n vitro antioxidant activity and polyphenol estimation of methanolic extract of endangered medicinal tree species, Hildegardia populifolia (Roxb.) Schott & Endl.

Authors

  • Saradha .M Department of Botany, Kongunadu Arts and Science College, Coimbatore- 641 029.
  • S. Paulsamy Department of Botany, Kongunadu Arts and Science College, Coimbatore- 641 029.

Keywords:

Hildegardia populifolia, free radical scavenging activity (DPPH), hydroxyl radical scavenging activity, reducing power activity, ABTS· , metal chelating activity

Abstract

Antioxidant property of methanolic leaf and stem bark extracts of the endangered medicinal tree species, Hildegardia populifolia was evaluated by studying the contents of total phenolics, tannins and flavonoids, free radical scavenging activity using 1,1-diphenyl-2-picryl hydrozyl (DPPH), hydroxyl radical scavenging activity, reducing power activity, ABTS·+ assay and metal chelating activity. The results of the study revealed that both the parts studied were found to have potent antioxidant activity against DPPH, hydroxyl and ABTS·+ radicals with the IC50 value of 92.86 and 309.83 for methanolic extracts of leaf and stem bark respectively for DPPH radicals and 820.30 (leaf) and 456.71 (stem bark) for hydroxyl radicals. Therefore methanolic extracts of both leaf and stem bark of H. populifolia can be considered as a new potential source of natural antioxidants for pharmaceutical industries.

References

. Ahmed M, Akthar MS, Malik T, Gilani

AH. Hypoglycaemic action of the

flavonoids fraction of Cuminum

nigrum seeds. Phytother. Res. 2000;

:103-106

. Atawodi SE. Antioxidant potential of

African medicinal plants, Afr J

Biotechnol. 2005; 4(2): 128-133.

. Badami S, Moorkoth S,

Rammanoharsingh RS, Kannan E,

Bhojraj S. Antioxidant activity of

Coesalpinia sappan heart wood. Biol

Pharm Bull. 2003; 26(11): 1534-

. Basniwal PK, Suthar M, Rathore GS,

Gupta R, Kumar V, Pareek A, Jain

D. In vitro antioxidant activity of hot

aqueous extract of Helicteres isora

linn. Fruits. Natiral Product

Radiance. 2009; 8(5): 483-487.

. Blois MS. Antioxidants determination

by the use of a stable free radical.

Nature. 1958; 26: 1199-1200.

. Borokini TI, Omotayo FO.

Phytochemical and ethanobotanical

study of some selected medicinal

plants from Nigeria. Journal of

Medicinal Plants Research. 2012;

(7): pp. 1106-1118.

. Chew YL, Goh JK, Lim YY.

Assessment of in vitro antioxidant

capacity and polyphenolic

composition of selected medicinal

herbs from leguminosae family in

Peninsular Malasia. Food Chem.

; 116: 13-18.

. Dinis TCP, Maderia VMC, Almeida

LM. Action of phenolic derivatives

(acetoaminophen, salycillate, and 5

aminosalycillate) as inhibitors of

membrane lipid peroxidation and as

peroxyl radical scavengers. Arch

Biochem Biophys. 1994; 315:161-

. Dreosti IE. Antioxidant phenols in tea,

cocoa and wine. Nutrition.2000;

(16): 692-694.

. Duh PD, Tu YY, Yen GC.

Antioxidant activity of water extract

of Harng Jyur Chrysanthemum

morifolicum Romat Lebensm- Wiss.

Ttechnol. 1999; 32:269-277.

. Gafner F, Msonthi JD, Hostettmann

K. Mlluscicidal saponins from

Talinum tenuissimum Dinter Helvet.

Chim Acta. 1985; 68:555-558.

. Halliwell B. Antioxidants the basicswhat they are and how to evaluate

them. Adv pharmcol. 1997; 38:3-20.

. Hassig A, Liang WX, Shwabl K,

Stampfl K. Flavonoids and tannins

plant based antioxidants with

vitamin character. Med Hypotheses.

; 52: 471-481.

. Hatano T, Edamatsu R, Mori A.

Effect of interaction of tannins and

related polyphenols on superoxide

anion radical and on DPPH radical.

Chem Pharm Bull. 1989; 37:2016-

. Katalinic V, Milos M, Kulisic T, Jukic

M. Screening of 70 medicinal plant

extracts for antioxidant capacity and

total phenols. Food chem.. 2006; 94:

-557.

. Keowmaneechai E, McClements DJ.

Influence of EDTA and citrate on

thermal stability of whey protein

stabilized oil-in-water emulsions

containing calcium chloride. Food

Res Int. 2006; 39:230-239.

. Klipstein – grobusch K, Launer LJ,

Geleijnse JM, Boeing H, Hofman A,

Witterman JC. Serum carotenoids

and antherosclerosis. 2000;

(1):49-56.

. Koleckar V, Kubikova K, Rehakova

Z, Kuca K, Jun D, Jahodar L,Opletal

L. Condensed and hydrolysable

tannins as antioxidants influencing

the health. Mini. Rev. Med. Chem.

