Modulation of Cellular Calcium Adenosine Triphosphatase in Tissues of Rats Fed with Protein-Deficient Diet Fortified with Moringa oleifera Leaves

Authors

  • Ismaila Olanrewaju Nurain Biochemistry, Assistant Lecturer, Kwara State University Malete, Kwra State, Nigeria
  • Bamisaye F Abraham Biochemistry Unit, Department of Biosciences and Biotechnology, College of Pure and applied sciences, Kwara State University, Malete, P.M.B.1530, Ilorin. Nigeria
  • Ajani O Emmanuel Biochemistry Unit, Department of Biosciences and Biotechnology, College of Pure and applied sciences, Kwara State University, Malete, P.M.B.1530, Ilorin. Nigeria.

Keywords:

Cellular Ca2 -ATPase Activities, Protein deficiency, Moringa oleifera, Protein concentration

Abstract

Calcium Adenosine Triphosphatase is a ubiquitous enzyme and key protein for the maintenance of calcium homeostasis in various cellular compartments. Moringa leave has been reported to be a very good source of dietary protein as well as pool of minerals. So far the effect of varying dietary protein on the activities of Ca2+ pump across cell membranes is yet to be fully explained. In this study, the effects of protein deficiency on Ca2+-ATPase activities were investigated. Twenty four Wistar rats (average weight 119±0.3 g) randomised into four groups of six (6) rats each were used for the study. Group A rats were treated with normal animal diet, group B were placed on protein-deficient diet, Group C were treated with protein deficient diet fortified with fish while Group D were administered with protein deficient diet fortified with Moringa oleifera leaves powder. They were maintained on respective diets for 6 weeks then sacrificed. The skeletal muscle, brain and liver were removed and homogenised. Vanadate is a known cellular Ca2+-ATPase inhibitor and was used to confirm the sensitivity of the enzyme in the homogenate. The protein concentration in each formulated diet was determined to be 9.71%, 2.19%, 23.92% and 31.54% for group A, B, C and D respectively. The enzyme’s activities were highest in group D and lowest in Group B. In the same line, the activities were also highest in the skeletal muscle and lowest in the liver of all the groups. The Km (ATP) and Vmax of the enzyme in all the studied organs were lowest in group D and highest in group B. The results indicate that protein deficiency reduces the activities of Ca2+-ATPase in the entire tissues’ cells and that M. oleifera leave powder is a better activator for the enzyme and therefore a good modulator of Ca2+-ATPase activities

References

. Adewoye, EO, Ige AO, Adeyanju O,

Bekibele, EB. Effect of diet of Varying

Protein concentrations on the Activity

of Erythrocyte Membrane Ca2+Mg2+

ATPase in Dogs. Nig. J. Physiol. Sci.

;25: 67 ă 72.

. Brosnan J. Interorgan amino acid

transport and its regulation. Journal of

Nutrition. 2003;133(6): 2068Să2072S.

. Hoenderop JGJ, Nilius B, Bindels

RJM. Calcium Absorption across

Epithelia. Physiol. Rev. 2005;85: 373-

. Bautista DM, Lewis RS. Modulation of

plasma membrane calcium -ATpase

activity by local calcium microdomains

near CRAC channels in human T

cells. J Physiol. 2004;556 (3):805 -

. Olorunsogo OO. Erythrocyte

membrane Ca2+Mg2+ ATPase in

human protein-energy malnutrition.

Biosc Rep. 1989;9 (93): 359 ă 368.

. Kakengi AMV, Shem MN, Sarwatt SV,

Fujihara T. Can Moringa oleifera be

used as a protein supplement to

ruminants? Asian-Australasian

Journal of Animal Sciences.

;18(1):42-47.

. Maida A, Farooq A, Raziya N, Umer

R, Kazi TG, Nadeem M. Mineral

Composition of Moringa oleifera

Leaves and Pods from Different

Regions of Punjab, Pakistan. Asian

Journal of Plant Sciences, 2005;4:

-421.

