Evaluation of hypoglycemic properties and fertility effect of Piper sarmantosum Roxb. aqueous leaf extract in streptozotocin induced diabetic mice


  • Ampa - Luangpirom Biology Department Associate Professor, Khon Kaen University Thailand.
  • Watchara Kourchampa Department of Biology, Faculty of Science, Khon Kaen University Thailand
  • Pichet Somsapt Depaertment of Radiology, Faculty of Medicine, Khon Kaen University Thailand


hyperglycemic properties, fertility effect, insulin, Piper sarmentosum Roxb


Male mice of ICR strain (6-7 week old, 30-40 g) were used for evaluation of the hypoglycemic and fertility effect of Piper sarmentosum Roxb. leaf extract. Diabetes was induced by intraperitoneal (i.p) injection of streptozotocin (STZ) (6.0 mg/100gBW) and glibenclamide (reference drug) 1 mg/ 100gBW and Piper sarmentosum Roxb. leaf extract (PS) at 60 and 100 mg/100gBW were orally administered (per os) for 21 days. The hypoglycemic activity of PS was 73.04 and 120.96% of the glibenclamide. Meanwhile, the blood insulin level also significantly increased by 8.25 and 50.53 % of the diabetic control. The concordant results showed that diabetic pancreatic islets were impaired and improved after extract treatment. However, the fertility status test showed that the seminal quality and blood testosterone of diabetic mice decreased significantly (P< 0.05) as compared to the normal mice and significantly increased when compared to the diabetic control after long-term treatment. In conclusion, Piper sarmentosum Roxb. aqueous leaf extract at doses of 60 and 100 mg/100 gBW revealed hyperglycemic properties in diabetic mice. They also improved both pancreatic islet function and fertility status of diabetic mice after 21 days of extract treatment.


. Tiwari AK, Roa M. Diabetes mellitus and multiple therapeutic approaches of phytochemicals: present status and future prospects, Curr Sci, 2002; 83: 30-38.

. Baynes JW, Thorpe SR. 1999. Role of oxidative stress in diabetic complications: A new perspective on an old paradigm, Amer Diabetes Assoc, 1999; 48: 1-9.

. Vijayalingam S, Parthiban A, Shanmugasundaram KR, Mohan V. Abnormal antioxidant status in impaired glucose tolerance and non-insulin-dependent diabetes mellitus, Diabetic Med, 1996; 13: 715-719.

. Atkinson MA, Maclaren NK. The pathogenesis of insulin-dependent diabetes mellitus (review), N Eng J Med, 1994; 331: 1428-1436.

. Khaki A, Nouri M, Fathiazad F, Ahmadi-Ashtiani HR, Rastgar H, Rezazadeh Sh. Protective effect of quercetin on spermatogenesis in streptozotocin-induced diabetic rats, Int Med Pl, 2009; 8(supplement): 57-64.

. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas, Physiol Res, 2001; 50(6): 537-546.

. Subramaniam V, Adenan IM, Ahmad AR, Sahdan R. Natural antioxidant Piper sarmentosum and Morinda ellpitc, Mal J Nutr, 2003; 9: 41-51.

. Hafizah AH, Zaiton Z, Zulkhairi A, Ilham AM, Anita MMNN, Zaleha AM. Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide, J Zhejiang Uni Sci Biomed Biotech, 2010; 5: 357- 365.

. Priscilla DH, Roy D, Suresh A, Kumar V, Thirumurugan K. Naringenin inhibits α-glucosidase activity: A promising strategy for the regulation of postprandial hyperglycemia in high fat diet fed streptozotocin induced diabetic rats, Chemico-Biol Interact, 2014; 210: 77-85.

. Rahman NA, Noor KM, Hlaing KPP, Suhaimi FH, Kutty MK, Sinor MZ. Piper Sarmentosum influences the oxidative stress involved in experimental diabetic rats, Internet J Herb Pl Med, 2011; 1: 1-9.

. Sexton WJ, Jarow JP. Effect of diabetes mellitus upon male reproductive function, Urol, 1997; 49: 508-513.

. Peungvicha P, Suwan S. Hypoglycemic effect of the water extract of Piper sarmentosum in rats, J Ethnopharmacol, 1989; 60: 27-32.

Ali Madhi MA, Chandra A, Singh RK, Shukla S, Mishra LC, Ahmad S. 2003. Effect of herbal hypoglycemic agents on oxidative stress and antioxidant status in diabetic rats, Indian J Clin Biochem, 2003; 18(2): 8-15.

Latha M, Pari L. Effect of an aqueous extract of Scoporia dulcis on blood glucose, plasma insulin and some polyol pathway enzymes in experimental rat diabetes, Brazillian J Med Biol Res, 2004;37: 577-586.

. Luangpirom A, Junaimuang T, Kourchampa W, Somsapt P, Sritrakool O. Protective effect of pomegranate (Punica granatum Linn) juice against hepatotoxicity induced by ethanol in mice, ABAH BioFlux, 2013; 8: 89-93.

. Zar JH. Biostatistical analysis, International edition, 4th ed. Prentice Hall International, Inc., New Jersey, 1999, p 662.

. World Health Organization. WHO list of essential medicines, 15th edition, World Health Organization, 2007, p21.

. Ashcroft FM, Ashcroft SJH. The sulfonylurea receptor, Biochim Biophys Acta, 1992; 1175:45-59.

. Eliasson L, Renstrom E, Ammala C, Berggran PO, Bertorello AM, Bokvist K, Chibalin A, Deeney JT, Flatt IR, Gabel J, Gromacia J, Larsson O, Linstrom P, Rhodes C, Rossman P. PKC-dependent stimulation of β-cells, Science, 1996; 271: 813-815.

. Jennings PE, Belch JJF. Free radical scavenging activity of sulfonylureas: A clinical assessment of the effect of gliclazide, Metabolism, 2000; 49(2): 23-26.

. Krisanapun C, Womgkrajang Y, Temsiririrkkul R, Phonrnchirasilp S, Peungvicha P. In vitro evaluation of anti-diabetic potential of Piper sarmentosum Roxb. extract, The

FASEB Journal, 2012; 26: 686.7.

. Doreswamy K, Shrilatha B, Rajeshkummar T, Muralidhara. Nickel induced oxidative stress in testis of mice, J Androl, 2004; 25:996-1003.

. Shrilatha B, Muralidhara. Early oxidative stress in testis and epididymis sperm in streptozotocin-induced diabetic mice: Its progession and genotoxic consequences, Repro Toxicol, 2007; 23:578-587.

. Keskes-Ammar L, Feki-Chanroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, Zghal K, Fki H, DAmak J, Bahloul A. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men, Arch Androl, 2003; 49: 83-94.

. Kefer JC, Agarwal A, Sabanegh F. Role of antioxidants in the treatment of male infertity, Int J Urol, 2009; 16:447-457.

. Benitez A, Perez-Diaz J. Effect of steptozotocin-diabetes and insulin treatment on regulation of Leydig cell function in the rat, Horm Metab Res, 1985; 17:3-7.

. Johnson HM, Everitt BJ. Essential reproduction, Blackwell Science Pty Ltd.1995, Australia.




How to Cite

Ampa - Luangpirom, Watchara Kourchampa, Pichet Somsapt. Evaluation of hypoglycemic properties and fertility effect of Piper sarmantosum Roxb. aqueous leaf extract in streptozotocin induced diabetic mice. ijp [Internet]. 2014 Sep. 30 [cited 2024 Jul. 15];6(3):448-54. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/358



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