Antimicrobial Activity of Lemongrass and Oregano essential oil against standard antibiotic resistant Staphylococcus aureus and field isolates from chronic mastitis cow
Keywords:
Lemongrass, Oregano essential oil, Resistan, S. aureus, antibioticsAbstract
The study was carried out to investigate the antibacterial activity of lemongrass and Oregano essential oil against standard antibiotic resistant Staphylococcus aureus strains and field isolates from a chronic mastitic cow. Disc diffusion and minimum inhibitory concentration (MIC) assay were used to investigate antibacterial activity of lemongrass and Oregano essential oil while their combined effect with antibiotic were assessed using a microbroth chequerboard technique. For identification of the essential oil component Gas Chromatography Coupled Mass Spectroscopy (GC/MS) analysis was performed. Disc diffusion assay revealed dose-dependent inhibition zone with lemongrass oil showing consistently larger inhibition zone than oregano oil. Lemongrass essential oils were further evaluated for its antibacterial activity in combination with antibiotic which resulted both synergistic and additive interaction. Lemongrass oil-amoxicillin combination showed synergistic interactions against all tested organism while the combination for lemongrass oilnorfloxacin showed synergistic effect for two field isolates and an additive for the remaining strains. The current study indicated that both lemongrass and oregano essential oils exhibit antibacterial activity, and lemongrass oil in combination with antibiotics could be a potential agent for antibacterial treatments against antibiotic resistant bacterial infections.
References
. Helander IK, Alakomi HL, Latva-Kala
K, Mattila-Sandholm T, Pol I, Smid EJ,
von Wright A. Characterization of the
action of selected essential oil
components on Gram negative
bacteria. J Agric Chem.
;46:3590–3595.
. Chorianopoulos N, Kalpoutzakis E,
Aligiannis N, Mitaku S, Nychas GJ,
Haroutounian SA. Essential oils of
Satureja, Origanum, and Thymus
species: chemical composition and
antibacterial activities against
foodborne pathogens. J Agric Food
Chem. 2004;52:8261-7.
. Si H, Hu J, Liu Z, Zeng ZL.
Antibacterial effect of oregano
essential oil alone and in combination
with antibiotics against extendedspectrum b-lactamase-producing
Escherichia coli. FEMS Immunol Med
Microbiol. 2008;53:190-4.
. Melter O, Aires de Sousa M,
Urbaskova P, Jakubu V, Zemlickova
H, de Lencastre H. Update on the
major clonal types of methicillinresistant Staphylococcus aureus in
the Czech Republic. J Clin Microbiol.
;41:4998-5005.
. Lee JH. Methicillin (Oxacillin)-resistant
Staphylococcus aureus strains
isolated from major food animals and
their potential transmission to
humans. Appl Environ Microbiol.
;69:6489-94.
. Kobayashi H. National hospital
infection surveillance on methicillinresistant Staphylococcus aureus. J
Hosp Infect. 2005;60:172-5.
. Tenover FC, Biddle JW, Lancaster
MV. Increasing resistance to
vancomycin and other glycopeptides
in Staphylococcus aureus. Emerg
Infect dis. 2001;7:327–32.
. Barnard FM, Maxwell A. Interaction
between DNA gyrase and quinolones:
effects of alanine mutations at GyrA
subunit residues Ser(83) and Asp(87).
Antimicrob Agents Chemother.
;45(7):1994-2000.
. Ouattara B, Simard RE, Holley RA,
Piette GJ, Bégin A. Antibacterial
activity of selected fatty acids and
essential oils against six meat
spoilage organisms. Int J Food
Microbiol. 1997;37:155-62.
. Prabuseenivasan S, Jayakumar M,
Ignacimuthu S. In vitro antibacterial
activity of some plant essential oils.
BMC Complement Altern Med.
;6:39.
. V´agi E, Sim´andi B, Suhajda A,
H´ethelyi E. Essential oil composition
and antimicrobial activity of Origanum
majorana L. extracts obtained with
ethyl alcohol and supercritical carbon
dioxide. Food Res Int. 2005;38:51–57.
. Tohidpour A, Sattari M, Omidbaigi R,
Yadegar A, Nazemi J. Antibacterial
effect of essential oils from two
medicinal plants against Methicillinresistant Staphylococcus aureus
(MRSA). Phytomedicine.
;17:142-5.
. Jayaraman P, Sakharkar MK, Lim CS,
Tang TH, Sakharkar KR. Activity and
interactions of antibiotic and
phytochemical combinations against
Pseudomonas aeruginosa in vitro. Int
J Biol Sci. 2010;6:556-68.
. Burt SA. Essential oils: their
antibacterial properties and potential
applications in foods: a review. Inter J
Food Microbiol. 2004;94:223-253