Effect of rhizome extract of Acorus calamus on depressive condition induced by forced swimming in mice

Authors

  • Ilaiyaraja N Biochemistry and Nutrition Discipline Defence Food Research Laboratory, Siddhartha Nagar, Mysore-570011, India
  • Dongzagin Singsit Biochemistry and Nutrition Discipline Defence Food Research Laboratory, Siddhartha Nagar, Mysore-570011, India
  • Farhath Khanum Biochemistry and Nutrition Discipline Defence Food Research Laboratory, Siddhartha Nagar, Mysore-570011, India

Keywords:

Forced swimming test, Cortisol, Neurotransmitters, Monoamine oxidase

Abstract

The present study evaluated the anti-depressant properties of A. calamus rhizome in a forced swimming test (FST) of mice model. Three doses of methanol extract of rhizome (200,400 and 600 mg extract/kg b.wt) and imipramine (15 mg/kg b.wt), a positive control, were orally administered once a day for the consecutive period of 14 days in Balb/c mice. The effect of extract on immobility period was measured using forced swimming test. The levels of cortisol monoamine oxidase and neurotransmitters were analyzed using standard methods. The anti-depressant effect was observed maximum at the dose of 200 mg/kg. b.wt that caused 23.82% reduction in immobility period. The extract also significantly attenuated the FST-induced elevation of plasma cortisol, monoamine oxidase activity and returned the altered levels of neurotransmitters near to the normal levels in brain. These results of the present study suggest that the extract of A. calamus rhizome has antidepressant-like activity which is mediated by modulating the central neurochemical as well as HPA (hypothalamicpituitary-adrenal) axis in response to stress induced by FST. Therefore, A.calamus rhizome may be used as a valuable herbal supplement for the treatment of depression related conditions.

References

. Anisman, H, Zacharko RM.

Depression. The predisposing

influence of stress. Behav Brain Sci

; 5: 89-137.

. Chuang C, Shi Y, You H, Lo Y, Pa T.

Antidepressant effect of GABA-rich

monascus-fermented product on

forced swimming rat model. J Agric

Food Chem 2011; 59: 3027-3034.

. Xu Q, Pan Y, Li Y, Mo S, Jiang F,

Qiao C, Xu H, Lu X, Kong L, Kung

H. Anti-depressant-like effects of

psoralen isolated from the seeds of

Psoralea corylifolia in the mouse

forced swimming test. Biol Pharm

Bull 2008; 31: 1109-1114.

. Sánchez-Mateo CC, Prado CXB,

Rabanal RM. Antidepressant activity

of some Hypericum reflexum L. fil

extracts in the forced swimming test

in mice.J Ethnopharmacol 2007;

: 115-121.

. Rojas P, Serrano-García N, MedinaCampos ON, Pedraza-Chaverri J,

Ögren SO, Rojas C. Antidepressantlike effect of a Ginkgo biloba extract

(EGb761) in the mouse forced

swimming test: Role of oxidative

stress. Neurochem (International)

; 59: 628-636.

. Xia X, Cheng G, Pan Y, Xia ZH, Kong

LD. Behavior, neurochemical and

neuroendocrine effects of the

ethanolic extract from Curcuma

langa L in the mouse forced

swimming test. J Ethanopharmacol

; 110: 356-363.

. Yende SR, Harle UN, Rajgure DT,

Tuse TA, Vyawahare NS.

Pharmacological profile of Acorus

calamus: An Overview. Pharmacog

Reviews 2009; 2: 22-26.

. Palani S, Raja S, Praveen Kuma R,

Parameswaran P, Senthil Kumar B.

Therapeutic efficacy of Acorus

calamus on acetaminophen induced

nephrotoxicity and oxidative stress

in male albino rats. Acta

Pharmaceutica Sciencia 2010; 52:

-100.

. Parab RS, Mengi SA. Hypolipidemic

activity of Acorus calamus L. in rats.

Fitoterapia 2002; 73: 451-455.

. Muthuraman A, Singh N, Jaggi AS.

Protective effect of Acorus calamus

L. in rat model of vincristine induced

painful neuropathy: An evidence of

anti-inflammatory and anti-oxidative

activity. Food Chem Toxicol 2011;

: 2557-2563.

