Moringa oleifera Lam. leaves prevent Cyclophosphamide-induced micronucleus and DNA damage in mice

Authors

  • Sathya T.N. International Institute of Biotechnology and Toxicology (IIBAT), Kanchipuram district, Padappai, India. 601301.
  • Aadarsh P. International Institute of Biotechnology and Toxicology (IIBAT), Kanchipuram district, Padappai, India. 601301
  • Deepa V. International Institute of Biotechnology and Toxicology (IIBAT), Kanchipuram district, Padappai, India. 601301
  • Balakrishna Murthy P. International Institute of Biotechnology and Toxicology (IIBAT), Kanchipuram district, Padappai, India. 601301

Keywords:

M. oleifera, Anti-genotoxi, Micronucleus assay, Comet assay, Chemoprevention

Abstract

Chemoprotective effect of ethanolic extract of Moringa oleifera Lam leaves was evaluated on cyclophosphamide (CP)-induced genotoxicity in the mouse. Animals were pre-treated with the extract for seven consecutive days at doses of 250, 500, 1000 and 2000 mg/kg b.w. Micronucleus in bone marrow and comet (DNA damage) in the liver were performed. Cyclophosphamide was administered intra-peritoneally on day 7 and Mice were sacrificed after 24 hours. In CP treated animals, statistically significant induction of micronuclei in polychromatic erythrocytes (PCE) was recorded. However, in the animals pre-treated with the extract, the percentage of CP-induced MN decreased with increasing concentration of the extract. Results of comet assay showed similar decrease in DNA damage in mice pre-dosed with the extract. These results point out to the presence of chemopreventive phytoconstituents in the crude extract offering protection against CP-induced genotoxicity in the mouse.

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Published

30-06-2010

How to Cite

1.
Sathya T.N., Aadarsh P., Deepa V., Balakrishna Murthy P. Moringa oleifera Lam. leaves prevent Cyclophosphamide-induced micronucleus and DNA damage in mice. ijp [Internet]. 2010 Jun. 30 [cited 2024 Jun. 21];2(2):147-54. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/31

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