Isolation of novel phytoconstituents from the stem part of Cleome gynandra Linn and their antimicrobial activity
Keywords:
Cleome gynandra Linn, Antibacterial and antifungal activities, phytoconstituents, Spectral interpretationAbstract
Dried and powdered stem of Cleome gynandra Linn were extracted with methanol and chloroform solvents. From the extract, four individual components were identified using UV-Visible Spectrophotometer, FT-IR, GC-Mass and NMR technique. The extracted components were isolated as pure form using Silicagel-G column chromatography. Identity of these four novel compounds such as β-amyrin, β -Amyrin-3-O- β-Glucopyranoside, Stigmasetrol and Sitosterol was confirmed by spectral interpretation. These compounds were tested for their antimicrobial activity and showed inhibition against all the tested bacterial and fungal cultures.
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