High performance thin layer chromatography fingerprint profile of ingredients and formulations of Shatavaryadi churna: an ayurvedic formulation

Authors

  • Puspendra Kumar Department of Pharmaceutical Sciences Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India Zip Code-835215 India.
  • Shivesh Jha Department of Pharmaceutical Sciences Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India Zip Code-835215 India.
  • Tanveer Naved Amity Institute of Pharmacy, Amity University, Noida-201301, Uttar Pradesh, India. India

Keywords:

Shatavaryadi churna, High Performance Thin Layer Chromatography (HPTLC), Fingerprinting, Asparagus racemosus, Ayurvedic formulation

Abstract

Objective: To develop the finger print of medicinally important ayurvedic formulation Shatavaryadi churna and its ingredients for presence of phytoconstituents in different extracts. Methods: Petroleum ether, chloroform, ethyl acetate and methanolic extract of all the ingredients and formulations were developed in the mobile phase n-hexane: diethyl ether (3:1), n-hexane: ethyl acetate: formic acid (6:4:0.2) n-hexane: ethyl acetate: formic acid (6:4:0.2) and toluene: ethyl acetate: formic acid (5:4:0.2) respectively using standard procedures and scanned under UV at suitable wavelength. Results: The HPTLC fingerprinting of all the extracts has shown several peaks with different Rf values. Conclusion: This fingerprint profile would be helpful in the identification and authentication of the selected ingredients and the formulations.

References

Dalal S, Kataria SK, Sastry KV, Rana SVS. Phytochemical screening of methanolic extract and antibacterial activity of active principles of Hepatoprotective herb Eclipta alba. Ethnobot Leaf. 2010; 14: 248-258.

Kamboj A, Saluja AK. HPTLC finger print profile of extracts from dried aerial parts of Ageratum conyzoides L. in different solvents. Asian J Pharm Sci. 2011; 6(2): 82-88.

Hariprasad P, Ramakrishnan N. Chromatographic finger print analysis of Rumex vesicarius L. by HPTLC Technique. Asia Pac J Trop Biomed. 2012; S57-S63.

World Health Organization. WHO monographs on medicinal plants, Vol-1, Geneva; 1998. p. 1-15.

Pathak RR. Ayurved Sarsangrah. 12th ed. Allahbad, India: Shree Baidyanath Ayurved Bhawan Ltd.; 2003. p. 603-604.

Gong F, Wang BT, Chau FT, Liang YZ. HPLC Data preprocessing for chromatographic fingerprint of herbal medicine with chemometric approaches. Analyt let. 2005; 38: 2475-2492.

Liang YZ, Xie P, Chan K. Quality control of herbal medicines. J Chromato B. 2004; 812: 53-70.

Peishan X, Sibao C, Yi-zeng L, Xianghong W, Runtao T, Roy U. Chromatographic fingerprint analysis-a rational approach for quality assessment of traditional Chinese herbal medicine. J Chromato A. 2006; 1112(1-2): 171-180.

Maffat CA. Clarke’s analysis of drugs and poisons. London: Pharmaceutical Press, 2001. p. 392.

Downloads

Published

31-12-2013

How to Cite

1.
Puspendra Kumar, Shivesh Jha, Tanveer Naved. High performance thin layer chromatography fingerprint profile of ingredients and formulations of Shatavaryadi churna: an ayurvedic formulation. ijp [Internet]. 2013 Dec. 31 [cited 2024 Dec. 6];5(4):467-74. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/286

Issue

Section

Original Research Articles