Development, characterization, efficacy and repeated dose toxicity of nanoemulsified ethanolic extract of Enicostemma littorale in Streptozotocin-induced diabetes rats.

Authors

  • Deepa V International Institute of Biotechnology and Toxicology, Padappai, Kanchipuram district, India.
  • Sridhar R International Institute of Biotechnology and Toxicology, Padappai, Kanchipuram district, India.
  • Neelakanta Reddy P Bio-organic Chemistry Laboratory, Central Leather Research Institute, Adayar, Chennai, India
  • Balakrishna Murthy P International Institute of Biotechnology and Toxicology, Padappai, Kanchipuram district, India.

Keywords:

nanoemulsion, streptozotocin, diabetics, anti-diabetic, Enicostemma littorale

Abstract

Alginate nanocapsules of ethanolic extract of Enicostemma littorale (NEL) were prepared by emulsification, cross linking with calcium chloride and solvent removal. Based on total phenol content the loading efficiency of the nanocapsules was 89% at an optimum concentration of 2: 18 (mg ml -1) for plant extract: olive oil. The photon correlation spectroscopy (PCS) revealed that the mean particle diameter of optimized formulation was 233 nm and scanning electron microscopy (SEM) showed a spherical morphology. When subjected to Fourier transform infrared spectroscopy (FT-IR) for the compatability analysis between plant extract and sodium alginate, it revealed that the phytoconstituents were stable. The purpose of the present study was to compare the anti-diabetic activity of NEL and E.littorale (EL) in streptozoticin induced male rats. An oral dose of NEL (20 mg/kg b.w) and EL (2000 mg/kg b.w) showed a relatively similar antidiabetic effect, reducing the blood glucose, triglycerides, cholesterol, creatinine, ALT, AST, and ALP. Moreover, NEL is 100 times less than EL exhibiting better results within 10 days of treatment. These biochemical assessments were supported by rat biopsy examinations. In conclusion, the nanoemulsification method can be applied for poor water-soluble ethanolic herbal extracts to reduce the dosage and time

References

. Vallabhji J., McColl A.J., Richmond

W., Schachter M., Rubens M.B.,

Elkeles R.S., Total antioxidant status

and coronary artery calcification in

type 1 diabetes. Diabetes

Care.,2001, 24: 1608–1613.

. Zhang X.F., Tan B.K.,

Antihyperglycaemic and antioxidant

properties of andrographis

particulate in normal and diabetic

rats. Clin. Exp. Pharmacol., 2000,

: 5–6.

. Skim F., Lazrek H.B., Kaaya A.,

Elamri H., Jana M., Pharmacological

studies of two antidiabetic plants:

Globularia alypum and Zygophyllum

gaetulum. Therapia., 1999, 54:711–

. Reeves W.B., Andreoli T.E.,

Transforming growth factor-h

contributes to progressive diabetic

nephropathy. Proccedings National

Academic Science, U S A., 2000, 97:

–9.

. Mauer S.M., Steffes M.W., Ellis E.N.,

Sutherland D.E., Brown D.M., Goetz

F.C., Structural–functional

relationships in diabetic

nephropathy. Journal of Clinical

Investment., 1984, 74: 1143– 55.

. Mauer S.M., Lane P., Hattori M.,

Fioretto P., Steffes M.W., Renal

structure and function in insulindependent diabetes mellitus and

type I membranoproliferative

glomerulonephritis in humans.

Journal of Americn Society of

Nephrology., 1992, 2:S181–4.

. Lu M.F., Cheng Y.Q., Li L.J., Wu

J.J., Progress of study on passive

targeting of drug delivery system.

Material Review., 2005, 19: 108–

21

. Sonawane R.D., Viswakarma S.L..,

Lakshmi S, Rajini M., Padh H., Goyal

R.K., Amelioration of STZ – induced

type-1 diabetic nephropathy by

aqueous extract of Enicostemma

littorale Blume and swertiamarin in

rats. Molecular Cell

Biochemistry.,2010, 340: 1-6.

. Bhattacharya S., Phytosomes:

Emerging Strategy in Delivery of

Herbal Drugs and Nutraceuticals,

Pharma Times. 2009, 41: 9–12

Downloads

Published

31-03-2012

How to Cite

1.
Deepa V, Sridhar R, Neelakanta Reddy P, Balakrishna Murthy P. Development, characterization, efficacy and repeated dose toxicity of nanoemulsified ethanolic extract of Enicostemma littorale in Streptozotocin-induced diabetes rats. ijp [Internet]. 2012 Mar. 31 [cited 2024 Dec. 23];4(1):70-89. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/148

Issue

Section

Original Research Articles