Preliminary phytochemical analysis & Invitro Antibacterial activity of Acacia catechu willd Bark against Streptococcus mitis, Streptococcus sanguis & Lactobacillus acidophilus.
Keywords:
Acacia catechu, Anti bacterial activity, oral microbes, MBC, PhytochemicalsAbstract
The objective of our study is to investigate the in vitro antibacterial activity of ethanolic bark extract of Acacia catechu willd against selected oral microbes and to investigate the phytochemicals present in it. The inhibitory effect of the extract were tested against selected oral microbes by using the Macro broth dilution method .The ethanolic bark extract of Acacia catechu exhibited antibacterial activity against streptococcus mitis with minimum bactericidal concentration of 500µg/ml whereas the MBC for streptococcus sanguis and Lactobacillus acidophilus were found to be 1mg/ml, 5mg/ml,10mg/ml and the phytochemical analysis revealed the presence of tannins, flavonoids, amino acids , saponins, triterpenoids. The ethanolic bark extract of Acacia catechu willd was found to be bactericidal in action against tested bacterial strains and these action may be due to the presence of phytochemical constituents in it.
References
. Anonymous, Indian Herbal Pharmacopoeia,
Indian Drug Manufacturer’s Association,
Mumbai, 2002, 1-11.
. Singh KN, Lal B, Note on traditional uses of
Khair (Acacia catechu Willd.) by inhabitants of
shivalik range of western Himalaya,
Ethnobotanical Leaflets, 10, 2006, 109-112.
. Qadry JS, Shah’s and Qadry’s Pharmacognosy,
th edition, B.S Shah Prakashan, Ahmedabad,
, 302-303.
. Lakshmi.T, Geetha R.V, Anitha Roy. In vitro
Evaluation of Anti bacterial Activity of Acacia
catechu willd Heartwood Extract.” International
journal of Pharma and Biosciences. 2011;2(2);
B188-192.
. Geetha R.V, Anitha Roy, Lakshmi.T .In vitro
evaluation of Anti bacterial activity of heartwood
extract of Acacia catechu on oral microbes.
International journal of current research and
review .2011;3(6);P.4-9.
. Geetha R.V, Anitha Roy, Lakshmi T . In vitro
evaluation of anti bacterial activity of heart wood
extract of Acacia catechu willd on enteric
pathogens.International journal of pharmaceutical
sciences review and research.2011;9(2);P.147-
. Anitha Roy, Geetha R.V, Lakshmi T. In Vitro
Evaluation of Anti Mycotic Activity of
Heartwood Extract of Acacia Catechu Willd
.Journal of pharmacy research .2011;4(7)
. Naik GH, Priyadarsini KI, Satav JG, Banavalikar
MM, Sohoni DP, Biyani MK, Mohan H,
Comparative antioxidant activity of individual
herbal components used in Ayurvedic medicine,
(1), 2003, 97-104.
. Sharma P, Dayal R, Ayyer KS, Chemical
constituents of Acacia catechu leaves, Indian
Journal of Chemical Society, 1997;P.60.
. Rao PR, Seshadri TR; L-Epi-catechin from
Acacia catechu. J. Scient. Ind. Res., (7B); 59
;1948.
. Siddiqui S, Verma A, Rather AA, Jabeen F,
Meghvansi MK, Preliminary Phytochemicals
Analysis of Some Important Medicinal and
Aromatic Plants, Advances in Biological
Research, 3, 2009, 188-195.
. Edeoga HO, Okwu DE, Mbaebie BO,
Phytochemical constituents of some Nigerian
medicinal Plants, African Journal of
Biotechnology, 4, 2005, 685-688.
. Iyengar, M.A., 1995. Study of Crude Drugs.
th ed., Manipal Power Press, Manipal, India.
pp 2.
. Evans, W.C., 2002. Trease and Evan’s
Pharmacognosy. 5th ed., Haarcourt Brace And
Company, pp 336.
. Siddiqui, A.A., Ali, M., 1997. Practical
Pharmaceutical chemistry. Ist ed., CBS Publishers
and Distributors, New Delhi, pp 126-131.
. J.H Jorgenson & John turniegd
Susceptibility test methods dilution and disc
diffusion methods,Manual of Clinical
microbiology ASM Press Washington vol 1
,9th edition pg no.1153-1172.
. Betty A.Forbes., Daniel F.Sahm., Alice
S.Weissfeld. Bailey & Scott’s Diagnostic
Microbiology 11th edition Mosby page no 229 –
. Ananthanarayan R and Paniker‘s:
Textbook of Microbiology: 8th edition:
Publishers University Press: Hyderabad 2009:
. Jennifer MA: Determination of Minimum
Inhibitory Concentrations: Journal of
Antimicrobial Chemotherapy 2001; 48, (SI):
-16.
. National Committee for Clinical Laboratory
Standards: Methods for Disc Susceptibility Tests
for Bacteria that Grow Aerobically: NCCLS
Document M2-A7: National Committee for
Clinical Laboratory Standards: Wayne, USA:
;
. Rogers A H (editor). (2008). Molecular Oral
Microbiology. Caister Academic Press. ISBN
-1-904455-24-0.
. Anhoury P et al Microbial profile on
metallic and ceramic bracket materials. Angle
Orthodontist 2002;72:338-343.
. Bos R, van der Mei HC, Busscher HJ
Physico-chemistry of initial microbial
adhesive interactions—its mechanisms and
methods for study. FEMS Microbiology
Reviews 1999;23:179-230.
. Diamanti-Kipioti A, Gusberti FA, Lang
NP Clinical and microbiological effects of
fixed orthodontic appliances. Journal of
Clinical Periodontology 1987;14:326-333.
. Atack NE, Sandy jr ,Addy M Periodontal
and microbiological changes associated with
the placement of orthodontic appliances. A
review. Journal of Periodontology
;67:78-85.