. Sutherland JP, Folkard GK, Grant

WD. Natural coagulants for

appropriate water treatment: a novel

approach, Waterlines, 1990;(4):30-32.

. Makkar HPS, Becker K. Nutritional

value and nutritional components of

whole and extracted Moringa oleifera

leaves. Animal Feed Science and

Technology. 1996;63:211 ă 228

. Sarwatt SV, Kapange SS, Kakengi

AMV. The effects on intake,

digestibility and growth of goats when

sunflower seed seed cake is replaced

with Moringa oleifera leaves in

supplements fed with Chloris gayana

hay. Agroforestry systems.

;56:241-247

. Kimoro BN: Potential of selected Multpurpose trees as feed for growing

goats on maize stover basal diet.

MSc. Thesis. Sokoine University of

Agriculture, Morogoro ă Tanzania.

. National Research Council, Guide for

the Care and Use of Laboratory

Animals. National Academy Press,

Washington, DC. 1999.

. Ghazala R, Tabinda AB, Yasa A.

Growth Response Of Juvenile Grass

Carp (Ctenopharyngodon Idella) Fed

Isocaloric Diets With Variable Protein

Levels. The Journal of Animal & Plant

Sciences, 2011;21(4): 850-856

. Olugbemi TS, Mutayoba SK, Lekule

FP. Effect of Moringa (Moringa

oleifera) Inclusion in Cassava Based

Diets Fed to Broiler Chickens.

International Journal of Poultry

Science. 2010;9 (4): 363-367.

. Gornall AG, Bardawill CJ, David MM.

Determination of serum protein by

means of Biuret reaction. J. Biol.

Chem. 1949;177: 571-576.

. Bewaji CO, Olorunsogo OO.

Bababunmi EA. Comparison of the

membrane bound (Ca2+-Mg2+)-

ATPase in erythrocytes ghost

membrane from some mammalian

species. Comp. biochem. Physiol.

;828: 117-122.

. Fiske CH, Subbarow. The colorimetric

determination of phosphorus. J. Biol.

Chem. 1925;66: 375 ă 400.

. Racay P, Kaplan P, Lehotsky J.

Control of Ca2+ homeostasis in

neuronal cells. Gen. Physiol. Biophys.

;15:193ă210.

. Egelman DM, Montague, PR. Calcium

dynamics in the extracellular space of

mammalian neural tissue. Biophys. J.

;76:1856ă1867.

. Guerini D. The Ca2+-pumps and the

Na+/Ca2+ exchangers. Bio. Metals.

;11: 319ă321.

. Muchal S, Cuschieri A, Al-Bader AA.

Intracellular distribution of Ca2+-Mg2+

adenosine triphosphatase (ATPase) in

various tissues. J Anat. 1989;162:

-24

. Ludmia Y, Mirosaw S. Plasma

membrane Ca2+-ATPase in excitable

and non excitable cells. Acta

Biochimica Polonika. 2000; 47 (3):

-539

. Johnson NE, Alcantara EN, Linkswiler

H. Effect of level of protein intake on

urinary and fecal calcium and calcium

retention of young adult males. J.

Nutr. 1970;100: 1425-1430

. Younghee K, Hellen ML. Effect of

Level of Protein Intake on Calcium

Metabolism and on Parathyroid and

Renal Function in the Adult Human

Male. J. Nutr. 1979;109:1399-1404

. Kerstetter JE, OÊBrien KO, Insogna

KL. Dietary protein, calcium

metabolism, and skeletal homeostasis

revisited. Am J Clin Nutr. 2003;

:584Să592S

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Published

31-12-2013

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Ismaila Olanrewaju Nurain, Bamisaye F Abraham, Ajani O Emmanuel. Modulation of Cellular Calcium Adenosine Triphosphatase in Tissues of Rats Fed with Protein-Deficient Diet Fortified with Moringa oleifera Leaves. ijp [Internet]. 2013 Dec. 31 [cited 2024 Dec. 6];5(4):519-28. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/294

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