Screening of antioxidant, anti-acetylcholinesterase and antifungal activities and HPLC-MS identification of the bioactive phenolics of Eclipta alba (L.) Hassk.
Keywords:
E. alba, Antioxidant, anti-acetylcholinesterase, Anti-fungal, Polyphenols HPLC-MSAbstract
Eclipta alba is commonly used in traditional medicine for several ethnobotanical properties. Researchers have isolated several bioactive compounds of this plant. In Burkina Faso, this medicinal plant is mainly used for its antifungal properties. Our study aimed to evaluate the antioxidant, acetylcholinesterase inhibitory, antifungal activities and to quantify polyphenols from Eclipta alba extracts using HPLC-MS. The crude extract (CE) and fractions of this plant were used for these activities through standard methods. Ethyl acetate fraction (EAF) and acetonitrile fraction (ACNF) have been the potential inhibitors of the liver lipids peroxidation (According to the thiobarbituric acid method) with the percentages of 65.48% and 65.13%, respectively. Concerning the ABTS radical cation scavenging capacity descript by Guenné, ACNF (67.28 mg T/g of extract) and butanol fraction (BF) (66.03 mg T/g of extract) have presented the highest activities. BF has also presented an interesting acetylcholinesterase (AChE) inhibitory activity with a percentage of 55.82%. For antifungal activity, the crude extract and ACNF have given the better MIC values against C. albican and T. beigelii with a concentration of 1.25 mg/mL using microplate method descript by Eloff. The strain C. krusei was only sensitive to butanol fraction. The polyphenols identified and quantified using HPLC-MS are: gentisic acid, caffeic acid, chlorogenic acid, p-coumaric acid, apigenin, luteolin and quercetin. The butanol fraction of E. alba presented the best activities for the tests performed; this fraction is a good ways to isolate new anti-fungal and anti-acetylcholinesterase compounds.
References
. Nacoulma OG: Plantes médicinales et
pratiques médicales traditionnelles au
Burkina Faso: Cas du plateau central,
Tome II. Thèse de doctorat dÊétat ès
sciences naturelles, Université de
Ouagadougou; Département de
Biochimie-Microbiologie, 1996.
. Gautam R, Saklani A, Jachak SM.
Indian medicinal plants as a source of
antimycobacterial agents. Journal of
Ethnopharm 2007; 110: 200-234.
. Panchal AH, Patel RK,
Pundarikakshudu K, Bhandari A.
Preliminary Phytochemical Screening
and Evaluation of Anti-inflammatory
Activities of Extract of Leaves of
Eclipta alba L. (Asteraceae). Journal
of Pharmacognosy and Herbal
Formulations 2011; 1 (6): 28-36.
. Guenné S, Ouattara N, Hilou A, Millogo
JF, Nacoulma OG. Antioxidant,
enzyme inhibition activities and
polyphenol contents of three
Asteraceae species used in burkina
faso traditionally medicine. Int J
Pharm Pharm Sci 2011; 3 (5): 524-
. Syed DS, Deepak M, Yogisha S,
Chandrashekar AP, Muddarachappa
KA, DÊSouza P, Agarwal A,
Venkataraman BV. Trypsin Inhibitory
Effect of Wedelolactone and
Demethylwedelolactone. Phytother.
Res. 2003; 17: 420-421.
. Pithayanukul P, Laovachirasuwan S,
Bavovada R, Pakmanee N, Suttisri R.
Anti-venon potential of butanolic
extract of Eclipta prostrate against
Malayan pit viper venon. Journal of
Ethnopharm 2004; 90: 347-352.
. Tewtrakul S, Subhadhirasakul S,
Cheenpracha S, Karalai C. HIV-1
Protease and HIV-1 Integrase
Inhibitory Substances from Eclipta
prostrata. Phytother. Res. 2007; 21:
-1095.
. Reddy SL, Fayazuddin Md, Reddy
NCG, Ahmad A, Reddy GS, Raod PS,
Reddy BJ, Fros RL. Characterisation
of bhringaraj and guduchi herb by ICP
MS analysis, optical absorption,
infrared and EPR spectroscopic
methods. Spectrochimica Acta Part A
; 71: 31-38.
. Lee MK, Ha NR, Yang H, Sung SH,
Kim GH, Kim YC. Antiproliferative
activity of triterpenoids from Eclipta
prostrata on hepatic stellate cells.
Phytomedicine 2008; 15: 775-780.
. Lee MK., Ha N.R., Yang H., Sung
S.H., Kim YC. Stimulatory
Constituents of Eclipta prostrata on
Mouse Osteoblast Differentiation.
Phytother. Res. 2009; 23: 129- 131.
. Ebrahimi A, Schluesener H. Natural
polyphenols against
neurodegenerative disorders:
Potentials and pitfalls. Ageing
Research Reviews 2012; 11: 329-
. Su XYZ, Wang, Liu J. In vitro and in
vivo antioxidant activity of Pinus
koraiensis seed extract containing
phenolic compounds. Food
Chem.2009; 117 (4): 681- 686.
