Antidiabetic activity of Cassia sophera in STZ induced diabetic rats and its effect on insulin secretion from isolated pancreatic islets

Authors

  • Poonam Sharma Department of Zoology, Institute of Basic Sciences Assistant Professor, Bundelkhand University,Jhansi,UP,INDIA India.
  • Rambir Singh Department Of Biomedical Sciences Associate Professor, Bundelkhand University, Jhansi,UP-INDIA India.
  • Priyanka Bhardwaj Department Of Biomedical Sciences Project Fellow, Department Of Biomedical Sciences Project Fellow.

Keywords:

Cassia sophera, Antidiabetic activity, Pancreatic islets, Nicorandil

Abstract

The aim of the study was to evaluate the antidiabetic effect of Cassia sophera seed extracts in streptozotocin (STZ) induced diabetic Wistar rats. Aqueous extract of Cassia sophera (AECS) showed maximum fall (60.88%, p<0.01) in 0 to 1 h fasting blood glucose during glucose tolerance test in normal healthy rats. Since AECS exhibited potential hypoglycemic activity, it was further studied for antidiabetic effect in STZ induced diabetic rats. The oral effective dose (ED50) of AECS was 200 mg/kg body weight which produced a fall of (70.50%, p<0.01) in diabetic rats. Treatment with AECS significantly reduced the elevated glycosylated haemoglobin (45.67%, p<0.05) and increased insulin level (40.6%, p<0.01). Total cholesterol (19.05%) low density lipoprotein (21.41%), triglyceride (57.87%) levels and very high density lipoprotein (57.85%) were significantly decreased (p<0.05) whereas, high density lipoprotein was significantly increased (38.58%, p>0.05) in diabetic rats. Since AECS treatment resulted in significant increase in serum insulin, its effect on insulin secretion in isolated pancreatic islets was also studied. AECS stimulated insulin secretion from the isolated islets at 3.3 and 16.7mM glucose. The effect of AECS was dose dependent. AECS induced enhancement in insulin secretion was not diminished by nicorandil, suggesting that AECS induced insulin secretion was independent of K-ATP channels of β cells.

References

Nickavar B and Yousefian N. Evaluation of α-amylase inhibitory activities of selected antidiabetic medicinal plants. Journal fur Verbraucherschutz und Lebensmittelsicherheit. 2011;6(2):191-195.

Xie W, Zhao Y, Gu D, Du L, Cai G, Zhang Y. Scorpion in combination with gypsum: novel antidiabetic activities in streptozotocin-induced diabetic mice by up-regulating pancreatic PPARg and PDX-1 expressions. Evidence-Based Complementary and Alternative Medicine Article ID 683561, 2011;p.9.

Diagnosis and classification of diabetes mellitus. Diabetes care 2008;31:55-60.

Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol. 2002;81:81-100.

Qureshi SA, Nawaz A, Udani SK, Azmi B. Hypoglycaemic and hypolipidemic activities of Rauwolfia serpentina in STZ-induced diabetic rats. Int J Pharmacol. 2009;5:323-326.

Iweala EEJ and Oludare FD. Hypoglycemic effect, biochemical and histological changes of Spondias mombin Linn. And Parinari polyandra benth. Seeds ethanolic extracts in STZ-induced diabetic rats. J Pharmacol Toxicol. 2011;6:101-112.

Ekor M, Odewabi AO, Bakre AG, Oritogun KS, Ajayi TE, Sanwo OV. Comparative evaluation of the protective effect of the ethanolic and methanolic leaf extracts of Sida acuta against hyperglycemia and alternations of biochemical and haematological indices in STZ diabetic rats. J Pharmacol Toxicol. 2010;5:1-12.

Nadkarni AK.. Indian Materia Medica. 3rd ed. Popular Pvt. Ltd., Mumbai, India.1982;p.634- 635.

Shastri AD. In: Bhaisajyaratnawali, Chaukhamba Sanskrit Sanmsthan, Varanasi, 1981;p. 619, 623-625.

Kirtikar KR and Basu BD. In: Indian medicinal plants, Dehradun India. International book distributors, II edition 1935, Vol. II reprint, 1999;p.864.

Jalalpure SS, Patil MB, Aruna P, Shah BN, Salahuddin MD. Antidiabetic activity of Cassia auriculata seeds in STZ induced diabetic rats. Niger J Nat Prod Med. 2004;8:22-3.

Nirmala A, Eliza J, Rajalakshmi M, Priya E, Daisy P. Effect of Hexane Extract of Cassia fistula barks on Blood Glucose and Lipid Profile in Streptozotocin Diabetic Rats. International J of Pharmcology. 2008;4(4):292-296.

Singh R, Seherawat A, Sharma P. Hypoglycemic antidiabetic and toxicological evaluation of Momordica dioica fruit extracts in alloxan induced diabetic rats. Journal of Pharmacology and Toxicology. 2011;6:454-467.

Friedewald WT, Levy RI, Friederickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without the use of the preparative ultracentrifuge. Clin Chem. 1972;18:499-502.

Lacy PE and Kostianovsky M. Method for the isolation of intact islets of langerhans from the rat pancreas. Diabetes. 1967;16:35-9.

Pipeleers DG and Pipeleers-Marichal MA. A method for the purification of single A B and D cells and for the isolation of coupled cells from isolated rat islets. Diabetologia. 1981;20:654-663.

Sharma P, Singh M, Sudershan KA, Singh R. The Evaluation of in vitro toxicity of the protein (LL4218) isolated from leaves of Argemone mexicana in hepatoma derived cell lines (Hep G 2) using multiplexing approach. Journal of Natural Pharmaceuticals. 2011;2:179-183.

