Antidiabetic and hypolipidemic effects of mahanimbine (carbazole alkaloid) from murraya koenigii (rutaceae) leaves

Authors

  • B. Dineshkumar School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721 302, West Bengal, India
  • Analava Mitra School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721 302, West Bengal, India
  • Manjunatha Mahadevappa School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721 302, West Bengal, India.

Keywords:

Streptozotocin, Hypoglycemic, Hypolipidemic, Mahanimbine

Abstract

Murraya koenigii leaves (Rutaceae) are used traditionally in Indian Ayurvedic system to treat diabetes. The purpose of the study is to investigate the effect of mahanimbine (carbazole alkaloid from Murraya koenigii leaves) on blood glucose and serum lipid profiles on streptozotocin-induced diabetic rats. Diabetes was induced in adult male Wistar rats by intra-peritoneal injection of streptozotocin (45mg/kg). Mahanimbine (50 and 100mg/kg) were administrated as a single dose per week to the diabetic rats for 30 days. The control group received 0.3% w/v sodium carboxy methyl cellulose for the same duration. Fasting blood sugar and serum lipid profiles were measured in the diabetic and non-diabetic rats. In addition, in vitro alpha amylase and alpha glucosidase inhibitory effects of mahanimbine were performed. Results: In the diabetic rats, the elevated fasting blood sugar, triglycerides, low density lipoprotein, very low density lipoprotein levels were reduced and high density lipoprotein level was increased by mahanimbine at a dose of 50 and 100mg/kg (i.p). In addition, mahanimbine showed appreciable alpha amylase inhibitory effect and weak alpha glucosidase inhibitory effects when compared with acarbose. Conclusions: The present study indicated that mahanimbine possess anti-hyperglycemic and anti-lipidemic effects. Thus results suggesting mahanimbine has beneficial effect in the management of diabetes associated with abnormal lipid profile and related cardiovascular complications.

Author Biography

B. Dineshkumar, School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721 302, West Bengal, India

+91-3222-2882220

References

Mitra A. Some salient points in dietary and

life style of rural Bengal particularly tribal

populace in relation to rural diabetic

prevalence. Studies on Ethno-Med 2008, 2:

-56.

Fowler MJ. Diabetes Treatment, Part 2: Oral

agents for glycemic management. Clin

Diabetes 2007, 25: 131-134.

Mukherjee PK, Maitl K, Mukherjee K,

Houghton PJ. Leads from Indian medicinal

plants with hypoglycemic potentials. J

Ethnopharmacol 2006, 106: 1-28.

Valiathan MS. Healing plants. Curr Sci 1998,

: 1122.

Kong YC, Ng KH, But PP H, Li Q, Yu SX,

Zhang HT, Cheng KF, Soejarto DD, Kan WS,

Waterman PG. Sources of the antiimplanation alkaloid yuehchukene in the

genus Murraya. J Ethnopharmacol 1986, 15:

-200.

Keasri AN, Kesari S, Singh SK, Gupta RK,

Watal G. Studies on the glycemic and

lipidemic effect of Murraya koenigii in

experimental animals. J Ethnopharmacol

, 112: 305-311.

Knolker HJ, Reddy KR. Isolation and

synthesis of biologically active carbazole

alkaloids. Chem Rev 2002, 102: 4303-4427.

Knolker HJ, Reddy KR. Biological and

pharmacological activities of carbazole

alkaloids. The Alkaloids 2008, 65: 181-193.

Reisch J, Adeleke AC, Kumar V, Aladesanmi

AJ. Two carbazole alkaloids from Murraya

koenigii. Phytochem 1994, 36: 1073-1076.

Gupta RK, Kesari AN, Murthy PS, Chandra

R, Tandon V, Watal G. Hypoglycemic and

Antidiabetic effect of ethanolic extract of

leaves of Annona squamosa L. in

experimental animals. J Ethnopharmacol

, 99: 75-81.

Hansawasdi C, Kawabata J, kasai T. α-

amylase inhibitors from Roselle (Hibiscus

sabdariffa Linn.) tea. Biosci Biotechnol

Biochem 2000, 64: 1041-1043.

Pistia-Brueggeman G, Hollingsworth RI. A

preparation and screening strategy for

glycosidase inhibitors. Tetrahedron 2001, 57:

-8778.

Abu bakar NH, Sukari MA, Rahmani M,

Sharif AM, Khalid K, Yusuf UK. Chemical

consititutents from stem barks and roots of

Murraya koenigii (Rutaceae). Malays J Anal

Sci 2007, 11: 173-176.

Rao LJM, Ramalakshmi K, Borse BB,

Raghavan B. Antioxidant and radicalscavenging carbazole alkaloids from the

oleoresin of curry leaf (Murraya koenigii).

Food Chem 2007, 100: 742-747.

