Standardization of DPPH, ABTS and FRAP assays with six reference compounds for estimating antioxidant capacity of the tomato extracts using an ultrasound assisted extraction

Authors

  • Phitchan Sricharoen Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
  • Suchila Techawongstein Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002,Thailand.
  • Devanand Luthria U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Food Composition and Methods Development Laboratory, Bldg. 161, BARC(E), 10300 Baltimore Ave, Beltsville, MD 20705 Thailand.
  • Saksit Chanthai Associate Professor, Khon Kaen University, Faculty of Science, Department of Chemistry Thailand.

Keywords:

Antioxidant capacity, Total phenolic, Ascorbic acid, Ultrasonication extraction

Abstract

Comparative calibrating of the antioxidant power of standard compounds, including gallic acid, catechin, Trolox, α-tocopherol, ascorbic acid and BHT were assessed by DPPH, ABTS and FRAP assays to determine total antioxidant activity of ten varieties of tomato extracts using ultrasound assisted extraction. Similar trends in their scavenging activities were found, resulted in the following rank; gallic acid > catechin > trolox > α-tocopherol > ascorbic acid > BHT. Both lipophilic and hydrophilic parts of the extracts were given in these assays in association with using hexane and 50% (v/v) methanol as their efficient extraction solvents, respectively. Their averaged values of the ascorbic acid equivalent antioxidant capacities (AEAC, µmol ascorbic acid/100g DW) were relatively confined in the same ranges of 1341.8-1834.5, 1334.2-2194.8 and 930.7-1734.7 for DPPH, ABTS and FRAP, respectively. In addition, both contents of total phenolics and ascorbic acid known as major deals of antioxidants were also determined, indicating highly existing constituents of the natural antioxidants.

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Published

31-12-2014

How to Cite

1.
Phitchan Sricharoen, Suchila Techawongstein, Devanand Luthria, Saksit Chanthai. Standardization of DPPH, ABTS and FRAP assays with six reference compounds for estimating antioxidant capacity of the tomato extracts using an ultrasound assisted extraction. ijp [Internet]. 2014 Dec. 31 [cited 2024 Nov. 24];6(4):608-16. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/377

Issue

Vol. 6 No. 4 (2014): Oct-Dec

Section

Original Research Articles