Anti-hyperammonaemic effect of fisetin in rats: A chronotherapeutic study

Authors

  • Murugesan Jayakumar Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
  • Onn Haji Hashim Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  • Perumal Subramanian PROFESSOR, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.

Keywords:

Ammonium chloride, Antioxidants, Circadian, Chronotherapy, Fisetin, Hyperammonaemia

Abstract

Ammonia, a product of degradation of proteins and other nitrogen compounds is toxic at higher concentrations. Fisetin (a naturally occurring flavonoid) is known to exhibit a number of cytoprotective effects. In this study, chronotherapeutic effects of fisetin on ammonium chloride (AC) treated rats is aimed to establish the time point at which the maximum drug effect is achieved. Fisetin (50 mg/kg b.w oral) was administrated to rats at 06:00, 12:00, 18:00 and 00:00 h against AC (100 mg/kg.b.w. i.p) treated Wistar rats (180-200 g). Amelioration of pathophysiology of AC induced hyperammonaemic characteristics by fisetin at different time points were measured by assessing cellular architecture of liver and brain, lipid profile, lipid peroxidation products and antioxidants (superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione) in serum, plasma, liver and brain. The normalization of lipid profile and markers of reactive oxygen species (ROS) homeostasis indicated the protective effect of fisetin. Fisetin administration at 00:00 h showed significant effects on these indices than at other time points (p<0.05; DMRT). This might be due to chronopharmacokinetic and chronopharmacodynamic properties of fisetin along with its action on altered rhythms of lipid profile and markers of ROS homeostasis.

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Published

31-03-2014

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1.
Murugesan Jayakumar, Onn Haji Hashim, Perumal Subramanian. Anti-hyperammonaemic effect of fisetin in rats: A chronotherapeutic study. ijp [Internet]. 2014 Mar. 31 [cited 2024 Dec. 23];6(1):73-81. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/303

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