Temme Eh, Vaqn HPG, Schouten EG,

Kesteloot H. Effect of plant sterol-enriched

spread on serum lipids and lipoproteins in

middly hypercholesterolaemic subjects. Acta

Cardiol 2002, 57: 111-115.

Bainton D, Miller NE, Botton CH, Yarnell

JWG, Suretman PM, Baker IA. Plasma

triglycerides and high density lipoprotein

cholesterol as predictors of ischemic heart

disease in British man. Br Heart J 1992, 68:

-66.

EI-harzmi MA, Warsy AS. Evaluation of

serum cholesterol and triglyceride level in 1-

- year -old Saudi children. J Trop Paediatr

, 47: 181-185.

Ravi K, Rajasekaran S, Subramanian S. Antihyperlipidemia effect of Eugenia jambolana

seed kernel on streptozotocin-induced

diabetes in rats. Food Chem Toxicol 2005,

: 1433-1439.

Paolisso G, Nenquin M, Schmeer F, Mathot

H, Meissner HP, Henquin JC. Sparteine

increases insulin release by decreasing the K

permeability of the beta-cell membrane.

Biochem Pharmacol 1985, 34: 2355–2361.

Gulfraz M, Mehmmod S, Ahmed A, Fatima

N, Praveen Z, Williamson EM. Comparison

of the antidiabetic activity of Berberis lyceum

root extract and berberine in alloxan-induced

diabetic rats. Phytother Res 2008, 22: 1208-

Mukherjee M, Mukherjee S, Shaw AK,

Ganguly SN. Mukonicine, a carbazole

alkaloid from leaves of Murraya koenigii.

Phytochem 1983, 22: 2328-2329.

Bhattacharya L, Roy SK, Chakraborty DP.

Structure of the carbazole alkaloid

isomurrayazoline from Murraya koenigii.

Phytochem 1982, 21: 2432-2433.

Fiebig M, Pezzuto JM, Soejarto DD,

Kinghorn AD. Koenoline, a further cytotoxic

carbazole alkaloid from Murraya koenigii.

Phytochem 1985, 12: 3041-3043.

Reisch J, Goj O, Wickramasinghe A, Bandara

Herath HMT, Henkel G. Carbazole alkaloids

from seed of Murraya koenigii. Phytochem

, 31: 2877-2879.

Roy S, Chakraborthy DP. Mahanimbine from

Murraya Koenigii Spreng. Phytochem 1974,

: 2893.

Rahman MM, Gray AI. A benzoisofuranone

derivative and carbazole alkaloids from

Murraya koenigii and their antimicrobial

activity. Phytomedicine 2005, 66: 1061-1606.

Adebajo AC, Ayoola, OF, Iwalewa EO,

Akindahunsi AA, Omisore NOA, Adewunmi

CO. Anti-trichomonal, biochemical and

toxicological activities of methanolic extract

and some carbazole alkaloids isolated from

leaves of Murraya koenigii growing in

Nigeria. Phytomedicine 2006, 13: 246-254.

Ito C, Itoigawa M, Nakao K, Murata T,

Tsuboi M, Kaneda N. Induction of apoptosis

by carbazole alkaloids isolated from Murraya

koenigii. Phytomedicine 2006, 13: 359-365.

Arulselvan P, Subramanian SP. Beneficial

effects of Murraya koenigii leaves on

antioxidant defense system and ultra

structural changes of pancreatic beta cell in

experimental diabetes in rats. Chem Biol

Interact 2007, 165: 155-164.

Yadav S, Vats V, Dhunnoo Y, Grover JK.

Hypoglycemic and antihyperglycemic activity

of Murraya koenigii leaves in diabetic rats. J

Ethnopharmacol 2002, 82: 111-116.

Mandal S, Nayak A, Kar M, Banerjee SK,

Das A, Upadhyay SN, Singh RK, Banerji A,

Banerji J. Antidiarrhoeal activity of carbazole

alkaloids from Murraya koenigii Spreng

(Rutaceae) seeds. Fitoterapia 2009 (In press).

David SH, Bell MB. Type 2 diabetes

mellitus: What is the optimal treatment

regimen? Am J Med 2004, 116: 23-29.

McCue P, Vattem D, Shetty K. Inhibitory

effect of clonal oregano extracts against

porcine pancreatic amylase in vitro. Asia Pac

J Clin Nutr 2004, 13: 401-408.

Downloads

Published

09-02-2022

How to Cite

1.
B. Dineshkumar, Analava Mitra, Manjunatha Mahadevappa. Antidiabetic and hypolipidemic effects of mahanimbine (carbazole alkaloid) from murraya koenigii (rutaceae) leaves. ijp [Internet]. 2022 Feb. 9 [cited 2024 Jun. 16];2(1):22-30. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/12

Issue

Section

Original Research Articles