Akhtar FM and Ali MR. Study of the anti diabetic effect of a compound medicinal plant prescription in normal and diabetic rabbit. J Pak Med Assoc. 1980;34:239-44.

Suba V, Murugesan T, Arunachalam G, Mandal SC, Sahu BP. Anti diabetic potential of Barleria lupilina extract in rats. Phytomed. 2004;11:202-5.

Rahman Q and Zaman K. Medicinal plants with hypoglycemic activity. J Ethnopharmacol. 1989;26:1-55.

Halim EM and Hussain A. Hypoglycemic, hyplipidemic and antioxidant properties of combination of Curcumin from Curcuma longa Linn. and partially purified product from Abroma augusta Linn. in streptozotocin induced diabetes. Indian Clin Biol. 2002;17:33-43.

Latha M and Pari L. Effect of an aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and some polyol pathway enzymes in experimental rat diabetes. Brazilian J Med Biol Res. 2004;37:577-86.

Ozturk Y, Altan VM, Yildizoglu A. Effect of experimental diabetes and insulin on smooth muscle functions. Pharmacol Rev. 1996;48:69-112.

Ayol RG, Amupitan JO, Yimin Z. Cytotoxicity and antimicrobial studies of 1, 6, 8- trihydroxy-3-methyl-anthraquinone (emodin) isolated from the leaves of Cassia nigricans. Afr J biotech. 2007;6:1276-9.

Babu V, Gangadevi T, Subramoniam A. Antidiabetic Activity of Ethanol Extract Of Cassia kleinii Leaf in Streptozotocin-Induced Diabetic rats and Isolation of an Active Fraction and Toxicity Evaluation of the Extract. Indian Journal of Pharmacology. 2003;35:290-296.

Nam J and Hyunju C. Effect of butanol fraction from Cassia tora L. seed on glycemic control and insulin secretion in diabetic rats. Nutritional research and practice. 2008;2(4):240-246.

Anand P, Murali KY, Tandon V, Chandra R, Murthy PS. Preliminary studies on antihyperglycemic effect of aqueous extract of Brassica nigra (L.) Koch in streptozoticin induced diabetic rats. Indian J of Experimental Biology. 2007;45:696-701.

Achyut NK , Gupta RK, Watal G . Hypoglycemic effects of Murraya koenigii on normal and alloxan-diabetic rabbits. Journal of Ethanopharmacology. 2005;95(2):247-251.

Chatterjee MN and Shinde R. Textbook of Medical Biochemistry. Metabolism of Carbohydrates, JayPee Medical Publishers, Delhi 2000;p. 421.

Annamalai P, Subramaniam S, Kodukkur VP. Effect of Casearia esculenta root extract on blood glucose and plasma antioxidant status in Streptozotocin diabetic rats. Pol J Pharmacol. 2003;55:43-49.

Jayasri MA, Gunasekaran S, Radha A, Mathew TL. Anti-diabetic effect of Costus pictus leaves in normal and streptozotocin-induced diabetic rats. Int J Diabetes & Metabolism. 2008;16:117-122.

Padmini K and Chakrabarti CH. Effects of Bittergourd (Momordica charantia) seed and glibenclamide in streptozotocin-induced diabetes mellitus. Indian J Exp Biol. 1982;20:232-235.

Arulselvan P and Subramanian SP. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra structural changes of pancreatic β-cells in experimental diabetes in rats. Chemico-Biological Interactions. 2007;165:155-164.

Arvind K., Pradeep R, Deepa R , Mohan V. Diabetes and coronary artery diseases. Indian J. Med. Res. 2002;116:163-176.

Palumbo PJ. Metformin: Effect on cardiovascular risk factor in patients with noninsulin dependent diabetes mellitus. J. Diabetes Complications. 1998;12: 110-119.

Lanjhiyana S, Garabadu D, Ahirwar D, Bigoniya P, Rana AC, Chandra PK, Lanjhiyana KS, Karuppaih M. Antidiabetic activity of methanolic extract of stem bark of Elaeodendron glaucum Pers. in STZized rat model. Pelagia Research Library, Advances in Applied Science Research 2011;2 (1):47-62.

Murali B, Upadhyaya UM, Goyal RK.. Effect of chronic treatment with Enicostemma littorale in non-insulin dependent diabetic (NIDDM) rats. J Ethnopharmacol 2002;81:199-204.

Koenig RJ, Perterson CM, Jones RL, Saudek C. Correlation of glucose regulation and HbA1c in diabetes mellitus. New Engle J Med. 1976;295:417.

Al-Yassin D and Ibrahim K. A minor haemoglobin fraction and the level of fasting blood glucose. J Fac Med Univ Baghdad. 1981;23:373-380.

Maritim AC, Sanders RA, Watkins JB. 3rd Diabetes, oxidative stress, and antioxidants: A review. J Biochem Mol Toxicol. 2003;17:24-38.

Patlak M. New weapons to combat an ancient disease: treating diabetes. FASEB J. 2002;16(14):1853.

Bhonde RR, Parab PB, Sheorin VS. An in vitro model for screening oral hypoglycemics. In vitro Cellular Developmental Biology-Animal. 1999;35:366-8.

Persaud SJ, Majed H, Al Raman A, Jones PM. Gymnema sylvestre stimulate insulin release in vitro by increased membrane permeability. Journal of Endocrinology. 1999;62: 207-212.

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Published

30-09-2013

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Poonam Sharma, Rambir Singh, Priyanka Bhardwaj. Antidiabetic activity of Cassia sophera in STZ induced diabetic rats and its effect on insulin secretion from isolated pancreatic islets. ijp [Internet]. 2013 Sep. 30 [cited 2024 Oct. 8];5(3):314-21. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/267

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