Effect of aqueous extract of Ficus platyphylla on female Wistar rats with estradiol valerate-induced polycystic ovarian syndrome

Authors

  • Chinenye Jane Ugwah-Oguejiofor Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
  • Shaibu Oricha Bello Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
  • Raymond U Okolo Department of Anatomy, College of health Sciences, Usmanu Danfodiyo University Sokoto, Nigeria.
  • Emmanuel U Etuk Department of Pharmacology, College of health Sciences, Usmanu Danfodiyo University Sokoto, Nigeria.
  • Michael Oguejiofor Ugwah Department of Pharmacy, Usmanu Danfodiyo University Teaching hospital, Sokoto, Nigeria
  • Vincent Ugochukwu Igbokwe Department of Physiology, College of health Sciences, Usmanu Danfodiyo University Sokoto, Nigeria
  • Mohammed Umar Department of Pathology, Usmanu Danfodiyo University Teaching hospital, Sokoto, Nigeria

Keywords:

Ficus platyphylla, polycystic ovarian syndrome, infertility, estradiol, Wistar rats

Abstract

The aim of the study was to investigate the effects of Ficus platyphylla on female Wistar rats with estradiol valerate (EV)-induced polycystic ovarian syndrome (PCOS). PCOS was induced in female Wistar rats with regular 4-5 day oestrus cycles via the intramuscular injection of 4 mg EV per rat in an oily solution. The rats were allowed 30 days to establish PCOS. Then, the animals were divided into five treatment groups. The positive control group received clomiphene citrate, the negative control group received distilled water and the other groups received 100, 200 and 400 mg/kg of the aqueous extract. All groups were dosed for 15 days, except the positive control group, which was dosed for 5 days. On the 16th day, the animals were sacrificed. Hormonal assays and histological studies were then conducted. An elevated Luteinizing hormone (LH)-to- Follicle stimulating hormone (FSH) ratio is typical in many women with PCOS. The extract-treated groups showed a lower LH/FSH ratio compared with both the positive and the negative control groups. The progesterone levels were higher in the extract-treated groups compared with the negative control group, indicating luteal phase repair. The influence of the extract on ovarian morphology in the EV-induced PCOS model showed a marked reversal of polycystic ovary. These data help validate the use of this extract in folk medicine for the treatment of infertility. Considering its apparent safety, this study paves the way for an efficacy study for its use in treatment of PCOS in humans.

References

Ruiz-Luna AC, Salazar S, Aspajo NJ, Rubio J, Gasco M, Gonzales GF. Lepidium meyenii (Maca) increases litter size in normal adult female mice. Reprod Biol Endocrinol. 2005; 3: 16.

Ogbe FMD, Eruogun OL, Uwagboe M. Plants used for female reproductive health care in Oredo local government area Nigeria. Sci Res Essay. 2009; 4: 120-130.

Jha U, Asad M, Asdaq SMB, Das AK, Prasad VS. Fertility inducing effect of aerial parts of Coccinia cordifolia L. in female rats. J Ethnopharmacol. 2010; 127: 561-564.

Chaturapanich G, Chaiyakul S, Verawatnapakul V, Pholpramool C. Effects of Kaempferia parviflora extracts on reproductive parameters and spermatic blood flow in male rats. Reprod. 2008; 136: 515-522.

Ugwah-Oguejiofor CJ, Bello SO, Okolo RU, Etuk EU, Ugwah OM, Igbokwe VU. Ficus platyphylla promotes fertility in female Rattus norvegicus Wistar strain: a preliminary study. Reprod Biol Endocrinol. 2011; 9: 145.

Tsilchorozidou T, Overton C, Conway GS. The pathophysiology of polycystic ovary syndrome. Clin Endocrinol. 2004; 60: 1–17.

Barbieri RL. Metformin for the treatment of polycystic ovary syndrome. Obstet gynaecol. 2003; 101: 785-793.

The Rotterdam ESHRE/ASRM sponsored PCOS consensus workshop group: Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Human Reprod. 2004; 19: 41-47.

Singh KB. Persistent estrus rat models of polycystic ovary disease: an update. Fertil Steril. 2005; 84: 1228-1234.

Lujan ME, Chizen DR, Pierson RA. Diagnostic criteria for polycystic ovary syndrome: pitfalls and controversies. J Obstet Gynaecol Can. 2008; 30: 671-679.

Sirmans SM, Pate KA. Epidemiology, diagnosis, and management of polycystic ovary syndrome. Clin Epidemiol. 2013; 6:1-13.

Brawer JR Munoz M, Farookhi R. Development of the polycystic ovarian condition (PCO) in the estradiol valerate-treated rat. Biol Reprod. 1986; 35: 647-655.

Adashi EY. Clomiphene citrate-initiated ovulation: a clinical update. Endocrinol. 1986; 4: 255-276.

Marantides D. Management of Polycystic ovarian syndrome. The nurse Practitioner. 1997; 22: 34-44.

Kousta E, White DM, Franks S. Modern use of clomiphene citrate in induction of ovulation. Human Reprod Update. 1997; 3: 359–365.