. Kim H, Han T, Lee S. Antiinflammatory activity of a water

extract of Acorus calamus L. leaves

on keratinocyte HaCaT cells. J

Ethnopharmacol 2009; 122: 149-

. Sandeep D, Nair CKK. Protection of

DNA and membrane from γ-

radiation induced damage by the

extract of Acorus calamus Linn.: An

in vitro study. Enl Toxicol Pharmacol

; 29: 302-307.

. Lee M, Chen Y, Tsai J, Wang S,

Watanabe T and Tsai Y. Inhibitory

effect of β-asarone, a component of

Acorus calamus essential oil, on

inhibition of adipogenesis in 3T3-L1

cells. Food Chem 2011; 126: 1: 1-7.

. Si M, Lou J, Zhou C, Shen J, Wu H,

Yang B, He Q, Wu H. Insulin

releasing and alpha-glucosidase

inhibitory activity of ethyl acetate

fraction of Acorus calamus in vitro

and in vivo. J Ethnopharmacol 2010;

: 154-159.

. Vohora, SB, Shah, SA, and

Dandiya, PC. Central nervous

system studies on an ethanol extract

of Acorus calamus rhizomes. J

Ethnopharmacol 1990; 28: 53-62.

. Shukla PK, Khanna V.

Neuroptotective effect of Acorus

calamus against middle cerebral

artery occlusion-induced ischaemia

in rats. Human Exp Toxicol 2006; 5:

-194.

. Mazza G. Gas chromatographic and

mass spectrometric studies of the

constituents of the rhizome of

calamus : I. The volatile constituents

of the essential oil. J

Chromatography A, 1985; 328: 179-

. Ilaiyaraja N, Farhath Khanum.

Amelioration of alcohol induced

hepatotoxicity and oxidative stress

in rats by Acorus calamus. J Diet

Suppl 2011; 8: 331-345.

. Porsolt RD, Bertin A, Jalfre M.

Behavioural despair in mice. A

primary screening test for

antidepressant. Arch Int

Pharmacodyn Ther 1977; 229: 327-

. Alburges ME, Narang N, Wamsley

JK. A sensitive and rapid HPLCECD method for the simultaneous

analysis of norepinephrine,

dopamine, serotonin and their

primary metabolites in brain tissue.

Biomed Chromatogr 1993; 7: 306-

. Schurr A, Livne A. Different

inhibition of mitochondrial

monoamine oxidase from brain by

hashish components. Biochem

Pharmacol 1976; 25: 1201-1203.

. Yu ZF, Kong LD, Chen Y.

Antidepressant activity of aqueous

extracts of Curcuma longa. J

Ethanopharmacol 2002; 83: 161-

. Lucki, I.The forced swimming test as

a model for core and component

behavioral effects of antidepressant

drugs. Behav Pharmacol 1997; 8:

-532.

. Dhingra D, Sharma A. Evaluation of

antidepressant-like activity of

glycyrrhizin in mice. Ind J Pharmacol

; 37: 390-394.

. Butterweck V, Nishibe S, Sasaki T,

Uchida M. Antidepressant effects of

Apocynum ventum leaves in a

forced swimming test. Biol Pharm

Bull 2001; 20: 848-851.

. Nakazaw T, Yasuda T, Ueda J,

Ohsawa K. Antidepressanat-like

effects of apigenin and 2, 4, 5-

trimethoxycinnamic acid from Perilla

frutescens in the forced swimming

test. Biol Pharm Bull 2003; 26: 474-

. Hill MN, Brotto LA, Lee TT and

Gorzalka B. Corticosterone

attenuate the antidepressant-like

effects elicited by melatonin in the

forced swim test in both male and

female

rats.Prog.Neuropsycopharmacol

Biol Psychiatry 2003; 27: 905-911.

. Pan Y, Kong L, Xia X, Zhang W, Xia

Z, Jiang F. Antideprssant-like effect

of icariin and its possible

mechanism in mice. Pharmaco

Biochem Behav 2005; 82: 686-694.

