Validated HPTLC method for standardization of an ayurvedic powdered formulation-Panchsakar churna

Authors

  • Puspendra Kumar KIET School of Pharmacy, KIET Group of Institutions, Ghaziabad, 201206. Uttar Pradesh, India.
  • Shivesh Jha Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi-835215. Jharkhand, India.
  • Durgawati Patel Department of Chemistry, Government Nagarjuna Post Graduate College of Science, Raipur, 492010. Chhattisgarh, India.
  • Sanjar Alam KIET School of Pharmacy, KIET Group of Institutions, Ghaziabad, 201206. Uttar Pradesh, India. India

Keywords:

Panchsakar Churna, HPTLC, Standardization, Sennoside, Gallic acid

Abstract

Objective: Panchsakar Churna is a marketed ayurvedic powdered formulation containing Cassia angustifolia and Terminalia chebula as the main ingredients. Present study aims to standardize Panchsakar Churna based upon chromatographic and spectral studies. Methods: This study developed an efficient and reliable high performance thin layer chromatographic method for quantification of Sennoside B and Gallic acid in Panchsakar Churna. The developed method was validated for linearity, accuracy, precision, sensitivity and purity. Results: Linearity of peak area was tested in the range 0.310-3.100µg/spot (r=0.9835) and 0.200-2.000 µg/spot (r=0.9929), Accuracy was assessed with recovery and the recoveries were 99.909-100.330 % and 99.690-100.562 % (n=3 for each sample), Precision were 0.257-2.580 and 0.425-2.201, sensitivity was determined with respect to limit of detection and limit of quantification, limit of detection were 73.7634 ng/spot and 50.1556 ng/spot, while limit of quantification were 245.8781ng/spot and 167.1853ng/spot, purity of the spot were 0.9984-0.9999 (r) and 0.9977-0.9998 (r) respectively for Sennoside B and Gallic acid. Conclusion: Sennoside B and Gallic acid can be used as possible marker compound for standardization of Panchsakar Churna.

References

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Published

30-09-2014

How to Cite

1.
Puspendra Kumar, Shivesh Jha, Durgawati Patel, Sanjar Alam. Validated HPTLC method for standardization of an ayurvedic powdered formulation-Panchsakar churna. ijp [Internet]. 2014 Sep. 30 [cited 2024 Feb. 28];6(3):417-20. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/354

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Original Research Articles