Standardization of Euphorbia hirta with Chemical Compounds Identification (GC-MS)

Authors

  • Kwan Yuet Ping Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
  • Ibrahim Darah School of Biological Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
  • Umi Kalsom Yusuf Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Lachimanan Yoga Latha Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
  • Sreenivasan Sasidharan Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia

Keywords:

E. hirta, FTIR fingerprints, heavy metals, microscopical, macroscopical standardization

Abstract

The present study was intended to evaluate the various pharmacognostical procedures in the leaves of E. hirta, (Euphorbiaceae). The various pharmacognostical parameters were carried out as per WHO guidelines procedure i.e., macroscopic and microscopic evaluation, heavy metal analysis, functional group analysis by Fourier Transform Infrared (FTIR) spectroscopy and assay. The study was extended with analyzing the chemical compounds identification in the extract of E. hirta by using GC-MS. The assay part of standardization involved determination of the antioxidant activity (DPPH assay) which could help assesses the chemical effects and establishes curative values. The extracts showed an antioxidant activity above 50%, independent of the extraction time. Six major peaks in the range of 600 - 1500 and 2800 - 3400 cm-1 were observed in the FTIR spectra. The chemical constituent aspect of standardization involves quantification of the main chemical components in E. hirta. The GC-MS method used for quantification of 1,3,4,5-tetrahydroxycyclohexanecarboxylic acid in the extract was rapid, accurate, precise, linear (R2 = 0.9981), rugged and robust. The concentrations of heavy metals determined in E. hirta extract are well below the permissible limit. The result of the pharmacognostical standardization of this plant serves as a reference piece and helps in future identification and authentication of this plant specimen.

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Published

31-03-2012

How to Cite

1.
Kwan Yuet Ping, Ibrahim Darah, Umi Kalsom Yusuf, Lachimanan Yoga Latha, Sreenivasan Sasidharan. Standardization of Euphorbia hirta with Chemical Compounds Identification (GC-MS). ijp [Internet]. 2012 Mar. 31 [cited 2024 Dec. 23];4(1):12-21. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/142

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