Chemical constituent, antibacterial and antioxidant activity of crude extract and oil fraction of L. abyssinica

Authors

  • Fodouop Chegaing Pierre Departement of Biomedical Sciences Lecturer, Fodouop Chegaing Pierre University of Ngaoundéré Cameroon.
  • Donatien Gatsing Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon.
  • Gerald Ngo Teke Department of Medicine, Faculty of Health Sciences, University of Bamenda, P.O. Box 39 Bambili, Cameroon.
  • Xavier Cheseto Internationalcenter of isect Physiology and Ecology (ICIPE), BehaviouralChemistry and Ecology Department. P.O. Box 30772-00100 Nairobi, Kenya.
  • Benjamin Talom Tangue Department of Biomedical Sciences, University of Ngaoundéré, PO. Box 454 Ngaoundéré, Cameroon.
  • Jules-Roger Kuiate Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon.
  • Baldwin Torto Internationalcenter of isect Physiology and Ecology (ICIPE), BehaviouralChemistry and Ecology Department. P.O. Box 30772-00100 Nairobi, Kenya.

Keywords:

Ludwigia abyssinica, GC-MS, Column chromatography, antioxidant, antibacterial

Abstract

The antibacterial and antioxidant activities of CH3Cl/MeOH crude extract and a nonpolar fraction from the CHCl3 phase of Ludwigia abyssinica were investigated using broth dilution method and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay respectively. Chemical composition was determined by gas chromatography coupled with mass spectrometry using Agilent Technologies 7890A GC system-Agilent Technologies 5975C inert XL EI/CI MSD. Bioassays show that L. abyssinica is active on the different test organisms with the MIC ranging from 0.5 to 1.5 mg/ml for the crude extract and 0.2 to 0.5 mg/ml for the non polar oil fraction. The oil extract of L. abyssinica exhibited a good antioxidant activity with radical scavenging activity (IC50) = 89.18 µg/ml which is about 1.5 times the antioxidant activity of ascorbic acid used as control, while that of crude extract was 413.74 µg/ml. From the gas chromatography coupled with mass spectrometry analyses, 12 hydrocarbons were identified with octadecane (5.67%), pentadecane (6.87%) and squalene (57.18%) the major components. These results offer a plat form of using L. abyssinica for alternative and complementary medicine.

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Published

30-06-2014

How to Cite

1.
Fodouop Chegaing Pierre, Donatien Gatsing, Gerald Ngo Teke, Xavier Cheseto, Benjamin Talom Tangue, Jules-Roger Kuiate, Baldwin Torto. Chemical constituent, antibacterial and antioxidant activity of crude extract and oil fraction of L. abyssinica. ijp [Internet]. 2014 Jun. 30 [cited 2024 Nov. 22];6(2):170-6. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/317

Issue

Vol. 6 No. 2 (2014): Apr-Jun

Section

Original Research Articles