A comparative study on total phenolic content, reducing power and free radical scavenging activity of aerial parts of Barleria prionitis

Authors

  • Sunil K. Jaiswal Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, (C.S.I.R), Rana Pratap Marg, P.O. Box 436, Lucknow 226001, Uttar Pradesh, India.
  • Mukesh K. Dubey Department of Pharmaceutical sciences, Diberugarh University, Diberugarh, Assam, India.
  • Sanjeeb Das Department of Pharmaceutical sciences, Diberugarh University, Diberugarh, Assam, India.
  • Arti Raj Verma Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, (C.S.I.R), Rana Pratap Marg, P.O. Box 436, Lucknow 226001, Uttar Pradesh, India.
  • Ch.V. Rao Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, (C.S.I.R), Rana Pratap Marg, P.O. Box 436, Lucknow 226001, Uttar Pradesh, India.

Keywords:

Barleria prionitis, total phenolic content, β carotene bleaching assay, reducing power, free radical scavenging activity

Abstract

Context: Barleria prionitis L. (Family Acanthaceae; commonly known as Vajradanti), is an annual shrub, 1–3 feet high, found throughout tropical Asia and in South Africa. Objective: The aim of the present study was to evaluate the antioxidant activity of 50% ethanolic extract of leaf, flower, and stem of B. prionitis by using β carotene bleaching assay, reducing power and free radical scavenging activity (DPPH and hydroxyl radical scavenging activity). Total phenolic content (TPC) was analysed by the Folin–Ciocalteu colorimetric method using gallic acid as standard and expressed as mg/g gallic acid equivalent (GAE). Results: Total phenolic content (TPC) and antioxidant activity (AOA) in B. prionitis leaves were found to be 67.48 mg/g GAE dry plant material and 79.20%. The B. prionitis leaves exhibited strong free radical scavenging activity as evidenced by the low IC50 values in both DPPH (1,1-diphenyl-2-picryl hydrazyl) (336.15 μg/ml) and hydroxyl radical (568.65 μg/ml) methods. Conclusions: The leaf of B. prionitis possesses high phenolic content, potential antioxidant activity, reducing power & radical scavenging activity in comparison to flower and stem.

References

Ajitha M., Rajnarayana K., Role of

oxygen free radicals in human diseases.

Indian drugs 2001. 38: 545–554.

Ghosal S, Tripathi VK, Chauhan, S.

Active constituents of Emblica officinalis.

Part I. The chemistry and antioxidative

effects of two new hydrolysable tannins,

Emblicanin A and B. Indian Journal of

Chemistry 1996. 35 (B): 941–948.

Badami S, Gupta MK, Suresh B.

Antioxidant activity of the ethanolic

extract of Striga orobanchioides. Journal

of Ethnopharamacology 2003, 85: 227–

Bhattacharya A, Chatterjee A, Ghosal S,

Bhattacharya SK. Antioxidant activity of

active tannoid principles of Emblica

officinalis (amla). Indian Journal of

Experimental Biology 1999. 37: 676–680.

Nadkarni AK. Barleria prionitis.Linn. In

Dr. K. M. Nadkani’s. Indian Material

Medica, 3rd edn, 1994. Reprint Vol.1.

Popular Book Depot; Bombay.

Kiritkar KR, Basu BD. Barleria prionitis

Linn. In Indian Medicinal Plant 3rd edn,

Blatter E, Caicus JF, MhaskarKS

(eds).Sri Satguru Publication:Delhi; 8:

pp.2587-2590.

Singh B, Bani S, Gupta DK, Chandan

BK, Kaul A. Anti-inflammatory activity

of ‘TAF’ an active fraction from the plant

Barleria prionitis Linn. Journal of

Ethnopharmacology 2003. 85(2-3): 187-

Amoo SO, Finnie JF, Staden JV. In vitro

pharmacological evaluation of three

Barleria species. Journal of

Ethnopharmacology 2009. 121(2): 274-

Singh B, Chandan BK, Prabhakar A,

Taneja SC, Singh J, Qazi GN. Chemistry

and hepatoprotective activity of an active

fraction from Barleria prionitis Linn. in

experimental animals. Phytotherapy

Research 2005. 19(5): 391-404.

Gupta RS, Kumar P, Dixit VP, Dobhal

MP. Antifertility studies of the root

extract of the Barleria prionitis Linn.in

male albino rats with special reference to

testicular cell population dynamics. j.

ethanopharmacol 2000. 70: 111-117.

Lu Chen J, Blanc P, Stoddart CA, Mark

B, Rozhan JE, Parkinson N, Ye Z, Cooper

R, Balick M, Nanakorn W, Kernan MR,

New iridoid from the medicinal plant

Barleria prionitis with potent activity

against Respiratory Syncytial Virus. j. Nat

.Prod. 1998. 61: 1295-1297.

Ata A, Kalhari KS, Samarasekera R.

Chemical constituents of Barleria

prionitis and their enzyme inhibitory and

free radical scavenging activities.

Phytochemistry Letters 2009. 2(1): 37-40.

Ragazzi E, Veronese G. Quantitative

analysis of phenolic compounds after

thin-layer chromatographic separation. J

Chromatogr 1973, 77: 369-375.

Rekha PS, Girija K, Ramadasan K,

Antioxidant activity of Brahma rasayana.

Indian Journal of Experimental Biology

39: 447–452.

Emmons CL, Peterson DM, Antioxidant

Activity and Phenolic Contents of Oat

Groats and Hulls. Cereal Chem 1999.

(6): 902-906.

Apati P, Szentmihalyi K, Kristo SZT,

Papp I., Vinkler P, Szoke E, Kery A.

Herbal remedies of Solidago—correlation

of phytochemical characteristics and

antioxidative properties. J Pharma

Biomed. Anal (2003). 32: 1045–1053.

Yen GC, Duh PD. Scavenging Effect of

Methanolic Extracts of Peanut Hulls on

Free-Radical and Active-Oxygen Species.

J Agri Food Chem 1994. 42: 629 –632.

Kroyer GTh, Red clover extract as

antioxidant active and functional food

ingredient. Innovative Food Sci Emer

Tech 2004. 5: 101-105.

Halliwell B, Gutteridge JM, Aruoma OI.

The deoxyribose method: a simple “test

tube” assay for determination of rate

constants for reactions of hydroxyl

radicals. Analytical Biochemistry 1987.

: 215–219.

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Published

30-06-2010

How to Cite

1.
Sunil K. Jaiswal, Mukesh K. Dubey, Sanjeeb Das, Arti Raj Verma, Ch.V. Rao. A comparative study on total phenolic content, reducing power and free radical scavenging activity of aerial parts of Barleria prionitis. ijp [Internet]. 2010 Jun. 30 [cited 2024 Jun. 21];2(2):155-9. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/32

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