Phytochemical analysis and screening of antibacterial activity of some selected Indian medicinal plants
Keywords:
Medicinal Plants, bacterial strain, phytochemical screeningAbstract
Phytochemical screening and evaluation of antibacterial activity of ten plant species namely Aloe barbadensis, Aegel marmelos, Azadirachta indica, Bahunia variegate, Cannabis sativa, Emblica officinalis, Eugenia jambolana, Gmelina arborea, Nerium oleander and Vitex negundo have been carried out against six bacterial strains namely Escherichia coli 101, Escherichia coli 119, Micrococcus luteus, Bacillus subtilus, Bacillus cereus and Pseudomonas fluorescence. All the plants species taken in present study have shown the presence of tannin, flavonoids, terpenoids and alkaloids. Pholobatanins have found to be present in Aegel marmelos, Bahunia variegate and Eugenia jambolana while cardiac glycosides have been shown positive test on Aloe barbadensis, Azadirachta indica, Cannabis sativa, Nerium oleander and Vitex negundo. Methanol extract of Emblica officinalis have been shown significance potential against all test pathogens except Pseudomonas fluorescence followed by Nerium oleander, Azadirachta indica and Bahunia variegate.
References
. Thomson WAR. (Ed.), Medicines
from the Earth, McGraw-Hill Book
Co, Maidenhead, United Kingdom,
, 155.
. Stockwell C. Nature’s Pharmacy,
Century Hutchinson Ltd., London,
United Kingdom, 1988.
. Edeoga1 HO, Okwu DE, Mbaebie
BO. Phytochemical constituents of
some Nigerian medicinal plants Afr J
Biotech., 2005; 4, 685-688.
. Satish S, Raghavendra MP,
Raveesha KA. Evaluation of the
Antibacterial Potential of Some
Plants Against Human Pathogenic
Bacteria, Advances in Biological
Research, 2008; 2 (3-4): 44-48.
. Ceylan E, Fung DYC. Antimicrobial
activity of spices. J Rap Meth Auto
Microbiol 2004; 12: 1-55.
. Marjorie C. Plant products as
antimicrobial agents, Clinical
Microbiology Reviews, 1999; 12,
-582.
. DMPRD (Division of Medical Plants
Research and Development,
Department of Medical Science,
Ministry of Public Health). Hand
Book of Medicinal Plant for Primary
Public Health. Text and Journal
Corporation Co., Ltd. Press,
Bangkok. 1990; p. 53.
. Duke JA. Handbook of
Phytochemical Constituents of
GRAS Herbs and Other Economic
Plants. CRC Press, London, UK,
. Hon-Ning L. Chinese Medicinal
Herbs of Hong Kong, vol. 2. Hang
Chiewing Sa Kwang, Hong Kong,
. Husain A, Virmani OP, Popli SP.
Dictionary of Indian Medicinal
Plants. CIMPA, Lucknow, Republic
of India, 1992.
. Harbone JB. Phytochemical Method:
A Guide to Modern Technique of
Plant Analysis (2nd Edn), Chapman
and Hall, London, 1984; 19, 37 –
. Ahmad J, Mehmood Z, Mohammad
F. Screening of some Indian
medicinal plants for their
antimicrobial properties. J
Ethnopharmacol, 1998; 62, 183-
. Jamil A, Shahid M, Khan M, Ashraf
M. Pak J Bot., 2007; 39(1), 211-222.
. Samy RP. Antimicrobial activity of
some medicinal plants from India,
Fitoterapia, 2005; 76, 697-99.
. Parekh J, Karathia N, Chanda S.
Evaluation of antibacterial activity
and phytochemical analysis of
Bauhinia variegata L. bark, African
Journal of Biomedical Research,
; 9: 53 – 56.
. Appendino G, Gibbons S, Giana A,
Pagani A, Grassi G, Stavri M, Smith
E, Rahman MM. Antibacterial
Cannabinoids from Cannabis sativa:
A Structure—Activity Study, J. Nat.
Prod., 2008; 71(8), 1427-30.
. Saeed S, Tariq P, Antibacterial
activities of Emblica officinalis and
Coriandrum sativum against gram
negative urinary pathogens, Pak J
Pharm Sci., 2007; 20(1), 32-35.
. Zaika LL. Spices and herbs: their
antimicrobial activity and its
determination, J Food Safety., 1975;
: 97-118.
. Nair R, Kalariya T, Chanda S.
Antibacterial activity of some
selected Indian medicinal flora, Turk
J Biol., 2005; 29: 1-7.
. Arora C, Vashali, Synergistic effect
of antimicrobial constituents of Vitex
negundo with metal ferrocyanides,
Progressive Agriculture: An
International Journal, 2009; 9(1): 30-
. Adome RA, Gachihi JW. Onegi B,
Tamale J. Apio SO. The cardiotonic
effect of the crude ethanolic extract
of Nerium oleander in the isolated
guinea pig hearts, African Health
Science, 2003; 3(2): 77-82.
. Krishnaiah D, Devi T, Bono A,
Sarbatly R. Studies on
Phytochemical Constituents of Six
Malaysian medicinal plants, Journal
of Medicinal Plants Research,
; 3(2): 067-072.
. Perez C, Pauli M, Bazerque P. An
antibiotic assay by agar-well
diffusion method. Acta Biol Med Exp
;15:113-5.
. Awadh Ali NA, Juelich WD, Kusnick
C, Lindequist U. Screening of
Yemeni medicinal plants for antibacterial & cytotoxic activities. J
Ethnopharmacol, 2001; 74, 173-79.
. Kaushik RD, Garg GK, Sharma G,
Arora C. Antibacterial activity of
plant extracts from Uttaranchal Hills,
India. Allelopathy Journal, 2003,
(2), 205-214.