. Chen Y, Kong L, Xia X, Kung H,

Zhang L. Behavioral and

biochemical studies of total

furocoumarins from seeds of

Psoralea corylifolia in the forced

swimming test in mice

J.Ethanopharmacol 2005; 96: 451-

. Leem SA, Homh SS, Han XX,

Hwang JS, Oh GJ, Lee KS, Lee MK,

Hwang BY and Ro JS. Piperine from

the fruits of Piper longum with

inhibitory effects on monoamine

oxidase and antidepressant-like

activity. Chem Pharm Bull 2005; 53:

-835.

. Connor TJ, Kellliher P, Shen Y,

Harkin A, Kelly JP, Leonard BE.

Effect of subchronic antidepressant

treatments on behavioral,

neurochemical, and endocrine

changes in forced-swimming test.

Pharmacol Biochem Behav 2000;

: 591-7.

. Yi L, Li C, Zhan X, Cui C, Xiao F,

Zhou L, Y Xie Y. Involvement of

monoaminergic system in the

antidepressant-like effect of the

flavonoid naringenin in mice Prog

Neuropsychopharmacol Biol

Psychiatry 2010; 34: 1223-1228.

. Pandy V, Subhash H. CNS activity

of methanol and acetone extracts of

Acorus calamus leaves. J

Pharmacol Toxicol 2009; 4: 79-86.

. Hwang JH, Hong SS, Han XH,

Hwang JS, Lee D, Lee H, Yun YP,

Kim Y, Ro,JS, Hwang BY.

Prenylated xanthones from the root

bark of Cudrania tricuspidata. J Nat

Prod 2007; 70:1207-1209.

. Lee SJ, Chung HY, Lee IK, Oh SU

and Yoo ID. Phenolics with inhibitory

activity on mouse brain monoamine

oxidase (MAO) from whole parts of

Atemisia vulgaris L (Mugwort). Food

Sci Biotechnol 2000; 9: 179-182.

. Jeong SH, HanXH, Hong SS,

Hwang JS, Hwang JH, Lee D, Lee

MK, Ro JS, Hwang BY. Monoamine

oxidase inhibitory coumarins from

the aerial parts of Dictamnus albus.

Arch Pharm Res 2006; 29: 1119-

. Zhou Y, Shen Y, Zhang C, Su J, Liu

R, Zhang W. Triterpene saponins

from Bacopa monnieri and their

antidepressant effects in two mince

models. J Nat Prod 2007; 70:652-

. Yun B, Lee I, Ryoo I, Yoo I.

Coumarins with monoamine oxidase

inhibitory activity and antioxidative

coumarino-lignans from Hibiscus

syriacus. J Nat Prod 2001; 64 :

-1240.

. Xu Y, Li S, Chen R, Li G, Barish PA,

You W, Chen L, Lin M, Ku B, Pan J,

Ogle WO. Antidepressant-like effect

of low molecular proanthocyanidin in

mice: Involvement of

monoaminergic.system. Pharm

Biochem Behav 2010a; 94: 447-453.

. Machado DG, Bettio, LEB, Cunha

MP, Santos ARS, Pizzolatti MG,

Brighente IMC, Rodrigues ALS.

Antidepressant-like effect of rutin

isolated from the ethanolic extract

from Schinus molle L. in mice:

Evidence for the involvement of the

serotonergic and noradrenergic

systems. Eur J Pharmacol 2008;

: 1-3, 10: 163-168.

. Xu Y, Wang Z, You W, Zhang X, Li

S, Barish P, Vernon MM, Du X, Li G,

Pan J, William WO. Antidepressantlike effect of trans-resveratrol:

Involvement of serotonin and

noradrenaline system. Eur

Neuropsychopharmacol 2010b; 20:

-413.

. Takeda H, Tsuji M, Inazu M,

Egashira T, Matsumiya T.

Rosmarinic acid and caffeic acid

produce antidepressive-like effect in

the forced swimming test in mice.

Eur J Pharmacol 2002; 449: 3, 261-

.

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Published

30-09-2012

How to Cite

1.
Ilaiyaraja N, Dongzagin Singsit, Farhath Khanum. Effect of rhizome extract of Acorus calamus on depressive condition induced by forced swimming in mice. ijp [Internet]. 2012 Sep. 30 [cited 2024 Dec. 6];4(3):319-25. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/181

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