; 8: 436-447.

. Moeller SM, Jacques PF, Blumberg

JB. The potential role of dietary

xanthophylls in cataract and agerelated macular degeneration.

Journal of American College

Nutrition. 2000; 19(5): 5225-5278.

. Morris MC, Bekett LA, Scherr PA,

Hebert LE, Bennett DA, Field TS,

Evans DA. Vitamin E and Vitamin C

supplement use and risk of incident

Alzheimer disease. Alzheimer

Disease and Associated Disorders.

; 12(3): 121-126.

. Ogawa K, Suzuki K, Okutsu M,

Yamazaki K, Shinkai S. The

association of elevated reactive

oxygen species leves from

neutrophils with lowgrade

inflammation in the elderly. Immunity

and ageing. 2008; 5(13): 1-8.

. Pattanaik P, Parida S. Antioxidant

and Anti-Inflammatory screening of

barks of Pterospermum acerifolium

(Sterculiaceae). International

Journal of Pharma World

Reasearch. 2010; 1(3): 1-19.

. Pratt DE. Natural antioxidant from

plant material. In: IMT, Huang, CT.

HO & CY Lee (Eds). Phenolic

compounds in food and their effects

on health. New York American

Chemical Society. 1992; pp 54-72.

. Pullaiah T. Medicinal plants in India.

Regency Publications, New Delhi.

. Re R, Pellegrini N, Proteggente A,

Pannala A, Yang M, Rice Evans C.

Antioxidant activity applying an

improved ABTS radical cation

decolorization assay. Free Radical

Bio Med. 1999; 26:1231-1237.

. Salah N, Miller NJ, Paganga G,

Tijburg L, Bolvoell GP Rice Evans

C. Polyphenolic flavonols as

scavengers of aqueous phase

radicals and as chain-breaking

antioxidants. Arch biochem

Biophys.1995; 322(2):339-346.

. Shah R, Guclu K, Demirata K, Celik

SE, Isil Berker K. Comparative

evaluation of various total

antioxidant capacity assays. Applied

to phenolic compounds with te

CUPRAC Assay. Molecules. 2007;

:1496-1547.

. Shahidi F, Wanasundara PKJPD.

Phenolic antioxidants. Food Sci

Nutr. 1992; 32:67-103.

. Shyur, Lie-Fen, Tsung Jieh-hen,

Chen Ie-Hsin, Chiu Chih- Yang, Lo

Chiu-Ping. Antioxidant properties of

extracts from medicinal plants

popularly used in Taiwan, Int J Appl

Sci Eng. 2005; 3(3):195-202.

. Siddhuraju P, Becker K. Antioxidant

properties of various solvent

extracts of total phenolic

constituents from three different

agroclimatic origins of Drumstick

tree (Moringa oleifera Lam.) leaves.

Journal of Agricultural and Food

Chemistry. 2003; 51(8): 2144 –

. Slattery ML, Benson J, Curtin K, Ma

KN, Schacffer D, Pootter JD.

Carotenoids and colon cancer.

American Journal of clinical

Nutrition. 2000; 7(2): 575-582.

. Tanaka M, Kuei CW, Nagashina Y.

Application of antioxidative maillrad

reaction produces from histidine and

glucose to sardine products. Nippon

Suisan Gakkaishi. 1998; 47: 1409-

. Van Acker SABE, Van den vijgh

WJF, Bast F. Structural aspects of

antioxidant activity of flavonoids.

Free Rad Bio Med. 1996; 20(3):

-342.

. Varaprasad B, Katikala PK, Naidu

KC, Penumajji S. Antifungal activity

of selected plant extracts against

pytopathogenic fungi Aspergillus

niger. Indian Jounal of Science and

Technolog. 2009; 2(4): 87-90.

. Yang, guo J, yuan J. In vitro

antioxidant properties of rutin

Lebensm. Wiss Technol. 2008; 41:

-1066.

. Yen GC, Duh PD, Tsal CL.

Relationship between antioxidant

activity and maturity of peanut hulls.

J Argic Food chem.. 1993; 41: 67-

. Yildirim A, Mavi A, Kara AA.

Determination of antioxidant and

antimicrobial activities of Rumex

crispus L. extracts. Journal of

Agricultural and Food Chemistry.

; 9: 4083-4089.

. Zhao GR, Xiang ZJ, Ye TX. Yuan

YJ. Guo ZX. Antioxidant activities of

Salvia miltiorriza and Panax

notoginsing. Food Chem. 2006; 99:

-774.

. Zhishen J, Mengcheng T, Jianming

W. Research on antioxidant activity

of flavonoids from natural materials.

Food Chem. 1999; 64: 555-559.

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Published

30-09-2012

How to Cite

1.
Saradha .M, S. Paulsamy. n vitro antioxidant activity and polyphenol estimation of methanolic extract of endangered medicinal tree species, Hildegardia populifolia (Roxb.) Schott & Endl. ijp [Internet]. 2012 Sep. 30 [cited 2024 Dec. 6];4(3):362-8. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/187

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