Antibacterial activity of methanolic and acetone extract of some medicinal plants used in indian folklore

Authors

  • J. P. Patel Department of Biology, V.P. and R.P.T.P. Science College, Vallabh Vidyanagar-388120, Gujarat, India.
  • Bharat Gami Department of Biology, V.P. and R.P.T.P. Science College, Vallabh Vidyanagar-388120, Gujarat, India.
  • Kanti Patel Department of Biology, V.P. and R.P.T.P. Science College, Vallabh Vidyanagar-388120, Gujarat, India.
  • Raksha Solanki Department of Biology, V.P. and R.P.T.P. Science College, Vallabh Vidyanagar-388120, Gujarat, India.

Keywords:

Antibacterial activity, Medicinal Plants, infectious diseases

Abstract

Antibacterial study of methanolic and acetone extract in crude and treated (with 50 % lead acetate) form of medicinal plants Alstonia scholaris Linn. R.Br. (Stem bark, Apocynaceae), Achyranthus aspera Linn. (Whole plant, acantheceae), Moringa oleifera Lam. (Leaves, Morinaceae), Tinospora cordifolia (Stem, Menispermaceae), and Enicostema hyssopifolium (Willd) (Stem, Gentianaceae). Extractive values in methanol were found higher then the extractive value in acetone, for all plants. All extract of plants were tried at 40-mg/ml concentrations against eight strains of bacteria, by agarwell-difusion test. Acetone extract was found more active as compared to methanol extract. Phytochemical investigation revealed crude and treated extracts of all plants were containing more or less same type of chemical constituents (except protein and carbohydrate). Selected eight strains of bacteria were study for antibiotic susceptibility against standard antibiotics like Ampicillin (10µg), Tetracycline (25µg), Gentamicin (30µg), Co-Trimoxazole (25µg), Amikacin (10µg), by Octadisc. Treated extract of M. oleifera and A. scholaris were count as to new source of antimicrobial agent for the infectious diseases (Typhoid).

References

Sadqui M, Fushman D and Munoz V.

Atom–by–atom analysis of global downhill

protein folding. Nature. 2006;442:317–321.

Olalde Rangel JA. The systemic theory of

living systems and relevance to CAM. Part

I: the theory. Evid Based Complement

Alternat Med. 2005;2:13–18.

Cragg GM, Boyd MR, Khanna R, Kneller

R, Mays TD, Mazan KD, Newman DJ and

Sausville EA. International collaboration in

drug discovery and development: the NCI

experience. Pure Appl Chem.

;71:1619-1633.

Olila D, Olwa-Odyek and Opuda-Asibo J.

Antibacterial and antifungal activities of

extracts of Zanthoxylum chalybeum and

Warburgia ugandensis, Ugandan medicinal

plants, African Health Sciences.

;1(2):66-72.

Fauci A. New and reemerging diseases: The

importance of biomedical research.

Emerging Infectious Diseases.

(www.cdc.gov/ncidod/EID/vol4no3/fauci).

;4:3.

Robert A and Meunier B. Is Alkylation the

Main Mechanism of Action of the

Antimalarial Drug Artemisinin. Chem. Soc.

Rev. 1998;27:273-279.

Setti EL, Micetich RG. New Trends in

Antimicrobial Development. Current

Medicinal Chemistry. 1998;5:101-113.

WHO Geneva, Quality Control Method for

Medicinal Plant Materials, New Delhi.

;51:30.

Harbone JB. Phytochemical Methods – A

Guide to Modern Techniques of plant

analysis 2nd edition. (Chapman and Hall

London. New York) 1984.

Perez C, Paulin Mand and Bazerque P. An

antibiotic assay by the well agar method.

Acta. Biological Medicine Experitimentalis.

;15:113-115.

Ibrahim MB, Owonubi MO and Onaolapo

JA. Antimicrobial effectsof extracts of leaf,

stem, and root-bark of Anogiessus leicarpus

onStaphylococcus aureus NCTC 8190,

Escherichia coli NCTC 10418and Proteus

vulgaris NCTC 4636. J. Pharmaceutical

Res. Dev. 1997;2:20-26.

Okeke MI, Iroegbu CU, Eze EN, Okoli AS

and Esimone CO. Evaluation of extracts of

the root of Landolphia owerrience for

antibacterial activity. J. Ethnopharmacol.

;78:119-127.

Okoli AS, Okeke MI, Iroegbu CU and Ebo

PU. Antibacterial activityof Harungana

madagascariensis leaf extracts. Phytother.

Res. 2002;16:174-179.

Scalbert A. Antimicrobial properties of

tannins. Phytochemistry. 1991;30:3875-

Aboaba OO, Smith SI and Olude FO.

Antibacterial Effect of Edible Plant Extract

on Escherichia coli 0157:H7, Pakistan

Journal of Nutrition. 2006;5(4):325-327.

Aboaba OO and Efuwape BM.

Antibacterial properties of some Nigerian

spices. Bio. Res. Comm. 2001;13:183-188.

Hostettman K and Nakanishi K. Moronic

acid, a simple triterpenoid keto acid with

antimicrobial activity isolated from Ozoroa

Mucroanta. J. Med. Plant Res.

