In Vitro Antioxidant Activity of Flowers and Fruits of Alstonia scholaris

Authors

  • Joel James School of Biosciences and Technology VIT University, Vellore-632014 Tamil Nadu, India
  • Arun Kumar Thaliyil Veettil School of Biosciences and Technology VIT University, Vellore-632014 Tamil Nadu, India
  • Kumar Pratyush School of Biosciences and Technology VIT University, Vellore-632014 Tamil Nadu, India
  • Chandra Shekhar Misra School of Biosciences and Technology VIT University, Vellore-632014 Tamil Nadu, India
  • Lipin Dev Mundur Sahadevan School of Biosciences and Technology VIT University, Vellore-632014 Tamil Nadu, India
  • Thankamani V School of Biosciences and Technology VIT University, Vellore-632014 Tamil Nadu, India.

Keywords:

Alstonia scholaris, anti oxidant potential, DPPH, Beta Carotene

Abstract

The ethnobotanical and pharmacological evaluation of plant based chemicals have shown rapid strides in the last few decades. Plants have been a rich source of important therapeutic agents and form the basis of herbal systems of medicine, like ayurveda, resulting in the revival of ancient traditions of medicine. The present study was carried out to investigate the anti oxidant potential of the inflorescence and fruits of Alstonia scholaris using an in vitro model system like DPPH assay and Beta carotene Assay. The methanol extract of the flower showed powerful antioxidant activity by DPPH and Betacarotene assays in comparison with the standard butylated hydroxy toluene (BHT), l- ascorbic acid. For DPPH assay the IC-50 value was also calculated and was found to have a significant correlation between benzene extract of flower and methanol extract of fruits. Overall, the methanol extracts of flower showed higher anti oxidant activity than the fruit

References

Newman DJ, Cragg GM, Snader KM. Natural products

as sources of new drugs over the period 1981-2002. J

Nat Prod. 2003;66:1022-1037.

Brand WW, Cuvelier HE, Berset C. Use of free radical

method to evaluate antioxidant activity. Food Sci

Technol. 1995;82:25-30.

Manjunatha BK and Vidya SM. Indian Journal of

Pharmaceutical Sciences. 2008;70:241.

Nadkarni AK. KM Nadkarni’s Indian Materia Medica,

Vol. 1, Bombay, India. Popular Prakashan. 1976;p. 80-

Kirtikar KR, Basu BD. Indian Medicinal Plants, Vol.1,

Allahabad, India. Lalit Mohan Basu. 2002;p. 111-14.

Singh MP, Panda H. Medicinal Herbs with Their

Formulations. Delhi: Daya Publishing house; 2005;p

-90.

Channa S, Dar A, Ahmed S, Rahman A. Evaluation of

Alstonia scholaris leaves for broncho-vasodilatory

activity. Journal of Ethnopharmacology. 2005;97:469-

Jagetia GC, Baliga MS. The evaluation of nitric oxide

scavenging activity of certain Indian medicinal plants in

vitro: a preliminary study. J Med Food. 2004;7:343 –

Blois MS. Antioxidant determination by the use of a

stable free radical. Nature. 1958;181:1199-1200.

Graven EH, Dean SG, Svoboda KP, Mavi S and

Gundidza MG. Antimicrobial and antioxidative

properties of the volatile (essential) oil of Artemisia afra

Jacq. Flavour and Fragrance journal. 1992;7:121-123.

Jagetia GC, Baliga MS. The evaluation of nitric oxide

scavenging activity of certain Indian medicinal plants in

vitro: a preliminary study. J Med Food. 2004;7:343 –

Arulmozhi.S, Mazumder PM, Sathiyanarayanan L,

Ashok P. Screening of Alstonia scholaris Linn. R.Br.,

for wound healing activity. Opem. 2007;7:254-260.

Ilhami Gulcin, Haci Ahmet Alici, Mehmet Cesur.

Determination of in vitro antioxidant and radical

scavenging activities of propofol. Chem Pharm Bull.

;53:281–285.

Wong C, Li H, Cheng K, Chen F. A systematic survey

of antioxidant activity of 30 Chinese medicinal plants

using the ferric reducing antioxidant power assay. Food

Chem. 2006;97:705711.

Soares JR, Dinis TCP, Cunha AP, Almeida LM.

Antioxidant activities of some extracts of Thymus zygis.

Free Radical Research. 1997;26:469 – 478.

Thankamani V, James J, Veettil AKT and Sagadevan

LDM. Phytochemical screening and anti microbial

activity of Alstonia scholaris flowers (L) R.BR. Fam:

Apocynaceae. International Journal Of Pharmaceutical

Research And Development 2011;3(3):172-178.

Mishra J,Yousuf A,Singh RD,Aradhana. Phytochemical

investigation and in-vitro antioxidant potential of leaves

of Murraya koenigii.International Jornal of Integrative

Biology. 2009;7(3):171-174

Kyselova Y, Ivanova D,et al. Correlation between the

invitro antioxidant activity and the polyphenol content of

the aqueous extracts from Bulgarian herbs.

Phytotherapy research. 2006;20:961-965.

Silva EM,Souza JNS,et al. Antioxidant activites and

polyphenolic contents of fifteen selected plants species

from the Amazonian region. Food Chem.

;101:1012-1018.

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Published

31-12-2011

How to Cite

1.
Joel James, Arun Kumar Thaliyil Veettil, Kumar Pratyush, Chandra Shekhar Misra, Lipin Dev Mundur Sahadevan, Thankamani V. In Vitro Antioxidant Activity of Flowers and Fruits of Alstonia scholaris. ijp [Internet]. 2011 Dec. 31 [cited 2024 Jun. 13];3(4):475-9. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/128

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Original Research Articles