Antimicrobial and anti oxidant activities of an Endophytic fungi Isolated from an endemic medicinal plant Pterocarpus santalinus

Authors

  • Nagam venkateswarlu s v university India.
  • Tartte Vijaya Department of Botany, Sri Venkateswara University, Tirupati-517 502, A.P, India.
  • Netala Vasudeva Reddy Department of Botany, Sri Venkateswara University, Tirupati-517 502, A.P, India.
  • K. K. Shama Department of Microbiology, Sri Venkateswara institute of medical science, Tirupati-517 502, A.P, India.

Keywords:

Endophytic fungi, molecular characterization, antimicrobial activity, antioxidant activity

Abstract

Endophytic fungi are intriguing microorganisms live inside the healthy plant tissues. they are quite diverse in nature and have enormous potential for production of important secondary metabolites of biomedical, pharmaceutical and clinical importance. In the present work we have isolated an endophytic fungi from the leaves of Pterocarpus santalinus an endemic medicinal plant of Eastern ghats, Tirumala hills, India. Based on the molecular characterization by 18S rRNA analysis the fungi was identified as Xylaria spp. ENT2 (Accession No. KF493856.1). the crude extract of Xylaria sp. was evaluated for antioxidant and antimicrobial activities. Among the other extracts tested methanolic extract showed highest activity against all the bacterial and fungal pathogens with a minimum bactericidal concentration (MBC) of about 30μg/mL against Staphylococcus aureus and a Minimum Fungicidal Concentration (MFC) of about 50μg/mL against Candida albicans. Similarly methanolic extract proved to be potential natural antioxidant with 56.26±0.08 radical scavenging activity (RSA).

References

. Xiaoli Liu, Mingsheng Dong, Xiaohong

Chen, Mei Jiang, Xin Lv, Guijun Yan.

Antioxidant activity and phenolics of an

endophytic Xylaria sp. from Ginkgo

biloba. Food Chemistry 2007;105: 548ă

. Sogra Fathima Musavi and Raj Mohan

Balakrishnan A Study on the

Antimicrobial Potentials of an

Endophytic Fungus Fusarium

oxysporum NFX 06, Journal of Medical

and Bioengineering2014; 3 (3):161-

. Newman DJ, Cragg MG. „Natural

products as source of new drugs over

the last 25 years,‰ J. Nat. Prod.,

;70: 461-477,.

. Demain AL, Sanchez S. „Microbial

drug discovery: 80 years of progress,‰

J. Antibiot., 2009;62: 5-16.

. Kirtikar & Basu. Indian Medicinal

Plants. IInd Edn., Vol I, 1987; p.826-

. Warrier PK, Nambiar VPK, Raman

Kutty C. Indian Medicinal Plants, Orient

Longman Ltd., 1995; 5: 384-387.

. Petrini O, Fungal endophytes of tree

leaves. In: Andrews JH, Hirano SS,

eds. Microbial Ecology of Leaves. New

York: Spring Verlag: 1991: 179-197.

. Strobel G, Daisy B. Bioprospecting for

Microbial Endophytes and Their

Natural Products. Microbiol. Mol. Biol.

Rev., 2003; 67: 491ă502.

. Aly AH, Debbab A, Kjer J, Proksch P.

Fungal endophytes from higher plants:

a prolific source of phytochemicals and

other bioactive natural

products. Fungal Divers. 2010;

(1):1ă16.

. Yu H, Zhang L, Li L. et al., „Recent

developments and future prospects of

antimicrobial metabolites produced by

endophytes,‰ Microbiological Research,

;165(6): 437-449.

. Song JH. „WhatÊs new on the

antimicrobial horizon?‰ International

Journal of Antimicrobial Agents,

;32(4): S207ăS213.

. Dreyfuss MM, Chapela IH. „Potential

of fungi in the discovery of novel, low

molecular weight pharmaceuticals,‰

The Discovery of Natural Products with

Therapeutic Potential, V. P. Gullo, Ed.,

Boston: Butterworth- Heinemann,

;7:49-80.

. Arnold AE, Meijia LC, Kyllo D, Rojas E

I, Maynard Z. et al., „Fungal

endophytes limit pathogen damage in a

tropical tree,‰ in Proc. National

Academy of Science, 2003;100 (26):

-15654.

. Zhang HH, Song YC, Tan RX.

„Biology and chemistry of endophytes,‰

Nat. Prod. Rep., 2006;23: 753-771.

. Narayan CP, Won KK, Sung KW,

Myung SP, Seung HY. Plant Pathol. J.,

;23( 4) :287-294.

. Wang F W, Jiao RH, Cheng AB, Tan S

H, Song YC. „Antimicrobial potentials

of endophytic fungi residing in quercus

variabilis and brefeldin a obtained from

cladosporium sp.,‰ World J Microbiol

Biotechnol, 2006; 23: 79-83.

. Gangadevi V, Muthumary J. „Taxol, an

anticancer drug produced by an

endophytic fungus bartalinia

robillardoides Tassi, isolated from a

medicinal plant, aegle marmelos correa

ex roxb,‰ World Journal of Microbiology

and Biotechnology, 2008; 24: 717-724.

. Duan XJ, Zhang WW, Li XM, Wang

BG. Evaluation of antioxidant property

of extract and fractions obtained from a

red alga, Polysiphonia urceolata. Food

Chemistry, 2006: 95, 37ă43.

. Shon MY, Kim TH, Sung NJ.

Antioxidants and free radical

scavenging activity of Phellinus baumii

(Phellinus of Hymenochaetoceae)

extracts. Food Chemistry, 2003;82:

ă597.

. Bills GF, Polishook JD. „Microfungi

from carpinus caroliniana can,‰ J. Bot.

vol. 1991;69: 1477-1482.

. Powthong P, Jantrapanukorn B,

Thongmee A, Suntornthiticharoen P.

„Evaluation of endophytic fungi extracts

for their antimicrobial activity from

sesbania grandiflora (L.) pers,‰ Int J

Pharm Biomed Res, 2012; 3(2)P: 132-

. Rios JL, Recio MC. „Medicinal plants

and antimicrobial activity,‰ J.

Ethnopharmacol, 2005; 100:80-84.

. Maria GL, Sridhar KR, Raviraja NS.

J Agri Tech, 2005; 1: 67-80.

Downloads

Published

31-12-2014

How to Cite

1.
Nagam venkateswarlu, Tartte Vijaya, Netala Vasudeva Reddy, K. K. Shama. Antimicrobial and anti oxidant activities of an Endophytic fungi Isolated from an endemic medicinal plant Pterocarpus santalinus. ijp [Internet]. 2014 Dec. 31 [cited 2024 Nov. 13];6(4):523-8. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/366

Issue

Section

Original Research Articles