Preliminary phytochemical analysis & Invitro Antibacterial activity of Acacia catechu willd Bark against Streptococcus mitis, Streptococcus sanguis & Lactobacillus acidophilus.

Authors

  • Lakshmi T Department of Pharmacology, Saveetha Dental College & Hospitals, Chennai.
  • Aravind kumar S Department of Orthodontics, Saveetha Dental College & Hospitals, Chennai.

Keywords:

Acacia catechu, Anti bacterial activity, oral microbes, MBC, Phytochemicals

Abstract

The objective of our study is to investigate the in vitro antibacterial activity of ethanolic bark extract of Acacia catechu willd against selected oral microbes and to investigate the phytochemicals present in it. The inhibitory effect of the extract were tested against selected oral microbes by using the Macro broth dilution method .The ethanolic bark extract of Acacia catechu exhibited antibacterial activity against streptococcus mitis with minimum bactericidal concentration of 500µg/ml whereas the MBC for streptococcus sanguis and Lactobacillus acidophilus were found to be 1mg/ml, 5mg/ml,10mg/ml and the phytochemical analysis revealed the presence of tannins, flavonoids, amino acids , saponins, triterpenoids. The ethanolic bark extract of Acacia catechu willd was found to be bactericidal in action against tested bacterial strains and these action may be due to the presence of phytochemical constituents in it.

References

. Anonymous, Indian Herbal Pharmacopoeia,

Indian Drug Manufacturer’s Association,

Mumbai, 2002, 1-11.

. Singh KN, Lal B, Note on traditional uses of

Khair (Acacia catechu Willd.) by inhabitants of

shivalik range of western Himalaya,

Ethnobotanical Leaflets, 10, 2006, 109-112.

. Qadry JS, Shah’s and Qadry’s Pharmacognosy,

th edition, B.S Shah Prakashan, Ahmedabad,

, 302-303.

. Lakshmi.T, Geetha R.V, Anitha Roy. In vitro

Evaluation of Anti bacterial Activity of Acacia

catechu willd Heartwood Extract.” International

journal of Pharma and Biosciences. 2011;2(2);

B188-192.

. Geetha R.V, Anitha Roy, Lakshmi.T .In vitro

evaluation of Anti bacterial activity of heartwood

extract of Acacia catechu on oral microbes.

International journal of current research and

review .2011;3(6);P.4-9.

. Geetha R.V, Anitha Roy, Lakshmi T . In vitro

evaluation of anti bacterial activity of heart wood

extract of Acacia catechu willd on enteric

pathogens.International journal of pharmaceutical

sciences review and research.2011;9(2);P.147-

. Anitha Roy, Geetha R.V, Lakshmi T. In Vitro

Evaluation of Anti Mycotic Activity of

Heartwood Extract of Acacia Catechu Willd

.Journal of pharmacy research .2011;4(7)

. Naik GH, Priyadarsini KI, Satav JG, Banavalikar

MM, Sohoni DP, Biyani MK, Mohan H,

Comparative antioxidant activity of individual

herbal components used in Ayurvedic medicine,

(1), 2003, 97-104.

. Sharma P, Dayal R, Ayyer KS, Chemical

constituents of Acacia catechu leaves, Indian

Journal of Chemical Society, 1997;P.60.

. Rao PR, Seshadri TR; L-Epi-catechin from

Acacia catechu. J. Scient. Ind. Res., (7B); 59

;1948.

. Siddiqui S, Verma A, Rather AA, Jabeen F,

Meghvansi MK, Preliminary Phytochemicals

Analysis of Some Important Medicinal and

Aromatic Plants, Advances in Biological

Research, 3, 2009, 188-195.

. Edeoga HO, Okwu DE, Mbaebie BO,

Phytochemical constituents of some Nigerian

medicinal Plants, African Journal of

Biotechnology, 4, 2005, 685-688.

. Iyengar, M.A., 1995. Study of Crude Drugs.

th ed., Manipal Power Press, Manipal, India.

pp 2.

. Evans, W.C., 2002. Trease and Evan’s

Pharmacognosy. 5th ed., Haarcourt Brace And

Company, pp 336.

. Siddiqui, A.A., Ali, M., 1997. Practical

Pharmaceutical chemistry. Ist ed., CBS Publishers

and Distributors, New Delhi, pp 126-131.

. J.H Jorgenson & John turniegd

Susceptibility test methods dilution and disc

diffusion methods,Manual of Clinical

microbiology ASM Press Washington vol 1

,9th edition pg no.1153-1172.

. Betty A.Forbes., Daniel F.Sahm., Alice

S.Weissfeld. Bailey & Scott’s Diagnostic

Microbiology 11th edition Mosby page no 229 –

. Ananthanarayan R and Paniker‘s:

Textbook of Microbiology: 8th edition:

Publishers University Press: Hyderabad 2009:

. Jennifer MA: Determination of Minimum

Inhibitory Concentrations: Journal of

Antimicrobial Chemotherapy 2001; 48, (SI):

-16.

. National Committee for Clinical Laboratory

Standards: Methods for Disc Susceptibility Tests

for Bacteria that Grow Aerobically: NCCLS

Document M2-A7: National Committee for

Clinical Laboratory Standards: Wayne, USA:

;

. Rogers A H (editor). (2008). Molecular Oral

Microbiology. Caister Academic Press. ISBN

-1-904455-24-0.

. Anhoury P et al Microbial profile on

metallic and ceramic bracket materials. Angle

Orthodontist 2002;72:338-343.

. Bos R, van der Mei HC, Busscher HJ

Physico-chemistry of initial microbial

adhesive interactions—its mechanisms and

methods for study. FEMS Microbiology

Reviews 1999;23:179-230.

. Diamanti-Kipioti A, Gusberti FA, Lang

NP Clinical and microbiological effects of

fixed orthodontic appliances. Journal of

Clinical Periodontology 1987;14:326-333.

. Atack NE, Sandy jr ,Addy M Periodontal

and microbiological changes associated with

the placement of orthodontic appliances. A

review. Journal of Periodontology

;67:78-85.

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Published

31-12-2011

How to Cite

1.
Lakshmi T, Aravind kumar S. Preliminary phytochemical analysis & Invitro Antibacterial activity of Acacia catechu willd Bark against Streptococcus mitis, Streptococcus sanguis & Lactobacillus acidophilus. ijp [Internet]. 2011 Dec. 31 [cited 2024 Jun. 12];3(4):579-84. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/139

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Original Research Articles