Phytochemical Investigation and Antibacterial activity of leaf and stem extracts of Sapium indicum Linn
Keywords:
Mangrove, Sapium indicum, phytochemical screening, antimicrobial activityAbstract
Plants have provided a source of inspiration for novel drugs compounds. In this study, the antibacterial activity was of Sapim indicum Linn. collected from mangroves area of Odisha was made against some bacterial pathogens by disc diffusion method. All the four crude extracts (methanol, chloroform, hexane, and petroleum ether) tested, showed significant antimicrobial activity with zone of inhibitions ranging between 9-31 mm. Among all the solvent, methanol extracts of both stem and leaf exhibited the highest inhibition zone against Salmonella typhi. Further evaluation of minimal inhibitory concentration (MIC) showed values ranging from 4-5.6 mg/ml. Preliminary phytochemical screening revealed the presence of alkaloids, steroids, triterpenoids, saponins, flavonoids, reducing sugars and protein in almost all extract. The present study of in vitro antibacterial assay and preliminary phytochemical analysis may open new vistas for identifying potential bioactive molecules for chemical, pharmacological and herbal industry.
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