Effect of Withania somnifera leaf extracts as antibacterial agent against multidrug resistant bacteria

Authors

  • Rinku K. Chandoria Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, India- 474005
  • Hotam Singh Chaudhary Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, India- 474005
  • Alka Yadav Birla Institute of Medical & Research, Gwalior- India.
  • Abhinav Gupta Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, India-474005
  • Jay Prakash Yadav Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, India-474005
  • Anju Rawat Shrivastava Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, India-474005

Keywords:

Withania somnifera, Antibacterial activity, multi-drug resistance, IR spectral

Abstract

Objective: This study was designed to examine the phytochemical composition and to evaluate antibacterial potential of Withania Somnifera leaf against multidrug resistant bacteria & FT-IR Spectra of different bioactive compounds. Materials and Methods: Toluene, ethanol, and aqueous extracts of W. Somnifera (leaves) were subjected for in vitro antibacterial activity using agar well diffusion against different bacteria of clinical relevance including multi-drug resistant methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococcus (VRE) sps. Results: The toluene extract resulted maximal active as antibacterial agent and found significantly higher active than aqueous extract (P<0.05). The difference between antibacterial activities of toluene and ethanol or ethanol and aqueous extract was statistically non significant (P>0.05). The maximum activity was observed against Staphylococcus aureus. The extracts (at least one) also showed antibacterial activity against Bacillus cereus, coagulase negative staphylococcus, Streptococcus pneumoinae, Shigella dysenteriae, Methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococcus (VRE) sps. Minimum inhibitory concentrations of plant extract were found in the ranges of 0.156mg/ml to 0.625mg/ml for test strains. Phytochemical analyses revealed the presence of various metabolites like phlobtannins, tannins, steroids and alkaloids which may contribute for the antimicrobial action of leaves extract of W. Somnifera. The partial characterization of the crude extracts by IR spectral analysis revealed the possible presence of different bioactive compounds in the extracts. Conclusion: The leaves of W. Somnifera showed promising antibacterial activity against various clinically important bacteria and multidrug resistant MRSA and VRE

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Published

30-06-2012

How to Cite

1.
Rinku K. Chandoria, Hotam Singh Chaudhary, Alka Yadav, Abhinav Gupta, Jay Prakash Yadav, Anju Rawat Shrivastava. Effect of Withania somnifera leaf extracts as antibacterial agent against multidrug resistant bacteria. ijp [Internet]. 2012 Jun. 30 [cited 2024 Dec. 6];4(2):237-42. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/168

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Original Research Articles