TH, Andrianaivo R H,
Wathelet B, Tchango J T & Paquot
M, Effects of the stage of maturation
and varieties on the chemical
composition of banana and plantain
peels, Food Chem, 103 (2007) 590.
. Baysal T, Ersus S & Starmans D A J,
Super critical CO2 extraction of β-
carotene and lycopene from tomato
paste waste, J. Agric. Food Chem, 48
(2000) 5507.
. Subaigo A, Morita N & Sawada S,
Carotenoids and their fatty acid esters
in banana peel, J. Nutr. Sci. Vitaminol,
(1996) 553 .
. Stankoviý M S, Total phenolic content ,
flavonoid concentration and antioxidant
activity of Marrubium peregrinum L.
extract , Kragujevac J. Sci, 33 (2010)
.
. Eghdami A & Sadeghi F,
Determination of total phenolic and
flavonoids contents in methanolic and
aqueous extract of Achillea millfolium ,
J. Org. Chem, 2(2010) 81.
. Krithika R, Lavanya P & Sowmya L,
Study of Antimicrobial and Antioxidant
Properties of Yellow Banana Peel
(Musa sapientum) to use it as a natural
food preservative, Int. J. Med. Res,1:
(2010) 338.
. Harbone J B, Phytochemical method
nd ed., Chapman, Hall, New York,
USA. (1984).
. Al ăKhazaraji SM, Biopharmacological
study of Artemisia herba M Sc Thesis,
College of pharmacy, Baghdad
university, Baghdad city, Iraq (1991).
. Temidayo AR, Extraction and isolation
of flavonoids present in the methanolic
extract of leaves of Acanthospermum
Hispidium Dc, Glob. J. Med. Plant Res,
(2012) 111.
. Singleton V L, Orthofer R & LamuelaRaventos R M, Analysis of total
phenols and other oxidation substrates
and antioxidants by means of FolinCiocalteureagent, Method. Enzymol,
(1999) 152.
. Quettier D C, Gressier B, Vasseur J,
Dine T, Brunet C, Luyckx MC, Cayin
JC, Bailleul F& Trotin F, Phenolic
compounds and antioxidant activities of
buckwheat (Fagopyrum esculentum
Moench) hulls and flour, J.
Ethnopharmacol,72 (2000) 35.
. Heneczkowski M, Kopacz M, Nowak
D & Kuzniar A, Infr red spectrum
analysis of some flavonoids , ACTA
Pol. Pharm,58 (2001)415.
. Zadernowski R, Naczk M & NowakPolakowska H, Phenolic acids of
borage (Borago officinalis L) and
evening primrose (Oenothera biennis
L.), Journal of the American Oil
ChemistÊs Society, 79 (2002) 335.
. Tagg J R, & McGiven A R, Assay
System for Bacteriocins. Appl.
Microbiol, 21 (1979) 943.
. Nene Y L & Thapliyal P N, Fungicides
in plant disease control, 3rd ed.
International Science Publisher,
Cambridge CB21 6AZ,United Kingdom,
(1993).
. Mohsen M S & Ammar S M A, Total
phenolic contents and antioxidant
activity of corn tassel extracts, Food
Chem,112 (2008) 595.
. Min G & Chun-Zhao L, Comparison of
techniques for the extraction of
flavonoids from cultured cells of
Saussurea medusa Maxim, World J
Microb Biot, 21(2005)1461.
. Jain Kassim M, Hazwan Hussin M,
Achmad A, Hazwani Dahon N, Kim
Suan T & Safley Hamdan H.
Determination of total phenol
condensed tannin and flavonoid
contents and antioxidant activity of
Uncaria gambir extracts, Majalah
Farmasi Indonesia, 22 (2011) 50.
. Brull S & Coote P, Preservative
agents in foods: mode of action and
microbial resistance mechanisms,
International journal of Food
Microbiology , 50 (1999) 1.
. Sangeetha J & Vijayalakshmi K,
Determination of bioactive components
of ethyl acetate fraction of Punica
granatum rind extract, International
Journal of Pharmaceutical Sciences
and Drug Research , 3(2): 116-122
. Uma B, Prabhakar K, Rajendran S &
Lakshmi Sarayu Y, Studies on GC/MS
pectroscopic Analysis of some
Bioactive Antimicrobial Compounds
from Cinnamomum zeylanicum ,
Journal of Medicinal Plants, 8(31)
(2009) 125.
. Lin J, Opoku A R & Geheeb-Keller M.
Preliminary screening of some
traditional Zulu medicinal plants for
anti-inflammatory and anti-microbial
activities, J. Ethnopharmacol, 68
(1999) 267.
. Mohammedi Z & Atik F, Fungitoxic
effect of natural extracts on mycelia
growth, spore germination and aflatoxin
B1 production of Aspergillus flavus,
Aust. J. crop sci. 7 (2013) 293.