Wu CH, Winkel CA. The effect of therapy initiation day on clomiphene citrate therapy. Fertil Steril. 1989; 52: 564–568.

Chindo BA, Amos S, Odutola AA, Vongtau HO, Abbah J, Wambebe C, Gamaniel KS. Central nervous system activity of the methanol extract of Ficus platyphylla stem bark. J Ethnopharmacol. 2003; 85: 131–137.

Audu J. Medicinal herbs and their uses in Bauchi State. The Nigerian field. 1989; 54: 157–168.

Amos S, Binda L, Chindo B, Akah P, Abdulrahman M, Danmallam HU, Wambebe C, Gamaniel K. Evaluation of methanolic extract of Ficus platyphylla on gastrointestinal activity. Indian J Exp Biol. 2001; 39: 63–67.

Amos S, Chindo B, Edmond I, Akah P, Wambebe C, Gamaniel K. Antinociceptive and anti-inflammatory properties of Ficus platyphylla stem bark. J Herbs, Spices Med Plants. 2002; 9: 47–53.

Ugwah-Oguejiofor CJ, Bello SO, Etuk EU, Igbokwe VU, Ugwah OM, Okolo RU. Preliminary toxicity and phytochemical studies of the aqueous extract of Ficus platyphylla in female albino rats. Int Res J Pharm Pharmacol. 2011; 1: 86-92.

Organization for Economic Development (OECD): Principles of Good Laboratory Practice, in: hand Book of Good Laboratory Practice (GLP) TDR, PRD/GLP/01.2, 2008.

Kafali H, Iriadam M, Ozardal I, Demir N. Letrozole-Induced Polycystic ovaries in the rat: A new model for cystic ovarian disease. Arch Med Res. 2004; 35: 103–108.

Freireich EJ, Gehan E, Rall D, Schmidt L, Skipper H. Quantitative comparison of toxicity of anticancer agents in mouse, rat, dog, monkey and man. Cancer Chemother Rep.1966; 50: 219-244.

Baravalle C, Salvetti NR, Mira GA, Pezzone N, Orteaga HH. Microscopic characterization of follicular structures in Leotrozole-induced polycystic ovarian Syndrome in the rat. Arch med res. 2006; 37: 830-839.

Mahajan DK. Steroidogenesis in human polycystic ovary. Endocrinol Metabol Clin North Amer. 1988; 17: 751-769.

Ushiroyama T. Endocrinological actions of Unkei-to, a herbal medicine, and its clinical usefulness in anovulatory and/or infertile women. Reprod Med biol. 2003; 2: 45-61.

Lobo RA, Paul W, March CM, Granger L, Kletzky OA. Clomiphene and dexamethasone in women unresponsive to clomiphene alone. Obstet Gynaecol. 1982; 60: 497–501.

Parsanezhad ME, Alborzi S, Motazedian S, Omrani G. Use of dexamethasone in the treatment of clomiphene citrate resistance in patients with polycystic ovary syndrome and normal dehydroepiandrosterone sulfate levels: a prospective, double-blind, placebo- controlled trial. Fertil Steril. 2002; 78: 1001–1004.

Trott EA, Plouffe L Jr, Hansen K, Hines R, Brann DW, Mahesh VB. Ovulation induction in clomiphene-resistant anovulatory women with normal DHEAS levels: beneficial effects the addition of dexamethasone during the follicular phase. Fertil Steril. 1996; 66: 484–486.

Quandt LM, Hutz RJ. Induction by Estradiol-17β of Polycystic Ovaries in the Guinea Pig. Biol Reprod. 1993; 48: 1088-1094.

McKenna TJ. Pathogenesis and treatment of polycystic ovary syndrome. N Engl J Med. 1988; 318: 558-562.

Hemmings R, Farookhi R, Brawer JR. Pituitary and ovarian responses to luteinizing hormone releasing hormone in a rat with polycystic ovaries. Biol Reprod. 1983; 29: 239-248.

Koyama T, Ohara M, Ichimura M, Saito M. Effect of Japanese kampo medicine on hypothalamic-pituitary-ovarian function in women with ovarian insufficiency. Amer J Chinese Med. 1988; 16: 47–55.

McCarthy GF, Brawer JR. Induction of Stein-Leventhal-like polycystic ovaries (PCO) in the rat: a new model for cystic ovarian disease. Anat Rec. 1990; 228: 137-144.

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Published

30-09-2014

How to Cite

1.
Chinenye Jane Ugwah-Oguejiofor, Shaibu Oricha Bello, Raymond U Okolo, Emmanuel U Etuk, Michael Oguejiofor Ugwah, Vincent Ugochukwu Igbokwe, Mohammed Umar. Effect of aqueous extract of Ficus platyphylla on female Wistar rats with estradiol valerate-induced polycystic ovarian syndrome. ijp [Internet]. 2014 Sep. 30 [cited 2024 Oct. 3];6(3):405-11. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/352

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Original Research Articles