. Sombié PEAD, Hilou A, Coulibaly AY,
Tibiri A, Kiendrebeogo M, Nacoulma
OG. Brain protective and Erythrocytes
Hemolysis Inhibition Potencials from
Galls of Guiera senegalensis Gmel
(Combretaceae). Journal of
Pharmacology and Toxicology 2011; 6
(4): 361-370.
. Kiendrebeogo M, Coulibaly AY, Nebie
RCH, Zeba B, Lamien CE, LamienMeda A, Nacoulma OG.
Antiacetylcholinesterase and
antioxidant activity of essential oils
from six medicinal plants from Burkina
Faso. Rev. Bras. Farmacogn. Braz. J.
Pharmacogn 2011; 21 (1): 63-69.
. Bauer AW, Kirby WMM, Sherris JC,
Turck M. Antibiotic susceptibility
testing by a standardized single disk
method. Am J Clin Pathol. 1966; 43:
-496.
. Paulsamy S, Thambiraj J. Evaluation
of antimicrobial efficacy of the folklore
medicinal plant, Acacia caesia (L.)
Wild. Asian J Pharm Clin Res 2011; 4
(3): 103-105.
. Eloff JN. A sensitive and quick
microplate method to determine the
minimal inhibitory concentration of
plant extracts for bacteria. Planta
Medica 1998; 64: 711-713.
. Salem HF, Eid KAM, Sharaf MA.
Formulation and evaluation of silver
nanoparticles as antibacterial and
antifungal agents with a minimal
cytotoxic effect. International Journal
of Drug Delivery 2011; 3 (2): 293-304.
. Cai Y, Luo Q, Sun M, Corke H.
Antioxidant activity and phenolic
compounds of 112 traditional Chinese
medicinal plants associated with
anticancer. Life Sci. 2004; 74 (17):
-2184.
. Xu Y, Zhang J, Xiong L, Zhang L, Sun
D, Liu H. Green tea polyphenols
inhibit cognitive impairment induced
by chronic cerebral hypoperfusion via
modulating oxidative stress. Journal of
Nutritional Biochemistry 2010; 21:
-748.
. Weng CJ, Chen MJ, Yeh CT, Yen GC.
Hepatoprotection of quercetin against
oxidative stress by induction of
metallothionein expression through
activating MAPK and PI3K pathways
and enhancing Nrf2 DNA-binding
activity. New Biotechnology 2011; 28
(6): 767- 777.
. Singh K, Singh N, Chandy A,
Manigauha A. In vivo antioxidant and
hepatoprotective activity of methanolic
extracts of Daucus carota seeds in
experimental animals. Asian Pacific
Journal of Tropical Biomedicine 2012:
-388.
. Tabassum N, Agrawal SS.
Hepatoprotective activity of Eclipta
alba hassk. against paracetamol
induced hepatocellular damage in
mice. JK - Practitioner 2004; 11 (4):
-280.
. Kim DK, Lee SH., Choi JH, Lillehoj
HS, Yu MH, Lee GS. The butanol
fraction of Eclipta prostrata (Linn)
effectively reduces serum lipid levels
and improves antioxidant activities in
CD rats. Nutr. Res 2008; 28: 550-554.
. Kim DK, Lee SH, Hong JH, Lillehoj
HS, Park HJ, Rhie SG, Lee GS. The
butanol fraction of Eclipta prostrata
(Linn) increases the formation of brain
acetylcholine and decreases oxidative
stress in the brain and serum of
cesarean-derived rats. Nutr. Res.
; 30 (8): 579ă584.
. Abdel-Kader MS, Bahler BD, Malone
S, Werkhoven MSM, Troon F, David,
Wisse JH, Bursuker I, Neddermann
KM, Mamber SW, Kingston DGI.
DNA-Damaging Steroidal Alkaloids
from Eclipta alba from the Suriname
Rainforest. J. Nat. Prod. 1998; 61
(10): 1202- 1208.
. Zhang L, Cao H, Wen J, Xu M. Green
tea polyphenol (î)-epigallocatechin- 3-
gallate enhances the inhibitory effect
of huperzine A on
acetylcholinesterase by increasing the
affinity with serum albumin. Nutr.
Neurosci 2009; 12: 142-148.
. Guenné S, Hilou A, Ouattara N,
Nacoulma OG. Antibacterial activity
and Phytochemical composition of
extracts of three medicinal Asteraceae
species from Burkina Faso. Asian J
Pharm Clin Res 2012; 5 (Suppl 2): 37-
. Ryu HW, Curtis-Long MJ, Jung S,
Jeong IY, Kim DS, Kang KY, Park KH.
Anticholinesterase potential of
flavonols from paper mulberry
(Broussonetia papyrifera) and their
kinetic studies. Food Chemistry 2012;
: 1244-1250.
