Isolation of novel phytoconstituents from the stem part of Cleome gynandra Linn and their antimicrobial activity


  • Ranjitha J CO2 Research and Green Technologies Centre, VIT University, Vellore-14 India.
  • Madonna Shalma CO2 Research and Green Technologies Centre, VIT University, Vellore-14 India
  • Michael Donatus CO2 Research and Green Technologies Centre, VIT University, Vellore-14 India.
  • Vijayalakshmi S CO2 Research and Green Technologies Centre, VIT University, Vellore-14 India.


Cleome gynandra Linn, Antibacterial and antifungal activities, phytoconstituents, Spectral interpretation


Dried and powdered stem of Cleome gynandra Linn were extracted with methanol and chloroform solvents. From the extract, four individual components were identified using UV-Visible Spectrophotometer, FT-IR, GC-Mass and NMR technique. The extracted components were isolated as pure form using Silicagel-G column chromatography. Identity of these four novel compounds such as β-amyrin, β -Amyrin-3-O- β-Glucopyranoside, Stigmasetrol and Sitosterol was confirmed by spectral interpretation. These compounds were tested for their antimicrobial activity and showed inhibition against all the tested bacterial and fungal cultures.


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How to Cite

Ranjitha J, Madonna Shalma, Michael Donatus, Vijayalakshmi S. Isolation of novel phytoconstituents from the stem part of Cleome gynandra Linn and their antimicrobial activity. ijp [Internet]. 2014 Sep. 30 [cited 2024 Feb. 28];6(3):341-5. Available from:



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