Induction of caspase dependent apoptosis by α-Mangostin in breast cancer cells


  • Navya Atluri Department of Applied Microbiology, Sri PadmavatiMahilaVisvavidyalayam (S.P. Women’s University), Tirupati-517502, Andhra Pradesh, India.
  • Vasavi Thirumalanadhuni Department of Applied Microbiology, Sri PadmavatiMahilaVisvavidyalayam (S.P. Women’s University), Tirupati-517502, Andhra Pradesh, India.
  • Uma Maheswari devi Palempalli professor, dept. of applied microbiology, Department of Applied Microbiology, Sri PadmavatiMahilaVisvavidyalayam (S.P. Women’s University), Tirupati-517502, Andhra Pradesh, India.


α-Mangostin, Apoptosis, Extrinsic pathway, Caspase-3, DNA fragmentation


Apoptotic potential of α-Mangostin (98-99.99% HPLC purified)was evaluated in vitro using MDA-MB-231 human breast cancer cell line as a model system. The nucleosomal DNA gel electrophoresis was performed to delineate the mechanism of α-Mangostin treated MDA-MB-231 cells death. The dose-dependent oligonucleosomal fragmentation of DNA was observed in α-Mangostin treated cells. Flow cytometrywas performed to confirm the apoptotic potential of α-Mangostin (10µM) on MDA-MB-231 cells. The control cells showed only 1.85% of hypodiploid DNA and the percentage increased to 13.70% on exposure of cells to α-Mangostin. Caspase-3, a critical regulator of cell death, was detected in α-Mangostin treated MDA-MB-231 cells by Immunocytochemical analysis. These results demonstrated the expression of caspase-3 in MDA-MB-231 cells exposed to 10µM of α-Mangostin. The results suggest that α-Mangostin induced cell death was by the activation of cell death pathway particularly mediated by caspase-3.


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How to Cite

Navya Atluri, Vasavi Thirumalanadhuni, Uma Maheswari devi Palempalli. Induction of caspase dependent apoptosis by α-Mangostin in breast cancer cells. ijp [Internet]. 2014 Sep. 30 [cited 2024 Feb. 28];6(3):354-9. Available from:



Original Research Articles