;31:358-366.

Leven M, VandenBerghe DA, Mertens F,

Vlictinck A and Lammens E. Screening of

higher plants for biological activities/-

antimicrobial activity. Plant. Med.

;36:311-321.

Damintoti Karou1, Aly Savadogo,

Antonella Canini, Saydou Yameogo, Carla

Montesano, Jacques Simpore, Vittorio

Colizzi and Alfred S. Traore. Antibacterial

activity of alkaloids from Sida acuta.

African Journal of Biotechnology.

;4(12):1452-1457.

Eloff JN. Which extractan should be used

for screening and isolation of antimicrobial

components from plants. J.

Ethanopharmacol. 1998;60:1-8.

Cowan MM. Plant product as antimicrobial

agents, Clin.Microbiol.Rev. 1999;12:564-

Nikaido H and Vaara M. “Molecular Basis

of Bacterial Outer Membrane

Permeability”, Microbiological Reviews.

;1:1-32.

Jigna P, Rathish N and Sumita C.

Priliminary screening of some folklore

medicinal plants from western India for

potential antimicrobial activity, Inian J.

Pharmacol. 2005;37(6):408-409.

Khan MR, Omoloso AD and Kihara M.

Antibacterial activity of Alstonia scholaris

and Leea tetramera, Fitoterpia. 2003;74(7-

:736-40.

Goyal MM and Varshney A. Effects of

natural products isolated from three species

of Alstonia on some gram-positive and

gram-negative bacteria. Indian Drugs

;32(2):69-72.

Clark WS. Antimicrobial activities of

phenolic constituents of Magnolia

grandiflora L J. Pharm. Sci. 1981;70:951-

Doughari JH, Pukuma MS and De N.

Antibacterial effects of Balanites aegyptiaca

L. Drel. and Moringa oleifera Lam. on

Salmonella typhi, African Journal of

Biotechnology. 2007;6(19):2212-2215.

Chin NT, Perry CM, Ly NT, Ha HD, Thong

M and Diep TS. Randomized controlled

comparison of azithromycin and ofloxacin

for treatment of multidrug resistant or

nalidixic acid resistant enteric fever.

Antimicrob. Agents Chemother.

;44:1855-1859.

Benoit D, Renand L, Daniele M, Ame B,

David B, Michael RM, Elisabeth C and

Anel C. Variant Salmonella genomic island

antibitotic gene resistance cluster in

Salmonella enterica Albany. Emerg. Infect.

Dis. 2003;9(5):585-591.

Abdullah WB, Anowa H, Doli G, Amina

TS, Kamrun N, Korshed A, Neor A, Aliya

N, Balakrish N, Stephen L and Robert B.

Bacteremic typhoid fever in children in an

urban slum, Bangladesh. Emerg. Infect. Dis.

;11(2):326-329.

Ghosal S and Jaiswal DK. Chemical

constituents of gentianaceae

XXVIII:flavonoids of Enicostema

hyssopifolium (Willd.) Verd. J.Pharm Sci.

;69(1):53-56.

Singh SS, Pandey SC, Srivastava S, Gupta

VS, Patro B and Ghosh AC. Chemistry and

Medicinal Properties of Tinospora

cordifolia. Indian Journal of Pharmacology.

;35:83-91.

Rege NN, Nazareth HM, Bapat RD and

Dahanukar SA. Modulation of

immunosuppression in obstructive jaundice

by Tinospora cordifolia. Indian J Med Res.

;90:478-83.

Franklin TJ, Snow GA, Barrett-Bee KJ and

Nolan RD Biochemistry of antimicrobial

action. Fourth edition. Chapman and Hall

London New York. 1987;112:pp. 71-73.

Cohen ML. Epidemiology of drug

resistance: implications for a

postantimicrobial era. Science.

;257:1050-1055.

Anesini E and Perez C. Screening of plants

used in Argentine folk medicine for

antimicrobial activity. J. Ethnopharmacol.

;39:119-128.

TLG Monte FJQ, Matos FJA, Alencar JW,

Craveiro AA, Barbosa RCSB and Lima ED.

Chemical composition and antimicrobial

activity of essencial oils from Brazilian

plants. Fitoterapia. 1992;63:266-268.

Martinez MJ, Betancourt J, AlonsoGonzalez N and Jauregui A. Screening of

some Cuban medicinal plants for

antimicrobial activity. J. Ethnopharmacol.

;52:171-174.

Martinez MJ, Vasquez SM, Espinosa-Perez

C, Dias M and Herrera- Sanchez M.

Antimicrobial properties of argentatine A

isolated from Parthenium argentatum.

Fitoterapia. 1994;65:371-372.

Downloads

Published

30-06-2011

How to Cite

1.
J. P. Patel, Bharat Gami, Kanti Patel, Raksha Solanki. Antibacterial activity of methanolic and acetone extract of some medicinal plants used in indian folklore. ijp [Internet]. 2011 Jun. 30 [cited 2024 Feb. 22];3(2):261-9. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/103

Issue

Section

Original Research Articles