Antiepileptic activity of leaves of Leucas aspera.

Authors

  • Ramalingam Ramani Department:Pharmaceutical Chemistry, Associate Professor, Swami Ramananda Tirtha Institute of Pharmaceutical Sciences India.
  • Bindu Madhavi Boddupalli Swami Ramananda Tirtha Institute of Pharmaceutical Sciences, Nalgonda, Telangana, India. 508004 India.
  • Ramya Miryala Swami Ramananda Tirtha Institute of Pharmaceutical Sciences, Nalgonda, Telangana, India. 508004 India.
  • Ravinder Nath Anisetti Department of Pharmacy, University College of Technology, Osmania University, Hyderabad, Telangana, India. 500017 India.
  • Nagulu Malothu Swami Ramananda Tirtha Institute of Pharmaceutical Sciences, Nalgonda, Telangana, India. 508004 India.
  • Deepthi Balla University College of Science of Informatics, Mahatma Gandhi University, Nalgonda, Andhra Pradesh, India. 508001 India.

Keywords:

Leucas aspera, Malondialdehyde, Pentylenetetrazole

Abstract

Leucas aspera (LA) is a plant that has been used in folk medicine to treat asthma, fever, skin diseases and has several pharmacological activities. The antiepileptic property of LA was not yet been studied. Aim: The present study was aimed to investigate the antiepileptic activity of ethanolic extract obtained from leaves of LA on pentylenetetrazole (PTZ) kindling seizures in mice. Method: The ethanolic extract 200mg/kg and 400mg/kg were evaluated for the antiepileptic activity against PTZ (40mg/kg IP) induced seizure method. Diazepam was used as the standard drug. Antiepileptic activity was evaluated by observing seizure intensity, motor coordination, depression and oxidative stress by malondialdehyde (MDA) content. Results: Treatment with extract was found to be in dose dependant manner with significant prolonged onset time, decreased duration and intensity of seizure when compared with vehicle treated group. Extract has even protected the animal from loss of motor coordination and depression. Conclusion: From the results of present study it can be concluded that, the ethanolic extract of leaves of LA protected the animals from seizure effect induced by PTZ and attenuated the oxidative stress induced by PTZ without producing loss of motor coordination and depression.

References

Adriana DR, Thamiris BS, Gustavo S, Maite TS, Bruna F, Suzana CVF, Caroline D, Regina V, Joao APH, Adriana SC, Mirian S. Neuroprotective and anticonvulsant effects of organic and conventional purple grape juices on seizure in wistar rats induced by pentylenetetrazole. Neurochem Int. 2012; 60: 799-805.

Leker RR, Neufeld MY. Antiepileptic drugs as possible neuroprotectants in cerebral ischemia. Brain Res Rev. 2003; 42: 187-203.

Jianming G, Caifu X, Jinao D, Dawei Q, Yuping T, Yi Y. Anticonvulsant, antidepressant like activity of Abelmoschus manihot ethanol extract and its potential active components in vivo. Phytomedicine. 2011; 18: 1250-1254.

Madhava CK, Sivaji K, Tulasi RK. Flowering plants of chittor district. 1st ed. Tirupathi, India: Students offset printers; 2008: p. 277-278.

Hemendra SC, Sushil KS. A review of plants of genus leucas. J Pharmacog Phytother. 2011; 3(3): 13-26.

Mangathayaru K, lakshmikant J, Shyam SN, Swapna R, Fatima XG, Vasantha J. Antimicrobial activity of Leucas aspera flowers. Fitoterapia. 2005; 76: 752-754.

Rahman MS, Sadhu SK, Hasan CM. Preliminary antinociceptive, antioxidant and cytotoxic activities of Leucas aspera root. 2007; 78: 552-555.

Meghashri S, Vijay KH, Gopal S. Antioxidant properties of a novel flavonoid from leaves of Leucaas aspera. Food Chem. 2010; 122: 105-110.

Ramaraj T, Rajarajeswaran J. Protection of cadmium chloride induced DNA damage by Lamiaceae plants. Asian Pac J Trop Biomed. 2011; 391-394.

Atilla I, Ahmet G, Ferah A, Suat K, Mustafa I. Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazol-induced kindling in mice. Neuropharmacol. 2005; 49: 456-464.

Neeraj C, Krishna RVB, Ajudhia NK. Antiepileptic potential of flavonoids from the leaves of Anisomeles malabarica. J Ethanopharmacol. 2011; 135: 238-242.

Bhupinder S, Damanpreet S, Rajesh KG. Dual protective effect of Possiflora incarnate in epilepsy and associated post-ictal depression. J Ethanopharmacol. 2012; 139: 273-279.

Eun JS, Ji HJ, Yoon HC, Won KK, Kwang HK, Jae HB, Jau SH, Yukio Y, Hyoung CK. Role of oxidative stress in epileptic seizures. Neurochem Int. 2011; 59: 122-137.

Jogender M, Reeta KH, Pooja G, Yogendra KG. protective effect of curcumin against seizure and cognitive impairment in a pentylenetetrazole kindled epileptic rat model. Life Sci. 2010; 87: 596-603.

Marjan NA, Schwann SR, Farzaneh Z. Anticonvulsive effects of intra cerebro ventricular administration of rutin in rats. Prog Neuropsychopharmacol Biol Psychiatry. 2008; 32: 989–993.

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Published

30-09-2014

How to Cite

1.
Ramalingam Ramani, Bindu Madhavi Boddupalli, Ramya Miryala, Ravinder Nath Anisetti, Nagulu Malothu, Deepthi Balla. Antiepileptic activity of leaves of Leucas aspera. ijp [Internet]. 2014 Sep. 30 [cited 2024 Feb. 28];6(3):391-14. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/350

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Original Research Articles